Galactose-binding lectin: Difference between revisions

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Michal Harel, Alexander Berchansky, Joel L. Sussman, Jaime Prilusky

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-<StructureSection load='1v6i' size='350' side='right' caption='Structure of peanut galactose-binding lectin tetramer complex with β-D-galactose, α-D-glucose, Ca+2 (green) and Mn+2 (purple) ions (PDB entry [[1v6i]])' scene=''>
+<StructureSection load='' size='450' side='right' caption='Structure of peanut galactose-binding lectin tetramer complex with β-D-galactose, α-D-glucose, Ca+2 (green) and Mn+2 (purple) ions (PDB entry [[1v6i]])' scene='57/572274/Cv/1'>
+== Function ==
-'''Galactose-binding lectin''' (GBL) is a lectin which binds the carbohydrate sequence Gal-β(1,3)-GalNac.  GBL is an all beta-sheet protein.  GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac.
+'''Galactose-binding lectin''' (GBL) is a lectin which binds the carbohydrate sequence Gal-β(1,3)-GalNac.  GBL is an all beta-sheet protein<ref>PMID:17057347</ref>.
+== Relevance ==
+GBL is used in affinity columns for capturing proteins which are glycosylated with Gal-β(1,3)-GalNac.
+== Structural highlights ==
+The carbohydrate ligand binds to 3 loops<ref>PMID:14747696</ref>. <scene name='57/572274/Cv/6'>β-D-galactose/α-D-glucose binding site</scene> in peanut galactose-binding lectin (PDB entry [[1v6i]]).
+<scene name='57/572274/Cv/7'>Ca coordination site</scene>.
+<scene name='57/572274/Cv/8'>Mn coordination site</scene>. Water molecules shown as red spheres.
+</StructureSection>
 == 3D structures of galactose-binding lectin ==
 Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+{{#tree:id=OrganizedByTopic|openlevels=0|
+*Peanut GBL
+**[[1cq9]], [[1v6m]], [[1v6n]], [[1v6o]], [[2dh1]], [[2dvf]] – GBL <br />
+*''Peanut GBL binary complex''
+**[[1qf3]] – GBL + methyl-β-galactose <br />
+**[[2dv9]], [[2dvd]] – GBL + β-D-galactose <br />
+**[[1rir]] – GBL + porphyrin derivative <br />
+**[[6v95]], [[6vav]], [[6vaw]], [[6vc3]], [[6vc4]], [[6vgf]] – GBL + galactopyranose derivative <br />
-'''Peanut GBL'''
+*''Peanut GBL ternary complex''
-[[1cq9]], [[1v6m]], [[1v6n]], [[1v6o]], [[2dh1]], [[2dvf]] – GBL <br />
+**[[1v6i]], [[1v6j]], [[1v6k]], [[1cr7]], [[2dvg]] – GBL + β-D-galactose + α-D-glucose <br />
+**[[2pel]] – GBL + β-lactose + α-lactose <br />
+**[[2tep]], [[2dva]], [[2dvb]] – GBL + β-D-galactose + N-acetyl-D-galactosamine <br />
+**[[1ciw]] – GBL + β-D-galactose + 2-acetylamino-2-deoxy-D-glucopyranose <br />
+**[[1rit]] – GBL + porphyrin derivative + β-lactose<br />
-''Peanut GBL binary complex''
+*''Peanut GBL quaternary complex''
-[[1qf3]] – GBL + methyl-β-galactose <br />
+**[[1bzw]], [[1v6l]] – GBL + β-D-galactose + α-D-glucose + β-D-glucose<br />
-[[2dv9]], [[2dvd]] – GBL + β-D-galactose <br />
-[[1rir]] – GBL + porphyrin derivative <br />
-''Peanut GBL ternary complex''
+*''Pseudomonas aeruginosa'' GBL
