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==High-speed fixed-target serial virus crystallography== | ==High-speed fixed-target serial virus crystallography== | ||
<StructureSection load='5mqw' size='340' side='right' caption='[[5mqw]], [[Resolution|resolution]] 2.40Å' scene=''> | <StructureSection load='5mqw' size='340' side='right'caption='[[5mqw]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5mqw]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5mqw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Operophtera_brumata_cypovirus_18 Operophtera brumata cypovirus 18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MQW FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mqw OCA], [https://pdbe.org/5mqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mqw RCSB], [https://www.ebi.ac.uk/pdbsum/5mqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mqw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q30C70_9REOV Q30C70_9REOV] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 17: | Line 19: | ||
</div> | </div> | ||
<div class="pdbe-citations 5mqw" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5mqw" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Polyhedrin|Polyhedrin]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Operophtera brumata cypovirus 18]] | [[Category: Operophtera brumata cypovirus 18]] | ||
[[Category: Alonso-Mori | [[Category: Alonso-Mori R]] | ||
[[Category: Brewster | [[Category: Brewster AS]] | ||
[[Category: Damiani | [[Category: Damiani DS]] | ||
[[Category: David | [[Category: David C]] | ||
[[Category: Duman | [[Category: Duman R]] | ||
[[Category: Fischer | [[Category: Fischer P]] | ||
[[Category: Fry | [[Category: Fry EE]] | ||
[[Category: Ginn | [[Category: Ginn HM]] | ||
[[Category: Harlos | [[Category: Harlos K]] | ||
[[Category: Meents | [[Category: Meents A]] | ||
[[Category: Meyer | [[Category: Meyer J]] | ||
[[Category: Michels-Clark | [[Category: Michels-Clark T]] | ||
[[Category: Nelson | [[Category: Nelson S]] | ||
[[Category: Pakendorf | [[Category: Pakendorf T]] | ||
[[Category: Reime | [[Category: Reime B]] | ||
[[Category: Ren | [[Category: Ren J]] | ||
[[Category: Roedig | [[Category: Roedig P]] | ||
[[Category: Sauter | [[Category: Sauter NK]] | ||
[[Category: Sikorsky | [[Category: Sikorsky M]] | ||
[[Category: Stuart | [[Category: Stuart DI]] | ||
[[Category: Sutton | [[Category: Sutton G]] | ||
[[Category: Vartiainen | [[Category: Vartiainen I]] | ||
[[Category: Wagner | [[Category: Wagner A]] | ||
[[Category: Walter | [[Category: Walter TS]] | ||
[[Category: Warmer | [[Category: Warmer M]] | ||
[[Category: Young | [[Category: Young ID]] | ||
Latest revision as of 07:25, 21 November 2024
High-speed fixed-target serial virus crystallographyHigh-speed fixed-target serial virus crystallography
Structural highlights
FunctionPublication Abstract from PubMedWe report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our method allows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities. High-speed fixed-target serial virus crystallography.,Roedig P, Ginn HM, Pakendorf T, Sutton G, Harlos K, Walter TS, Meyer J, Fischer P, Duman R, Vartiainen I, Reime B, Warmer M, Brewster AS, Young ID, Michels-Clark T, Sauter NK, Kotecha A, Kelly J, Rowlands DJ, Sikorsky M, Nelson S, Damiani DS, Alonso-Mori R, Ren J, Fry EE, David C, Stuart DI, Wagner A, Meents A Nat Methods. 2017 Aug;14(8):805-810. doi: 10.1038/nmeth.4335. Epub 2017 Jun 19. PMID:28628129[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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