4z39: Difference between revisions
New page: '''Unreleased structure''' The entry 4z39 is ON HOLD Authors: Northey, T., Venthur, H., De Biasio, F., Chauviac, F.-X., Cole, A.R., Field, L.M., Zhou, J.-J., Keep, N.H. Description: St... |
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The | ==Structure of OBP3 from the vetch aphid Megoura viciae== | ||
<StructureSection load='4z39' size='340' side='right'caption='[[4z39]], [[Resolution|resolution]] 1.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4z39]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Megoura_viciae Megoura viciae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z39 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z39 OCA], [https://pdbe.org/4z39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z39 RCSB], [https://www.ebi.ac.uk/pdbsum/4z39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z39 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0S2E5N6_MEGVI A0A0S2E5N6_MEGVI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Aphids use chemical cues to locate hosts and find mates. The vetch aphid Megoura viciae feeds exclusively on the Fabaceae, whereas the currant-lettuce aphid Nasonovia ribisnigri alternates hosts between the Grossulariaceae and Asteraceae. Both species use alarm pheromones to warn of dangers. For N. ribisnigri this pheromone is a single component (E)-beta-farnesene but M. viciae uses a mixture of (E)-beta-farnesene, (-)-alpha-pinene, beta-pinene, and limonene. Odorant-binding proteins (OBP) are believed to capture and transport such semiochemicals to their receptors. Here, we report the first aphid OBP crystal structures and examine their molecular interactions with the alarm pheromone components. Our study reveals some unique structural features: 1) the lack of an internal ligand binding site; 2) a striking groove in the surface of the proteins as a putative binding site; 3) the N-terminus rather than the C-terminus occupies the site closing off the conventional OBP pocket. The results from fluorescent binding assays, molecular docking and dynamics demonstrate that OBP3 from M. viciae can bind to all four alarm pheromone components and the differential ligand binding between these very similar OBP3s from the two aphid species is determined mainly by the direct pi-pi interactions between ligands and the aromatic residues of OBP3s in the binding pocket. | |||
Crystal Structures and Binding Dynamics of Odorant-Binding Protein 3 from two aphid species Megoura viciae and Nasonovia ribisnigri.,Northey T, Venthur H, De Biasio F, Chauviac FX, Cole A, Ribeiro KA Junior, Grossi G, Falabella P, Field LM, Keep NH, Zhou JJ Sci Rep. 2016 Apr 22;6:24739. doi: 10.1038/srep24739. PMID:27102935<ref>PMID:27102935</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 4z39" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: De Biasio | ==See Also== | ||
[[Category: Field | *[[Odorant binding protein 3D structures|Odorant binding protein 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Venthur | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Megoura viciae]] | |||
[[Category: Chauviac F-X]] | |||
[[Category: Cole AR]] | |||
[[Category: De Biasio F]] | |||
[[Category: Field LM]] | |||
[[Category: Keep NH]] | |||
[[Category: Northey T]] | |||
[[Category: Venthur H]] | |||
[[Category: Zhou J-J]] | |||
Latest revision as of 11:39, 23 October 2024
Structure of OBP3 from the vetch aphid Megoura viciaeStructure of OBP3 from the vetch aphid Megoura viciae
Structural highlights
FunctionPublication Abstract from PubMedAphids use chemical cues to locate hosts and find mates. The vetch aphid Megoura viciae feeds exclusively on the Fabaceae, whereas the currant-lettuce aphid Nasonovia ribisnigri alternates hosts between the Grossulariaceae and Asteraceae. Both species use alarm pheromones to warn of dangers. For N. ribisnigri this pheromone is a single component (E)-beta-farnesene but M. viciae uses a mixture of (E)-beta-farnesene, (-)-alpha-pinene, beta-pinene, and limonene. Odorant-binding proteins (OBP) are believed to capture and transport such semiochemicals to their receptors. Here, we report the first aphid OBP crystal structures and examine their molecular interactions with the alarm pheromone components. Our study reveals some unique structural features: 1) the lack of an internal ligand binding site; 2) a striking groove in the surface of the proteins as a putative binding site; 3) the N-terminus rather than the C-terminus occupies the site closing off the conventional OBP pocket. The results from fluorescent binding assays, molecular docking and dynamics demonstrate that OBP3 from M. viciae can bind to all four alarm pheromone components and the differential ligand binding between these very similar OBP3s from the two aphid species is determined mainly by the direct pi-pi interactions between ligands and the aromatic residues of OBP3s in the binding pocket. Crystal Structures and Binding Dynamics of Odorant-Binding Protein 3 from two aphid species Megoura viciae and Nasonovia ribisnigri.,Northey T, Venthur H, De Biasio F, Chauviac FX, Cole A, Ribeiro KA Junior, Grossi G, Falabella P, Field LM, Keep NH, Zhou JJ Sci Rep. 2016 Apr 22;6:24739. doi: 10.1038/srep24739. PMID:27102935[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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