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==Crystal structure of human soluble Adenylyl Cyclase (Apo form)== | ==Crystal structure of human soluble Adenylyl Cyclase (Apo form)== | ||
<StructureSection load='4clf' size='340' side='right' caption='[[4clf]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='4clf' size='340' side='right'caption='[[4clf]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4clf]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4clf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CLF FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
<tr><td class="sblockLbl"><b>[[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4clf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4clf OCA], [https://pdbe.org/4clf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4clf RCSB], [https://www.ebi.ac.uk/pdbsum/4clf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4clf ProSAT]</span></td></tr> | |||
<tr | </table> | ||
= | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate.,Kleinboelting S, Diaz A, Moniot S, van den Heuvel J, Weyand M, Levin LR, Buck J, Steegborn C Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3727-32. doi:, 10.1073/pnas.1322778111. Epub 2014 Feb 24. PMID:24567411<ref>PMID:24567411</ref> | Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate.,Kleinboelting S, Diaz A, Moniot S, van den Heuvel J, Weyand M, Levin LR, Buck J, Steegborn C Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3727-32. doi:, 10.1073/pnas.1322778111. Epub 2014 Feb 24. PMID:24567411<ref>PMID:24567411</ref> | ||
From | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4clf" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[3D Adenylyl cyclase 3D structures|3D Adenylyl cyclase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Kleinboelting | [[Category: Kleinboelting S]] | ||
[[Category: Moniot | [[Category: Moniot S]] | ||
[[Category: Steegborn | [[Category: Steegborn C]] | ||
[[Category: Weyand | [[Category: Weyand M]] | ||
Latest revision as of 05:45, 21 November 2024
Crystal structure of human soluble Adenylyl Cyclase (Apo form)Crystal structure of human soluble Adenylyl Cyclase (Apo form)
Structural highlights
Publication Abstract from PubMedcAMP is an evolutionary conserved, prototypic second messenger regulating numerous cellular functions. In mammals, cAMP is synthesized by one of 10 homologous adenylyl cyclases (ACs): nine transmembrane enzymes and one soluble AC (sAC). Among these, only sAC is directly activated by bicarbonate (HCO3(-)); it thereby serves as a cellular sensor for HCO3(-), carbon dioxide (CO2), and pH in physiological functions, such as sperm activation, aqueous humor formation, and metabolic regulation. Here, we describe crystal structures of human sAC catalytic domains in the apo state and in complex with substrate analog, products, and regulators. The activator HCO3(-) binds adjacent to Arg176, which acts as a switch that enables formation of the catalytic cation sites. An anionic inhibitor, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid, inhibits sAC through binding to the active site entrance, which blocks HCO3(-) activation through steric hindrance and trapping of the Arg176 side chain. Finally, product complexes reveal small, local rearrangements that facilitate catalysis. Our results provide a molecular mechanism for sAC catalysis and cellular HCO3(-) sensing and a basis for targeting this system with drugs. Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate.,Kleinboelting S, Diaz A, Moniot S, van den Heuvel J, Weyand M, Levin LR, Buck J, Steegborn C Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3727-32. doi:, 10.1073/pnas.1322778111. Epub 2014 Feb 24. PMID:24567411[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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