6ifa: Difference between revisions
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==Structure of beta-trefoil lectin from Entamoeba histolytica== | |||
<StructureSection load='6ifa' size='340' side='right'caption='[[6ifa]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6ifa]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica_HM-1:IMSS Entamoeba histolytica HM-1:IMSS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IFA FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ifa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ifa OCA], [https://pdbe.org/6ifa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ifa RCSB], [https://www.ebi.ac.uk/pdbsum/6ifa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ifa ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/N9TFI9_ENTH1 N9TFI9_ENTH1] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
beta-trefoil lectins are galactose/N-acetyl galactosamine specific lectins, which are widely distributed across all kingdoms of life and are known to perform several important functions. However, there is no report available on the characterization of these lectins from protozoans. We have performed structural and biophysical studies on a beta-trefoil lectin from Entamoeba histolytica (EntTref) which exists as a mixture of monomers and dimers in solution. Further, we have determined the affinities of EntTref for rhamnose, galactose and different galactose-linked sugars. We obtained the crystal structure of EntTref in a sugar-free form (EntTref_apo) and a rhamnose-bound form (EntTref_rham). A novel Cys residue-mediated dimerization was revealed in the crystal structure of EntTref_apo while the structure of EntTref_rham provided the structural basis for the recognition of rhamnose by a beta-trefoil lectin for the first time. To the best of our knowledge, this is the only report of the structural, functional and biophysical characterization of a beta-trefoil lectin from a protozoan source and the first report of Cys-mediated dimerization in this class of lectins. | |||
Crystal structures of a beta-trefoil lectin from Entamoeba histolytica in monomeric and a novel disulphide bond-mediated dimeric forms.,Khan F, Kurre D, Suguna K Glycobiology. 2020 Jan 21. pii: 5710858. doi: 10.1093/glycob/cwaa001. PMID:31967310<ref>PMID:31967310</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6ifa" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Entamoeba histolytica HM-1:IMSS]] | |||
[[Category: Large Structures]] | |||
[[Category: Khan F]] | |||
[[Category: Suguna K]] | |||
Latest revision as of 13:03, 23 October 2024
Structure of beta-trefoil lectin from Entamoeba histolyticaStructure of beta-trefoil lectin from Entamoeba histolytica
Structural highlights
FunctionPublication Abstract from PubMedbeta-trefoil lectins are galactose/N-acetyl galactosamine specific lectins, which are widely distributed across all kingdoms of life and are known to perform several important functions. However, there is no report available on the characterization of these lectins from protozoans. We have performed structural and biophysical studies on a beta-trefoil lectin from Entamoeba histolytica (EntTref) which exists as a mixture of monomers and dimers in solution. Further, we have determined the affinities of EntTref for rhamnose, galactose and different galactose-linked sugars. We obtained the crystal structure of EntTref in a sugar-free form (EntTref_apo) and a rhamnose-bound form (EntTref_rham). A novel Cys residue-mediated dimerization was revealed in the crystal structure of EntTref_apo while the structure of EntTref_rham provided the structural basis for the recognition of rhamnose by a beta-trefoil lectin for the first time. To the best of our knowledge, this is the only report of the structural, functional and biophysical characterization of a beta-trefoil lectin from a protozoan source and the first report of Cys-mediated dimerization in this class of lectins. Crystal structures of a beta-trefoil lectin from Entamoeba histolytica in monomeric and a novel disulphide bond-mediated dimeric forms.,Khan F, Kurre D, Suguna K Glycobiology. 2020 Jan 21. pii: 5710858. doi: 10.1093/glycob/cwaa001. PMID:31967310[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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