-[[1v6i]], [[1v6j]], [[1v6k]], [[1cr7]], [[2dvg]] – GBL + β-D-galactose + α-D-glucose <br />
+**[[1l7l]], [[1uoj]] – GBL <br />
-[[2pel]] – GBL + β-lactose + α-lactose <br />
+**[[4cp9]], [[4cpb]], [[7z62]], [[7z63]] – GBL + galactoside <br />
-[[2tep]], [[2dva]], [[2dvb]] – GBL + β-D-galactose + N-acetyl-D-galactosamine <br />
+**[[7fio]], [[7fjh]] – GBL + inhibitor<br />
-[[1ciw]] – GBL + β-D-galactose + 2-acetylamino-2-deoxy-D-glucopyranose <br />
-[[1rit]] – GBL + porphyrin derivative + β-lactose<br />
-''Peanut GBL quaternary complex''
+*''PaGBL binary complex''
-[[1bzw]], [[1v6l]] – GBL + β-D-galactose + α-D-glucose + β-D-glucose<br />
+**[[2wyf]] – GBL + methyl-β-galactose <br />
+**[[4ljh]] – GBL + β-D-galactose <br />
+**[[3zyf]], [[4a6s]], [[4yw6]], [[5mih]] – GBL + galactopyranose derivative<br />
+**[[4ywa]] – GBL + triazole derivative <br />
+**[[6yo3]], [[6yoh]] – GBL + oxidanyl derivative <br />
-'''''Pseudomonas aeruginosa'' GBL'''
+*''PaGBL ternary complex''
-[[1l7l]], [[1uoj]] – GBL <br />
+**[[3zyh]] – GBL + galactopyranose derivative + gal-lys-pro-leu <br />
+**[[4yw7]] – GBL + galactopyranose derivative + triazole derivative <br />
+**[[3zyb]] – GBL + β-D-galactose + gal-lys-pro-leu<br />
+**[[4lk6]] – GBL + β-D-galactose + chlorophenol red<br />
+**[[4lk7]] – GBL + β-D-galactose + resorufin<br />
+**[[1oko]] – GBL + β-D-galactose + α-D-galactose <br />
+**[[4al9]] – GBL + α-D-galactose + α-D-glucose <br />
-''PsGBL binary complex''
+*''PaGBL quaternary complex''
-[[2wyf]] – GBL + methyl-β-galactose <br />
+**[[2vxj]] – GBL + β-D-galactose + α-D-galactose + α-D-glucose + β-D-glucose<br />
-[[4ljh]] – GBL + β-D-galactose <br />
-[[3zyf]], [[4a6s]] – GBL + galactopyranose derivative<br />
-''PsGBL ternary complex''
+*''Lumbricus terrestris'' GBL
-[[3zyh]] – GBL + galactopyranose derivative + gal-lys-pro-leu <br />
+**[[2dry]] – LtGBL C terminal (mutant) – ''Lumbricus terrestris''<br />
-[[3zyb]] – GBL + β-D-galactose + gal-lys-pro-leu<br />
+**[[2rst]] – LtGBL C terminal <br />
-[[4lk6]] – GBL + β-D-galactose + chlorophenol red<br />
+**[[2drz]] – LtGBL C terminal (mutant) + lactose <br />
-[[4lk7]] – GBL + β-D-galactose + resorufin<br />
+**[[2zqn]] – LtGBL C terminal + lactose <br />
-[[1oko]] – GBL + β-D-galactose + α-D-galactose <br />
+**[[2ds0]] – LtGBL C terminal (mutant) + sialyllactose <br />
-[[4al9]] – GBL + α-D-galactose + α-D-glucose <br />
+**[[2zqo]] – LtGBL C terminal + GalNac <br />
-''PsGBL quaternary complex''
+*Other GBL
-[[2vxj]] – GBL + β-D-galactose + α-D-galactose + α-D-glucose + β-D-glucose<br />
+**[[5x4a]], [[3wmp]], [[3wmq]] – GBL residues 47-140 + polysaccharide – ''Sinularia lochmodes'' <br />
+**[[2zqn]] – eGBL C-terminal + lactose – earthworm<br />
+**[[2zqo]] – eGBL C-terminal + GalNac<br />
+**[[6yf6]] – GBL C-terminal + fucoside derivative – ''Enterobacter cloacae''<br />
+**[[5vbk]], [[8vck]] – GBL - mussell<br />
+}}
+== References ==
+<references/>
+[[Category:Topic Page]]