Galactose-binding lectin: Difference between revisions
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<StructureSection load=' | <StructureSection load='' size='450' side='right' caption='Structure of peanut galactose-binding lectin tetramer complex with β-D-galactose, α-D-glucose, Ca+2 (green) and Mn+2 (purple) ions (PDB entry [[1v6i]])' scene='57/572274/Cv/1'> | ||
== Function == | |||
'''Galactose-binding lectin''' (GBL) is a lectin which binds the carbohydrate sequence Gal-β(1,3)-GalNac. GBL is an all beta-sheet protein. GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac. | '''Galactose-binding lectin''' (GBL) is a lectin which binds the carbohydrate sequence Gal-β(1,3)-GalNac. GBL is an all beta-sheet protein<ref>PMID:17057347</ref>. | ||
== Relevance == | |||
GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac. | |||
== Structural highlights == | |||
The carbohydrate ligand binds to 3 loops<ref>PMID:14747696</ref>. <scene name='57/572274/Cv/6'>β-D-galactose/α-D-glucose binding site</scene> in peanut galactose-binding lectin (PDB entry [[1v6i]]). | |||
<scene name='57/572274/Cv/7'>Ca coordination site</scene>. | |||
<scene name='57/572274/Cv/8'>Mn coordination site</scene>. Water molecules shown as red spheres. | |||
</StructureSection> | |||
== 3D structures of galactose-binding lectin == | == 3D structures of galactose-binding lectin == | ||
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**[[2dv9]], [[2dvd]] – GBL + β-D-galactose <br /> | **[[2dv9]], [[2dvd]] – GBL + β-D-galactose <br /> | ||
**[[1rir]] – GBL + porphyrin derivative <br /> | **[[1rir]] – GBL + porphyrin derivative <br /> | ||
**[[6v95]], [[6vav]], [[6vaw]], [[6vc3]], [[6vc4]], [[6vgf]] – GBL + galactopyranose derivative <br /> | |||
*''Peanut GBL ternary complex'' | *''Peanut GBL ternary complex'' | ||
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**[[1l7l]], [[1uoj]] – GBL <br /> | **[[1l7l]], [[1uoj]] – GBL <br /> | ||
**[[4cp9]], [[4cpb]], [[7z62]], [[7z63]] – GBL + galactoside <br /> | |||
**[[7fio]], [[7fjh]] – GBL + inhibitor<br /> | |||
*'' | *''PaGBL binary complex'' | ||
**[[2wyf]] – GBL + methyl-β-galactose <br /> | **[[2wyf]] – GBL + methyl-β-galactose <br /> | ||
**[[4ljh]] – GBL + β-D-galactose <br /> | **[[4ljh]] – GBL + β-D-galactose <br /> | ||
**[[3zyf]], [[4a6s]] – GBL + galactopyranose derivative<br /> | **[[3zyf]], [[4a6s]], [[4yw6]], [[5mih]] – GBL + galactopyranose derivative<br /> | ||
**[[4ywa]] – GBL + triazole derivative <br /> | |||
**[[6yo3]], [[6yoh]] – GBL + oxidanyl derivative <br /> | |||
*'' | *''PaGBL ternary complex'' | ||
**[[3zyh]] – GBL + galactopyranose derivative + gal-lys-pro-leu <br /> | **[[3zyh]] – GBL + galactopyranose derivative + gal-lys-pro-leu <br /> | ||
**[[4yw7]] – GBL + galactopyranose derivative + triazole derivative <br /> | |||
**[[3zyb]] – GBL + β-D-galactose + gal-lys-pro-leu<br /> | **[[3zyb]] – GBL + β-D-galactose + gal-lys-pro-leu<br /> | ||
**[[4lk6]] – GBL + β-D-galactose + chlorophenol red<br /> | **[[4lk6]] – GBL + β-D-galactose + chlorophenol red<br /> | ||
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**[[4al9]] – GBL + α-D-galactose + α-D-glucose <br /> | **[[4al9]] – GBL + α-D-galactose + α-D-glucose <br /> | ||
*'' | *''PaGBL quaternary complex'' | ||
**[[2vxj]] – GBL + β-D-galactose + α-D-galactose + α-D-glucose + β-D-glucose<br /> | **[[2vxj]] – GBL + β-D-galactose + α-D-galactose + α-D-glucose + β-D-glucose<br /> | ||
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**[[2ds0]] – LtGBL C terminal (mutant) + sialyllactose <br /> | **[[2ds0]] – LtGBL C terminal (mutant) + sialyllactose <br /> | ||
**[[2zqo]] – LtGBL C terminal + GalNac <br /> | **[[2zqo]] – LtGBL C terminal + GalNac <br /> | ||
*Other GBL | |||
**[[5x4a]], [[3wmp]], [[3wmq]] – GBL residues 47-140 + polysaccharide – ''Sinularia lochmodes'' <br /> | |||
**[[2zqn]] – eGBL C-terminal + lactose – earthworm<br /> | |||
**[[2zqo]] – eGBL C-terminal + GalNac<br /> | |||
**[[6yf6]] – GBL C-terminal + fucoside derivative – ''Enterobacter cloacae''<br /> | |||
**[[5vbk]], [[8vck]] – GBL - mussell<br /> | |||
}} | }} | ||
== References == | |||
<references/> | |||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||