Galactose-binding lectin: Difference between revisions
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<StructureSection load=' | <StructureSection load='' size='450' side='right' caption='Structure of peanut galactose-binding lectin tetramer complex with β-D-galactose, α-D-glucose, Ca+2 (green) and Mn+2 (purple) ions (PDB entry [[1v6i]])' scene='57/572274/Cv/1'> | ||
== Function == | == Function == | ||
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GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac. | GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac. | ||
</StructureSection> | |||
== Structural highlights == | |||
The carbohydrate ligand binds to 3 loops<ref>PMID:14747696</ref>. <scene name='57/572274/Cv/6'>β-D-galactose/α-D-glucose binding site</scene> in peanut galactose-binding lectin (PDB entry [[1v6i]]). | |||
<scene name='57/572274/Cv/7'>Ca coordination site</scene>. | |||
<scene name='57/572274/Cv/8'>Mn coordination site</scene>. Water molecules shown as red spheres. | |||
</StructureSection> | |||
== 3D structures of galactose-binding lectin == | == 3D structures of galactose-binding lectin == | ||
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**[[2dv9]], [[2dvd]] – GBL + β-D-galactose <br /> | **[[2dv9]], [[2dvd]] – GBL + β-D-galactose <br /> | ||
**[[1rir]] – GBL + porphyrin derivative <br /> | **[[1rir]] – GBL + porphyrin derivative <br /> | ||
**[[6v95]], [[6vav]], [[6vaw]], [[6vc3]], [[6vc4]], [[6vgf]] – GBL + galactopyranose derivative <br /> | |||
*''Peanut GBL ternary complex'' | *''Peanut GBL ternary complex'' | ||
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**[[1l7l]], [[1uoj]] – GBL <br /> | **[[1l7l]], [[1uoj]] – GBL <br /> | ||
**[[4cp9]], [[4cpb]], [[7z62]], [[7z63]] – GBL + galactoside <br /> | |||
**[[7fio]], [[7fjh]] – GBL + inhibitor<br /> | |||
*''PaGBL binary complex'' | *''PaGBL binary complex'' | ||
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**[[2wyf]] – GBL + methyl-β-galactose <br /> | **[[2wyf]] – GBL + methyl-β-galactose <br /> | ||
**[[4ljh]] – GBL + β-D-galactose <br /> | **[[4ljh]] – GBL + β-D-galactose <br /> | ||
**[[3zyf]], [[4a6s]] – GBL + galactopyranose derivative<br /> | **[[3zyf]], [[4a6s]], [[4yw6]], [[5mih]] – GBL + galactopyranose derivative<br /> | ||
**[[4ywa]] – GBL + triazole derivative <br /> | |||
**[[6yo3]], [[6yoh]] – GBL + oxidanyl derivative <br /> | |||
*''PaGBL ternary complex'' | *''PaGBL ternary complex'' | ||
**[[3zyh]] – GBL + galactopyranose derivative + gal-lys-pro-leu <br /> | **[[3zyh]] – GBL + galactopyranose derivative + gal-lys-pro-leu <br /> | ||
**[[4yw7]] – GBL + galactopyranose derivative + triazole derivative <br /> | |||
**[[3zyb]] – GBL + β-D-galactose + gal-lys-pro-leu<br /> | **[[3zyb]] – GBL + β-D-galactose + gal-lys-pro-leu<br /> | ||
**[[4lk6]] – GBL + β-D-galactose + chlorophenol red<br /> | **[[4lk6]] – GBL + β-D-galactose + chlorophenol red<br /> | ||
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**[[2ds0]] – LtGBL C terminal (mutant) + sialyllactose <br /> | **[[2ds0]] – LtGBL C terminal (mutant) + sialyllactose <br /> | ||
**[[2zqo]] – LtGBL C terminal + GalNac <br /> | **[[2zqo]] – LtGBL C terminal + GalNac <br /> | ||
*Other GBL | |||
**[[5x4a]], [[3wmp]], [[3wmq]] – GBL residues 47-140 + polysaccharide – ''Sinularia lochmodes'' <br /> | |||
**[[2zqn]] – eGBL C-terminal + lactose – earthworm<br /> | |||
**[[2zqo]] – eGBL C-terminal + GalNac<br /> | |||
**[[6yf6]] – GBL C-terminal + fucoside derivative – ''Enterobacter cloacae''<br /> | |||
**[[5vbk]], [[8vck]] – GBL - mussell<br /> | |||
}} | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Latest revision as of 11:37, 29 January 2025
FunctionGalactose-binding lectin (GBL) is a lectin which binds the carbohydrate sequence Gal-β(1,3)-GalNac. GBL is an all beta-sheet protein[1]. RelevanceGBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac. Structural highlightsThe carbohydrate ligand binds to 3 loops[2]. in peanut galactose-binding lectin (PDB entry 1v6i). . . Water molecules shown as red spheres. |
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3D structures of galactose-binding lectin3D structures of galactose-binding lectin
Updated on 29-January-2025
ReferencesReferences
- ↑ Natchiar SK, Srinivas O, Mitra N, Surolia A, Jayaraman N, Vijayan M. Structural studies on peanut lectin complexed with disaccharides involving different linkages: further insights into the structure and interactions of the lectin. Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1413-21. Epub 2006, Oct 18. PMID:17057347 doi:10.1107/S0907444906035712
- ↑ Kundhavai Natchiar S, Arockia Jeyaprakash A, Ramya TN, Thomas CJ, Suguna K, Surolia A, Vijayan M. Structural plasticity of peanut lectin: an X-ray analysis involving variation in pH, ligand binding and crystal structure. Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):211-9. Epub 2004, Jan 23. PMID:14747696 doi:http://dx.doi.org/10.1107/S090744490302849X