4qi8: Difference between revisions
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==Lytic polysaccharide monooxygenase 9F from Neurospora crassa, NcLPMO9F== | ==Lytic polysaccharide monooxygenase 9F from Neurospora crassa, NcLPMO9F== | ||
<StructureSection load='4qi8' size='340' side='right' caption='[[4qi8]], [[Resolution|resolution]] 1.10Å' scene=''> | <StructureSection load='4qi8' size='340' side='right'caption='[[4qi8]], [[Resolution|resolution]] 1.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4qi8]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4qi8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QI8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QI8 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | ||
< | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qi8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qi8 OCA], [https://pdbe.org/4qi8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qi8 RCSB], [https://www.ebi.ac.uk/pdbsum/4qi8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qi8 ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/LP9F_NEUCR LP9F_NEUCR] Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 oxidation products (PubMed:23102010, PubMed:31835532, PubMed:35080911). Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate (By similarity).[UniProtKB:Q7SHI8]<ref>PMID:23102010</ref> <ref>PMID:31835532</ref> <ref>PMID:35080911</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A new paradigm for cellulose depolymerization by fungi focuses on an oxidative mechanism involving cellobiose dehydrogenases (CDH) and copper-dependent lytic polysaccharide monooxygenases (LPMO); however, mechanistic studies have been hampered by the lack of structural information regarding CDH. CDH contains a haem-binding cytochrome (CYT) connected via a flexible linker to a flavin-dependent dehydrogenase (DH). Electrons are generated from cellobiose oxidation catalysed by DH and shuttled via CYT to LPMO. Here we present structural analyses that provide a comprehensive picture of CDH conformers, which govern the electron transfer between redox centres. Using structure-based site-directed mutagenesis, rapid kinetics analysis and molecular docking, we demonstrate that flavin-to-haem interdomain electron transfer (IET) is enabled by a haem propionate group and that rapid IET requires a closed CDH state in which the propionate is tightly enfolded by DH. Following haem reduction, CYT reduces LPMO to initiate oxygen activation at the copper centre and subsequent cellulose depolymerization. | |||
Structural basis for cellobiose dehydrogenase action during oxidative cellulose degradation.,Tan TC, Kracher D, Gandini R, Sygmund C, Kittl R, Haltrich D, Hallberg BM, Ludwig R, Divne C Nat Commun. 2015 Jul 7;6:7542. doi: 10.1038/ncomms8542. PMID:26151670<ref>PMID:26151670</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4qi8" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Monooxygenase 3D structures|Monooxygenase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Divne | [[Category: Neurospora crassa]] | ||
[[Category: Gandini | [[Category: Divne C]] | ||
[[Category: Hallberg | [[Category: Gandini R]] | ||
[[Category: Haltrich | [[Category: Hallberg BM]] | ||
[[Category: Kittl | [[Category: Haltrich D]] | ||
[[Category: Ludwig | [[Category: Kittl R]] | ||
[[Category: Sygmund | [[Category: Ludwig R]] | ||
[[Category: Tan | [[Category: Sygmund C]] | ||
[[Category: Tan TC]] | |||