4z7u: Difference between revisions

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-'''Unreleased structure'''
-The entry 4z7u is ON HOLD  until Paper Publication
+==S13 complex==
+<StructureSection load='4z7u' size='340' side='right'caption='[[4z7u]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4z7u]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z7U FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7u OCA], [https://pdbe.org/4z7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z7u RCSB], [https://www.ebi.ac.uk/pdbsum/4z7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7u ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q30069_HUMAN Q30069_HUMAN]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In HLA-DQ8-associated celiac disease (CD), the pathogenic T cell response is directed toward an immunodominant alpha-gliadin-derived peptide (DQ8-glia-alpha1). However, our knowledge of TCR gene usage within the primary intestinal tissue of HLA-DQ8+ CD patients is limited. We identified two populations of HLA-DQ8-glia-alpha1 tetramer+ CD4+ T cells that were essentially undetectable in biopsy samples from patients on a gluten-free diet but expanded rapidly and specifically after antigenic stimulation. Distinguished by expression of TRBV9, both T cell populations displayed biased clonotypic repertoires and reacted similarly against HLA-DQ8-glia-alpha1. In particular, TRBV9 paired most often with TRAV26-2, whereas the majority of TRBV9- TCRs used TRBV6-1 with no clear TRAV gene preference. Strikingly, both tetramer+/TRBV9+ and tetramer+/TRBV9- T cells possessed a non-germline-encoded arginine residue in their CDR3alpha and CDR3beta loops, respectively. Comparison of the crystal structures of three TRBV9+ TCRs and a TRBV9- TCR revealed that, as a result of distinct TCR docking modes, the HLA-DQ8-glia-alpha1 contacts mediated by the CDR3-encoded arginine were almost identical between TRBV9+ and TRBV9- TCRs. In all cases, this interaction centered on two hydrogen bonds with a specific serine residue in the bound peptide. Replacement of serine with alanine at this position abrogated TRBV9+ and TRBV9- clonal T cell proliferation in response to HLA-DQ8-glia-alpha1. Gluten-specific memory CD4+ T cells with structurally and functionally conserved TCRs therefore predominate in the disease-affected tissue of patients with HLA-DQ8-mediated CD.
-Authors: Petersen, J., Rossjohn, J., Reid, H.H., Koning, F.
+Determinants of Gliadin-Specific T Cell Selection in Celiac Disease.,Petersen J, van Bergen J, Loh KL, Kooy-Winkelaar Y, Beringer DX, Thompson A, Bakker SF, Mulder CJ, Ladell K, McLaren JE, Price DA, Rossjohn J, Reid HH, Koning F J Immunol. 2015 May 6. pii: 1500161. PMID:25948817<ref>PMID:25948817</ref>
-Description: S13 complex
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Rossjohn, J]]
+<div class="pdbe-citations 4z7u" style="background-color:#fffaf0;"></div>
-[[Category: Reid, H.H]]
-[[Category: Petersen, J]]
+==See Also==
-[[Category: Koning, F]]
+*[[Gluten|Gluten]]
+*[[MHC 3D structures|MHC 3D structures]]
+*[[MHC II 3D structures|MHC II 3D structures]]
+*[[T-cell receptor 3D structures|T-cell receptor 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Triticum aestivum]]
+[[Category: Koning F]]
+[[Category: Petersen J]]
+[[Category: Reid HH]]
+[[Category: Rossjohn J]]