5fxu: Difference between revisions
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The | ==Crystal Structure of Puumala virus Gn glycoprotein ectodomain== | ||
<StructureSection load='5fxu' size='340' side='right'caption='[[5fxu]], [[Resolution|resolution]] 2.28Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5fxu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Orthohantavirus_puumalaense Orthohantavirus puumalaense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FXU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.28Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fxu OCA], [https://pdbe.org/5fxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fxu RCSB], [https://www.ebi.ac.uk/pdbsum/5fxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fxu ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9WJ31_9VIRU Q9WJ31_9VIRU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative agent of hemorrhagic fever with renal syndrome. Fitting of PUUV Gn into an electron cryomicroscopy reconstruction of intact Gn-Gc spike complexes from the closely related but non-pathogenic Tula hantavirus localized Gn tetramers to the membrane-distal surface of the virion. The accuracy of the fitting was corroborated by epitope mapping and genetic analysis of available PUUV sequences. Interestingly, Gn exhibits greater non-synonymous sequence diversity than the less accessible Gc, supporting a role of the host humoral immune response in exerting selective pressure on the virus surface. The fold of PUUV Gn is likely to be widely conserved across hantaviruses. | |||
A Molecular-Level Account of the Antigenic Hantaviral Surface.,Li S, Rissanen I, Zeltina A, Hepojoki J, Raghwani J, Harlos K, Pybus OG, Huiskonen JT, Bowden TA Cell Rep. 2016 May 3;15(5):959-67. doi: 10.1016/j.celrep.2016.03.082. Epub 2016, Apr 21. PMID:27117403<ref>PMID:27117403</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5fxu" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Orthohantavirus puumalaense]] | ||
[[Category: | [[Category: Bowden TA]] | ||
[[Category: | [[Category: Harlos K]] | ||
[[Category: Hepojoki J]] | |||
[[Category: Huiskonen JT]] | |||
[[Category: Li S]] | |||
[[Category: Pybus OG]] | |||
[[Category: Raghwani J]] | |||
[[Category: Rissanen I]] | |||
[[Category: Zeltina A]] | |||