4ylo: Difference between revisions

<StructureSection load='4ylo' size='340' side='right'caption='[[4ylo]], [[Resolution|resolution]] 6.00&Aring;' scene=''>

<table><tr><td colspan='2'>[[4ylo]] is a 27 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YLO FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 6&#8491;</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ylo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ylo OCA], [https://pdbe.org/4ylo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ylo RCSB], [https://www.ebi.ac.uk/pdbsum/4ylo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ylo ProSAT]</span></td></tr>

[https://www.uniprot.org/uniprot/RPOA_ECO24 RPOA_ECO24] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

<div style="background-color:#fffaf0;">

During transcription initiation, RNA polymerase binds to promoter DNA to form an initiation complex containing a DNA bubble and enters into abortive cycles of RNA synthesis before escaping the promoter to transit into the elongation phase for processive RNA synthesis. Here we present the crystal structures of E. coli transcription initiation complexes containing a complete transcription bubble and de novo synthesized RNA oligonucleotides at about 6-A resolution. The structures show how RNA polymerase recognizes DNA promoters that contain spacers of different lengths and reveal a bridging interaction between the 5'-triphosphate of the nascent RNA and the sigma factor that may function to stabilize the short RNA-DNA hybrids during the early stage of transcription initiation. The conformation of the RNA oligonucleotides and the paths of the DNA strands in the complete initiation complexes provide insights into the mechanism that controls both the abortive and productive RNA synthesis.

 

Crystal Structures of the E. coli Transcription Initiation Complexes with a Complete Bubble.,Zuo Y, Steitz TA Mol Cell. 2015 Apr 8. pii: S1097-2765(15)00175-6. doi:, 10.1016/j.molcel.2015.03.010. PMID:25866247<ref>PMID:25866247</ref>

 

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<div class="pdbe-citations 4ylo" style="background-color:#fffaf0;"></div>

 

*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]

*[[Sigma factor 3D structures|Sigma factor 3D structures]]

[[Category: Escherichia coli]]

[[Category: Large Structures]]

[[Category: Synthetic construct]]

[[Category: Steitz TA]]

[[Category: Zuo Y]]