4ogc: Difference between revisions

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 ==Crystal structure of the Type II-C Cas9 enzyme from Actinomyces naeslundii==
-<StructureSection load='4ogc' size='340' side='right' caption='[[4ogc]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='4ogc' size='340' side='right'caption='[[4ogc]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ogc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Actinomyces_naeslundii_'howell_279' Actinomyces naeslundii 'howell 279']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OGC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ogc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinomyces_naeslundii_str._Howell_279 Actinomyces naeslundii str. Howell 279]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OGC FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4oge|4oge]], [[4cmp|4cmp]], [[4cmq|4cmq]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ogc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogc OCA], [https://pdbe.org/4ogc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ogc RCSB], [https://www.ebi.ac.uk/pdbsum/4ogc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ogc ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HMPREF1129_2620 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1115803 Actinomyces naeslundii 'Howell 279'])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ogc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogc OCA], [http://pdbe.org/4ogc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ogc RCSB], [http://www.ebi.ac.uk/pdbsum/4ogc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ogc ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/CAS9_ACTNH CAS9_ACTNH] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity).<ref>PMID:24505130</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 ==See Also==
-*[[Cas9|Cas9]]
+*[[Endonuclease 3D structures|Endonuclease 3D structures]]
-*[[Endonuclease|Endonuclease]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Actinomyces naeslundii 'howell 279']]
+[[Category: Actinomyces naeslundii str. Howell 279]]
-[[Category: Doudna, J A]]
+[[Category: Large Structures]]
-[[Category: Jiang, F]]
+[[Category: Doudna JA]]
-[[Category: Lin, S]]
+[[Category: Jiang F]]
-[[Category: Ma, E]]
+[[Category: Lin S]]
-[[Category: Cas9]]
+[[Category: Ma E]]
-[[Category: Crispr-ca]]
-[[Category: Cytoplasmic]]
-[[Category: Hnh]]
-[[Category: Hydrolase]]
-[[Category: Rna-guided dna endonuclease]]
-[[Category: Ruvc]]