5c6j: Difference between revisions

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-'''Unreleased structure'''
-The entry 5c6j is ON HOLD
+==Crystal Structure of Gadolinium derivative of HEWL solved using Free-Electron Laser radiation==
+<StructureSection load='5c6j' size='340' side='right'caption='[[5c6j]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5c6j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C6J FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DO3:10-((2R)-2-HYDROXYPROPYL)-1,4,7,10-TETRAAZACYCLODODECANE+1,4,7-TRIACETIC+ACID'>DO3</scene>, <scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6j OCA], [https://pdbe.org/5c6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c6j RCSB], [https://www.ebi.ac.uk/pdbsum/5c6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6j ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential 'bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.
-Authors: Galli, L., Barends, T.R.M., Son, S.-K., White, T.A., Barty, A., Botha, S., Boutet, S., Caleman, C., Doak, R.B., Nanao, M.H., Nass, K., Shoeman, R.L., Timneanu, N., Santra, R., Schlichting, I., Chapman, H.N.
+Towards phasing using high X-ray intensity.,Galli L, Son SK, Barends TR, White TA, Barty A, Botha S, Boutet S, Caleman C, Doak RB, Nanao MH, Nass K, Shoeman RL, Timneanu N, Santra R, Schlichting I, Chapman HN IUCrJ. 2015 Sep 30;2(Pt 6):627-634. eCollection 2015 Nov 1. PMID:26594370<ref>PMID:26594370</ref>
-Description: Crystal Structure of Gadolinium derivative of HEWL solved using Free-Electron Laser radiation
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Santra, R]]
+<div class="pdbe-citations 5c6j" style="background-color:#fffaf0;"></div>
-[[Category: Caleman, C]]
-[[Category: Barty, A]]
+==See Also==
-[[Category: Galli, L]]
+*[[Lysozyme 3D structures|Lysozyme 3D structures]]
-[[Category: Shoeman, R.L]]
+== References ==
-[[Category: Nanao, M.H]]
+<references/>
-[[Category: Timneanu, N]]
+__TOC__
-[[Category: Botha, S]]
+</StructureSection>
-[[Category: Nass, K]]
+[[Category: Gallus gallus]]
-[[Category: Son, S.-K]]
+[[Category: Large Structures]]
-[[Category: White, T.A]]
+[[Category: Barends TRM]]
-[[Category: Barends, T.R.M]]
+[[Category: Barty A]]
-[[Category: Boutet, S]]
+[[Category: Botha S]]
-[[Category: Chapman, H.N]]
+[[Category: Boutet S]]
-[[Category: Doak, R.B]]
+[[Category: Caleman C]]
-[[Category: Schlichting, I]]
+[[Category: Chapman HN]]
+[[Category: Doak RB]]
+[[Category: Galli L]]
+[[Category: Nanao MH]]
+[[Category: Nass K]]
+[[Category: Santra R]]
+[[Category: Schlichting I]]
+[[Category: Shoeman RL]]
+[[Category: Son S-K]]
+[[Category: Timneanu N]]
+[[Category: White TA]]