4n1d: Difference between revisions
No edit summary |
No edit summary |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Nodal/BMP2 chimera NB250== | ==Nodal/BMP2 chimera NB250== | ||
<StructureSection load='4n1d' size='340' side='right' caption='[[4n1d]], [[Resolution|resolution]] 1.91Å' scene=''> | <StructureSection load='4n1d' size='340' side='right'caption='[[4n1d]], [[Resolution|resolution]] 1.91Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4n1d]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4n1d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N1D FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.912Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n1d OCA], [https://pdbe.org/4n1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n1d RCSB], [https://www.ebi.ac.uk/pdbsum/4n1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n1d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Disease == | |||
[https://www.uniprot.org/uniprot/NODAL_HUMAN NODAL_HUMAN] Alobar holoprosencephaly;Situs ambiguus;Lobar holoprosencephaly;Semilobar holoprosencephaly;Midline interhemispheric variant of holoprosencephaly;Septopreoptic holoprosencephaly;Situs inversus totalis;Microform holoprosencephaly. The disease is caused by variants affecting the gene represented in this entry. | |||
== Function == | == Function == | ||
[[ | [https://www.uniprot.org/uniprot/NODAL_HUMAN NODAL_HUMAN] Essential for mesoderm formation and axial patterning during embryonic development.[https://www.uniprot.org/uniprot/BMP2_HUMAN BMP2_HUMAN] Induces cartilage and bone formation. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 16: | Line 19: | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4n1d" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Bone morphogenetic protein 3D structures|Bone morphogenetic protein 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Esquivies L]] | ||
Latest revision as of 13:18, 30 October 2024
Nodal/BMP2 chimera NB250Nodal/BMP2 chimera NB250
Structural highlights
DiseaseNODAL_HUMAN Alobar holoprosencephaly;Situs ambiguus;Lobar holoprosencephaly;Semilobar holoprosencephaly;Midline interhemispheric variant of holoprosencephaly;Septopreoptic holoprosencephaly;Situs inversus totalis;Microform holoprosencephaly. The disease is caused by variants affecting the gene represented in this entry. FunctionNODAL_HUMAN Essential for mesoderm formation and axial patterning during embryonic development.BMP2_HUMAN Induces cartilage and bone formation. Publication Abstract from PubMedNodal, a member of the TGF-beta superfamily, plays an important role in vertebrate and invertebrate early development. The biochemical study of Nodal and its signaling pathway has been a challenge, mainly because of difficulties in producing the protein in sufficient quantities. We have developed a library of stable, chemically refoldable Nodal/BMP2 chimeric ligands (NB2 library). Three chimeras, named NB250, NB260, and NB264, show Nodal-like signaling properties including dependence on the co-receptor Cripto and activation of the Smad2 pathway. NB250, like Nodal, alters heart looping during the establishment of embryonic left-right asymmetry, and both NB250 and NB260, as well as Nodal, induce chondrogenic differentiation of human adipose-derived stem cells. This Nodal-induced differentiation is shown to be more efficient than BPM2-induced differentiation. Interestingly, the crystal structure of NB250 shows a backbone scaffold similar to that of BMP2. Our results show that these chimeric ligands may have therapeutic implications in cartilage injuries. Designer Nodal/BMP2 Chimeras Mimic Nodal Signaling, Promote Chondrogenesis, and Reveal a BMP2-like Structure.,Esquivies L, Blackler A, Peran M, Rodriguez-Esteban C, Izpisua Belmonte JC, Booker E, Gray PC, Ahn C, Kwiatkowski W, Choe S J Biol Chem. 2014 Jan 17;289(3):1788-97. doi: 10.1074/jbc.M113.529180. Epub 2013 , Dec 5. PMID:24311780[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||