4o9r: Difference between revisions

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<StructureSection load='4o9r' size='340' side='right'caption='[[4o9r]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='4o9r' size='340' side='right'caption='[[4o9r]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4o9r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O9R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O9R FirstGlance]. <br>
<table><tr><td colspan='2'>[[4o9r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O9R FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CY8:CYCLOPAMINE'>CY8</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.204&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o9r OCA], [http://pdbe.org/4o9r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o9r RCSB], [http://www.ebi.ac.uk/pdbsum/4o9r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o9r ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CY8:CYCLOPAMINE'>CY8</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o9r OCA], [https://pdbe.org/4o9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o9r RCSB], [https://www.ebi.ac.uk/pdbsum/4o9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o9r ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/SMO_HUMAN SMO_HUMAN]] G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.<ref>PMID:19592253</ref>
[https://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX] Electron-transport protein of unknown function.[https://www.uniprot.org/uniprot/SMO_HUMAN SMO_HUMAN] G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.<ref>PMID:19592253</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Escherichia coli]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Barty, A]]
[[Category: Barty A]]
[[Category: Basu, S]]
[[Category: Basu S]]
[[Category: Beyerlein, K R]]
[[Category: Beyerlein KR]]
[[Category: Boutet, S]]
[[Category: Boutet S]]
[[Category: Caffrey, M]]
[[Category: Caffrey M]]
[[Category: Chapman, H N]]
[[Category: Chapman HN]]
[[Category: Cherezov, V]]
[[Category: Cherezov V]]
[[Category: Doak, R B]]
[[Category: Doak RB]]
[[Category: Fromme, P]]
[[Category: Fromme P]]
[[Category: Fromme, R]]
[[Category: Fromme R]]
[[Category: GPCR, GPCR Network]]
[[Category: Gati C]]
[[Category: Gati, C]]
[[Category: Grotjohann I]]
[[Category: Grotjohann, I]]
[[Category: Han GW]]
[[Category: Han, G W]]
[[Category: Howe N]]
[[Category: Howe, N]]
[[Category: James D]]
[[Category: James, D]]
[[Category: Katritch V]]
[[Category: Katritch, V]]
[[Category: Kirian RA]]
[[Category: Kirian, R A]]
[[Category: Klinker M]]
[[Category: Klinker, M]]
[[Category: Koglin JE]]
[[Category: Koglin, J E]]
[[Category: Kupitz C]]
[[Category: Kupitz, C]]
[[Category: Li D]]
[[Category: Li, D]]
[[Category: Liu H]]
[[Category: Liu, H]]
[[Category: Liu W]]
[[Category: Liu, W]]
[[Category: Messerschmidt M]]
[[Category: Messerschmidt, M]]
[[Category: Nelson G]]
[[Category: Nelson, G]]
[[Category: Seibert MM]]
[[Category: Seibert, M M]]
[[Category: Shah STA]]
[[Category: Shah, S T.A]]
[[Category: Shoeman RL]]
[[Category: Shoeman, R L]]
[[Category: Spence JCH]]
[[Category: Spence, J C.H]]
[[Category: Stevens RC]]
[[Category: Stevens, R C]]
[[Category: Wacker D]]
[[Category: Wacker, D]]
[[Category: Wang C]]
[[Category: Wang, C]]
[[Category: Wang D]]
[[Category: Wang, D]]
[[Category: Weierstall U]]
[[Category: Weierstall, U]]
[[Category: White TA]]
[[Category: White, T A]]
[[Category: Willams GJ]]
[[Category: Willams, G J]]
[[Category: Zatsepin NA]]
[[Category: Zatsepin, N A]]
[[Category: Cyclopamine]]
[[Category: Free electron laser]]
[[Category: Gpcr]]
[[Category: Gpcr network]]
[[Category: Human smo receptor]]
[[Category: Lcp]]
[[Category: Membrane]]
[[Category: Membrane protein]]
[[Category: Novel protein engineering]]
[[Category: Psi-biology]]
[[Category: Room temperature structure]]
[[Category: Serial femtosecond crystallography]]
[[Category: Signaling protein]]
[[Category: Structural genomic]]
[[Category: Xfel]]

Latest revision as of 09:45, 17 October 2024

Human Smoothened Receptor structure in complex with cyclopamineHuman Smoothened Receptor structure in complex with cyclopamine

Structural highlights

4o9r is a 1 chain structure with sequence from Escherichia coli and Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.204Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C562_ECOLX Electron-transport protein of unknown function.SMO_HUMAN G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.[1]

Publication Abstract from PubMed

Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-mum-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.,Weierstall U, James D, Wang C, White TA, Wang D, Liu W, Spence JC, Bruce Doak R, Nelson G, Fromme P, Fromme R, Grotjohann I, Kupitz C, Zatsepin NA, Liu H, Basu S, Wacker D, Han GW, Katritch V, Boutet S, Messerschmidt M, Williams GJ, Koglin JE, Marvin Seibert M, Klinker M, Gati C, Shoeman RL, Barty A, Chapman HN, Kirian RA, Beyerlein KR, Stevens RC, Li D, Shah ST, Howe N, Caffrey M, Cherezov V Nat Commun. 2014;5:3309. doi: 10.1038/ncomms4309. PMID:24525480[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Endoh-Yamagami S, Evangelista M, Wilson D, Wen X, Theunissen JW, Phamluong K, Davis M, Scales SJ, Solloway MJ, de Sauvage FJ, Peterson AS. The mammalian Cos2 homolog Kif7 plays an essential role in modulating Hh signal transduction during development. Curr Biol. 2009 Aug 11;19(15):1320-6. doi: 10.1016/j.cub.2009.06.046. Epub 2009, Jul 9. PMID:19592253 doi:http://dx.doi.org/10.1016/j.cub.2009.06.046
  2. ↑ Weierstall U, James D, Wang C, White TA, Wang D, Liu W, Spence JC, Bruce Doak R, Nelson G, Fromme P, Fromme R, Grotjohann I, Kupitz C, Zatsepin NA, Liu H, Basu S, Wacker D, Han GW, Katritch V, Boutet S, Messerschmidt M, Williams GJ, Koglin JE, Marvin Seibert M, Klinker M, Gati C, Shoeman RL, Barty A, Chapman HN, Kirian RA, Beyerlein KR, Stevens RC, Li D, Shah ST, Howe N, Caffrey M, Cherezov V. Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography. Nat Commun. 2014;5:3309. doi: 10.1038/ncomms4309. PMID:24525480 doi:http://dx.doi.org/10.1038/ncomms4309

4o9r, resolution 3.20Å

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