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| | <StructureSection load='3kz1' size='340' side='right' caption='Human Rho guanine nucleotide exchange factor 11 DH/PH domain (grey) complex with transforming protein RhoA (cyan), GSP and Mg+2 ion (green) (PDB code [[3kz1]])' scene=''> |
| | __TOC__ |
| | == Function == |
| | '''Rho guanine nucleotide exchange factor''' (GEF) stimulate the exchange of the inactive GDP-bound GTPase to its active GTP-bound form. Binding of GEF to GDP-GTPase catalyzes the dissociation of GDP from the protein<ref>PMID:23303910</ref>. GEF domains include the catalytic DH/PH (Dbl Homology/Pleckstrin Homology) domain; the CH (Calponin Homology) domain is believed to be involved in calcium regulation; the SH3 domain interacts with proline-rich sequences. |
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| <StructureSection load='3kz1' size='340' side='right' caption='Human Rho guanine nucleotide exchange factor 11 DH/PH domain complex with transforming protein RhoA (PDB code [[3kz1]])' scene=''> | | *'''Rho guanine nucleotide exchange factor 1''' binds to G proteins and stimulate Rho-dependent signals.<br /> |
| '''Rho guanine nucleotide exchange factor''' (Rho) stimulate the exchange of the inactive GDP-bound GTPase to its active GTP-bound form. Binding of Rho to GDP-GTPase catalyzes the dissociation of GDP from the protein. Rho domains include the catalytic DH/PH (Dbl Homology/Pleckstrin Homology) domain; the CH (Calponin Homology) domain is believed to be involved in calcium regulation; the SH3 domain interacts with proline-rich sequences. | | *'''Rho guanine nucleotide exchange factor 3''' acts on RHOA and RHOB involved in bone biology.<br /> |
| | | *'''Rho guanine nucleotide exchange factor 6''' and '''Rho guanine nucleotide exchange factor 7''' act on Rac1.<br /> |
| == Function ==
| | *'''Rho guanine nucleotide exchange factor 9''' mutations involved in developmental and epileptic encephalopathy<ref>PMID:35638461</ref>. |
| | *'''Rho guanine nucleotide exchange factor 11''' mediates Rho-myosin light chain signaling pathways at cell-cell junctions<ref>PMID:22665792</ref>. |
| | *'''Rho guanine nucleotide exchange factor 18''' regulates tissue architecture and function via RhoA activation<ref>PMID:23698346</ref>. |
| | *'''Rho guanine nucleotide exchange factor 25''' involved in contraction of vascular smooth muscle. |
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| == Disease == | | == Disease == |
| | | Increased GEF activity is involved in cancer cell proliferation by affecting Rho GTPases <ref>PMID:24037532</ref>. |
| == Relevance == | | == 3D Structures of Rho guanine nucleotide exchange factor == |
| | | [[Rho guanine nucleotide exchange factor 3D structures]] |
| == Structural highlights ==
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| </StructureSection> | | </StructureSection> |
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| == 3D Structures of Rho guanine nucleotide exchange factor ==
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *Rho guanine nucleotide exchange factor 1 (P115-RhoGEF)
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| **[[1iap]] – hRho N terminal - human<br />
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| **[[3odo]], [[3odw]], [[3odx]] – hRho DH/PH domain <br />
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| **[[3p6a]] – hRho DH/PH domain (mutant)<br />
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| **[[1shz]], [[3ab3]] – hRho N terminal + guanine nucleotide-binding protein<br />
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| *Rho guanine nucleotide exchange factor 4 (Asef)
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| **[[2dx1]], [[2pz1]] – hRho <br />
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| **[[3nmx]], [[3nmz]] – hRho + APC variant protein<br />
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| *Rho guanine nucleotide exchange factor 6
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| **[[1ujy]] – hRho SH3 domain - NMR<br />
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| **[[1wyr]] – hRho CH domain - NMR<br />
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| *Rho guanine nucleotide exchange factor 7 (β-PIX)
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| **[[1by1]] – hRho DH domain - NMR<br />
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| **[[2l3g]] – hRho CH domain - NMR<br />
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| **[[2esw]] – Rho SH3 domain – mouse<br />
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| **[[2g6f]] – rRho SH3 domain – rat<br />
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| **[[2w6b]] – rRho coiled-coil domain <br />
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| *Rho guanine nucleotide exchange factor 7 complex with peptide
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| **[[2ak5]] – hRho SH3 domain + CBL-b peptide <br />
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| **[[2df6]] – hRho SH3 domain + PAK-1 peptide <br />
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| **[[1zsg]] – hRho residues 179-243 + PAK-1 peptide – NMR<br />
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| **[[2p4r]] – rRho SH3 domain + E3 ubiquitin protein ligase peptide <br />
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| **[[3l4f]] – rRho coiled-coil domain + SHANK1 PDZ domain <br />
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| *Rho guanine nucleotide exchange factor 8 (PRONE8)
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| **[[2ntx]] – AtRho PRONE domain – ''Arabidopasis thaliana''<br />
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| **[[2wbl]] – AtRho PRONE domain + GTPase ROP7 <br />
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| **[[2nty]] – AtRho PRONE domain + GTPase ROP4 + GDP <br />
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| **[[3eo2]] – hRho residues 161-373 <br />
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| **[[4xh9]] – hRho DH/PH domain + transforming protein RhoA <br />
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| *Rho guanine nucleotide exchange factor 9 (collybistin)
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| **[[2ysq]] – hRho SH3 domain - NMR<br />
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| **[[4mt6]], [[4mt7]] – rRho (mutant)<br />
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| **[[2dfk]] – rRho + CDC42 <br />
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| *Rho guanine nucleotide exchange factor 11
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| **[[2dls]] – hRho PDZ domain - NMR<br />
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| **[[3kz1]], [[3t06]] – hRho DH/PH domain + transforming protein RhoA <br />
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| **[[1xcg]] – hRho DH/PH domain + transforming protein RhoA - NMR<br />
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| **[[3cx6]] – rRho RGS domain + guanine nucleotide-binding protein <br />
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| *Rho guanine nucleotide exchange factor 12 (Leukemia-associated RhoGEF)
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| **[[1txd]] – hRho DH/PH domain <br />
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| **[[2omj]] – hRho PDZ domain <br />
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| **[[1x86]] – hRho DH/PH domain (mutant) + transforming protein RhoA <br />
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| **[[2os6]] – hRho PDZ domain + plexin peptide – NMR<br />
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| *Rho guanine nucleotide exchange factor 16
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| **[[1x6b]] – hRho SH3 domain - NMR<br />
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| | [[Category:Topic Page]] |