Galactose-binding lectin: Difference between revisions

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<StructureSection load='1v6i' size='350' side='right' caption='Structure of peanut galactose-binding lectin tetramer complex with β-D-galactose, α-D-glucose, Ca+2 (green) and Mn+2 (purple) ions (PDB entry [[1v6i]])' scene=''>
<StructureSection load='' size='450' side='right' caption='Structure of peanut galactose-binding lectin tetramer complex with β-D-galactose, α-D-glucose, Ca+2 (green) and Mn+2 (purple) ions (PDB entry [[1v6i]])' scene='57/572274/Cv/1'>
 
== Function ==
    
    
'''Galactose-binding lectin''' (GBL) is a lectin which binds the carbohydrate sequence Gal-β(1,3)-GalNac.  GBL is an all beta-sheet protein.  GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac.
'''Galactose-binding lectin''' (GBL) is a lectin which binds the carbohydrate sequence Gal-β(1,3)-GalNac.  GBL is an all beta-sheet protein<ref>PMID:17057347</ref>.   
 
== Relevance ==
 
GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac.
 
== Structural highlights ==
 
The carbohydrate ligand binds to 3 loops<ref>PMID:14747696</ref>. <scene name='57/572274/Cv/6'>β-D-galactose/α-D-glucose binding site</scene> in peanut galactose-binding lectin (PDB entry [[1v6i]]).
 
<scene name='57/572274/Cv/7'>Ca coordination site</scene>.
 
<scene name='57/572274/Cv/8'>Mn coordination site</scene>. Water molecules shown as red spheres.
</StructureSection>


== 3D structures of galactose-binding lectin ==
== 3D structures of galactose-binding lectin ==


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
{{#tree:id=OrganizedByTopic|openlevels=0|
*Peanut GBL
**[[1cq9]], [[1v6m]], [[1v6n]], [[1v6o]], [[2dh1]], [[2dvf]] – GBL <br />
*''Peanut GBL binary complex''


'''Peanut GBL'''
**[[1qf3]] – GBL + methyl-β-galactose <br />
**[[2dv9]], [[2dvd]] – GBL + β-D-galactose <br />
**[[1rir]] – GBL + porphyrin derivative <br />
**[[6v95]], [[6vav]], [[6vaw]], [[6vc3]], [[6vc4]], [[6vgf]] – GBL + galactopyranose derivative <br />


[[1cq9]], [[1v6m]], [[1v6n]], [[1v6o]], [[2dh1]], [[2dvf]] – GBL <br />
*''Peanut GBL ternary complex''


''Peanut GBL binary complex''
**[[1v6i]], [[1v6j]], [[1v6k]], [[1cr7]], [[2dvg]] – GBL + β-D-galactose + α-D-glucose <br />
**[[2pel]] – GBL + β-lactose + α-lactose <br />
**[[2tep]], [[2dva]], [[2dvb]] – GBL + β-D-galactose + N-acetyl-D-galactosamine <br />
**[[1ciw]] – GBL + β-D-galactose + 2-acetylamino-2-deoxy-D-glucopyranose <br />
**[[1rit]] – GBL + porphyrin derivative + β-lactose<br />


[[1qf3]] – GBL + methyl-β-galactose <br />
*''Peanut GBL quaternary complex''
[[2dv9]], [[2dvd]] – GBL + β-D-galactose <br />
[[1rir]] – GBL + porphyrin derivative <br />


''Peanut GBL ternary complex''
**[[1bzw]], [[1v6l]] – GBL + β-D-galactose + α-D-glucose + β-D-glucose<br />


[[1v6i]], [[1v6j]], [[1v6k]], [[1cr7]], [[2dvg]] – GBL + β-D-galactose + α-D-glucose <br />
*''Pseudomonas aeruginosa'' GBL
[[2pel]] – GBL + β-lactose + α-lactose <br />
[[2tep]], [[2dva]], [[2dvb]] – GBL + β-D-galactose + N-acetyl-D-galactosamine <br />
[[1ciw]] – GBL + β-D-galactose + 2-acetylamino-2-deoxy-D-glucopyranose <br />
[[1rit]] – GBL + porphyrin derivative + β-lactose<br />


''Peanut GBL quaternary complex''
**[[1l7l]], [[1uoj]] – GBL <br />
**[[4cp9]], [[4cpb]], [[7z62]], [[7z63]] – GBL + galactoside <br />
**[[7fio]], [[7fjh]] – GBL + inhibitor<br />


[[1bzw]], [[1v6l]] – GBL + β-D-galactose + α-D-glucose + β-D-glucose<br />
*''PaGBL binary complex''


