4o9r: Difference between revisions

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-'''Unreleased structure'''
-The entry 4o9r is ON HOLD
+==Human Smoothened Receptor structure in complex with cyclopamine==
+<StructureSection load='4o9r' size='340' side='right'caption='[[4o9r]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4o9r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O9R FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.204&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CY8:CYCLOPAMINE'>CY8</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o9r OCA], [https://pdbe.org/4o9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o9r RCSB], [https://www.ebi.ac.uk/pdbsum/4o9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o9r ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX] Electron-transport protein of unknown function.[https://www.uniprot.org/uniprot/SMO_HUMAN SMO_HUMAN] G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.<ref>PMID:19592253</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-mum-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
-Authors: Wang, C., Weierstall, U., James, D., White, T.A., Wang, D. , Liu, W., Spence, J.C.H., Doak, R.B., Nelson, G., Fromme, P. , Fromme, R., Grotjohann, I., Kupitz, C., Zatsepin, N.A., Liu, H., Basu, S. , Wacker, D. , Han, G.W., Katritch, V. , Boutet, S. , Messerschmidt, M., Willams, G.J., Koglin, J.E., Seibert, M.M., Klinker, M. , Gati, C., Shoeman, R.L. , Barty, A., Chapman, H.N. , Kirian, R.A., Beyerlein, K.R., Stevens, R.C., Li, D. , Shah, S.T.A., Howe, N. , Caffrey, M. , Cherezov, V. , GPCR Network (GPCR)
+Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.,Weierstall U, James D, Wang C, White TA, Wang D, Liu W, Spence JC, Bruce Doak R, Nelson G, Fromme P, Fromme R, Grotjohann I, Kupitz C, Zatsepin NA, Liu H, Basu S, Wacker D, Han GW, Katritch V, Boutet S, Messerschmidt M, Williams GJ, Koglin JE, Marvin Seibert M, Klinker M, Gati C, Shoeman RL, Barty A, Chapman HN, Kirian RA, Beyerlein KR, Stevens RC, Li D, Shah ST, Howe N, Caffrey M, Cherezov V Nat Commun. 2014;5:3309. doi: 10.1038/ncomms4309. PMID:24525480<ref>PMID:24525480</ref>
-Description: Human Smoothened Receptor structure in complex with cyclopamine
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4o9r" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli]]
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Barty A]]
+[[Category: Basu S]]
+[[Category: Beyerlein KR]]
+[[Category: Boutet S]]
+[[Category: Caffrey M]]
+[[Category: Chapman HN]]
+[[Category: Cherezov V]]
+[[Category: Doak RB]]
+[[Category: Fromme P]]
+[[Category: Fromme R]]
+[[Category: Gati C]]
+[[Category: Grotjohann I]]
+[[Category: Han GW]]
+[[Category: Howe N]]
+[[Category: James D]]
+[[Category: Katritch V]]
+[[Category: Kirian RA]]
+[[Category: Klinker M]]
+[[Category: Koglin JE]]
+[[Category: Kupitz C]]
+[[Category: Li D]]
+[[Category: Liu H]]
+[[Category: Liu W]]
+[[Category: Messerschmidt M]]
+[[Category: Nelson G]]
+[[Category: Seibert MM]]
+[[Category: Shah STA]]
+[[Category: Shoeman RL]]
+[[Category: Spence JCH]]
+[[Category: Stevens RC]]
+[[Category: Wacker D]]
+[[Category: Wang C]]
+[[Category: Wang D]]
+[[Category: Weierstall U]]
+[[Category: White TA]]
+[[Category: Willams GJ]]
+[[Category: Zatsepin NA]]