'''''Pseudomonas aeruginosa'' GBL'''
**[[2wyf]] – GBL + methyl-β-galactose <br />
**[[4ljh]] – GBL + β-D-galactose <br />
**[[3zyf]], [[4a6s]], [[4yw6]], [[5mih]] – GBL + galactopyranose derivative<br />
**[[4ywa]] – GBL + triazole derivative <br />
**[[6yo3]], [[6yoh]] – GBL + oxidanyl derivative <br />


[[1l7l]], [[1uoj]] – GBL <br />
*''PaGBL ternary complex''


''PsGBL binary complex''
**[[3zyh]] – GBL + galactopyranose derivative + gal-lys-pro-leu <br />
**[[4yw7]] – GBL + galactopyranose derivative + triazole derivative <br />
**[[3zyb]] – GBL + β-D-galactose + gal-lys-pro-leu<br />
**[[4lk6]] – GBL + β-D-galactose + chlorophenol red<br />
**[[4lk7]] – GBL + β-D-galactose + resorufin<br />
**[[1oko]] – GBL + β-D-galactose + α-D-galactose <br />
**[[4al9]] – GBL + α-D-galactose + α-D-glucose <br />


[[2wyf]] – GBL + methyl-β-galactose <br />
*''PaGBL quaternary complex''
[[4ljh]] – GBL + β-D-galactose <br />
[[3zyf]], [[4a6s]] – GBL + galactopyranose derivative<br />


''PsGBL ternary complex''
**[[2vxj]] – GBL + β-D-galactose + α-D-galactose + α-D-glucose + β-D-glucose<br />


[[3zyh]] – GBL + galactopyranose derivative + gal-lys-pro-leu <br />
*''Lumbricus terrestris'' GBL
[[3zyb]] – GBL + β-D-galactose + gal-lys-pro-leu<br />
[[4lk6]] – GBL + β-D-galactose + chlorophenol red<br />
[[4lk7]] – GBL + β-D-galactose + resorufin<br />
[[1oko]] – GBL + β-D-galactose + α-D-galactose <br />
[[4al9]] – GBL + α-D-galactose + α-D-glucose <br />


''PsGBL quaternary complex''
**[[2dry]] – LtGBL C terminal (mutant) – ''Lumbricus terrestris''<br />
**[[2rst]] – LtGBL C terminal <br />
**[[2drz]] – LtGBL C terminal (mutant) + lactose <br />
**[[2zqn]] – LtGBL C terminal + lactose <br />
**[[2ds0]] – LtGBL C terminal (mutant) + sialyllactose <br />
**[[2zqo]] – LtGBL C terminal + GalNac <br />


[[2vxj]] – GBL + β-D-galactose + α-D-galactose + α-D-glucose + β-D-glucose<br />
*Other GBL


**[[5x4a]], [[3wmp]], [[3wmq]] – GBL residues 47-140 + polysaccharide – ''Sinularia lochmodes'' <br />
**[[2zqn]] – eGBL C-terminal + lactose – earthworm<br />
**[[2zqo]] – eGBL C-terminal + GalNac<br />
**[[6yf6]] – GBL C-terminal + fucoside derivative – ''Enterobacter cloacae''<br />
**[[5vbk]], [[8vck]] – GBL - mussell<br />
}}
== References ==
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 11:37, 29 January 2025


Function

Galactose-binding lectin (GBL) is a lectin which binds the carbohydrate sequence Gal-β(1,3)-GalNac. GBL is an all beta-sheet protein[1].

Relevance

GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac.

Structural highlights

The carbohydrate ligand binds to 3 loops[2]. in peanut galactose-binding lectin (PDB entry 1v6i).

.

. Water molecules shown as red spheres.

Structure of peanut galactose-binding lectin tetramer complex with β-D-galactose, α-D-glucose, Ca+2 (green) and Mn+2 (purple) ions (PDB entry 1v6i)

Drag the structure with the mouse to rotate

3D structures of galactose-binding lectin3D structures of galactose-binding lectin

Updated on 29-January-2025

ReferencesReferences

  1. ↑ Natchiar SK, Srinivas O, Mitra N, Surolia A, Jayaraman N, Vijayan M. Structural studies on peanut lectin complexed with disaccharides involving different linkages: further insights into the structure and interactions of the lectin. Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1413-21. Epub 2006, Oct 18. PMID:17057347 doi:10.1107/S0907444906035712
  2. ↑ Kundhavai Natchiar S, Arockia Jeyaprakash A, Ramya TN, Thomas CJ, Suguna K, Surolia A, Vijayan M. Structural plasticity of peanut lectin: an X-ray analysis involving variation in pH, ligand binding and crystal structure. Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):211-9. Epub 2004, Jan 23. PMID:14747696 doi:http://dx.doi.org/10.1107/S090744490302849X

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