4by9: Difference between revisions

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-{{STRUCTURE_4by9|  PDB=4by9  |  SCENE=  }}
-===The structure of the Box CD enzyme reveals regulation of rRNA methylation===
-{{ABSTRACT_PUBMED_24121435}}
-==Function==
+==The structure of the Box CD enzyme reveals regulation of rRNA methylation==
-[[http://www.uniprot.org/uniprot/FLPA_PYRFU FLPA_PYRFU]] Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity).[HAMAP-Rule:MF_00351] [[http://www.uniprot.org/uniprot/RL7A_PYRFU RL7A_PYRFU]] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA, box C/D and box C'/D' sRNAs (By similarity).
+<StructureSection load='4by9' size='340' side='right'caption='[[4by9]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4by9]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BY9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4by9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4by9 OCA], [https://pdbe.org/4by9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4by9 RCSB], [https://www.ebi.ac.uk/pdbsum/4by9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4by9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8U4M1_PYRFU Q8U4M1_PYRFU]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Post-transcriptional modifications are essential to the cell life cycle, as they affect both pre-ribosomal RNA processing and ribosome assembly. The box C/D ribonucleoprotein enzyme that methylates ribosomal RNA at the 2'-O-ribose uses a multitude of guide RNAs as templates for the recognition of rRNA target sites. Two methylation guide sequences are combined on each guide RNA, the significance of which has remained unclear. Here we use a powerful combination of NMR spectroscopy and small-angle neutron scattering to solve the structure of the 390 kDa archaeal RNP enzyme bound to substrate RNA. We show that the two methylation guide sequences are located in different environments in the complex and that the methylation of physiological substrates targeted by the same guide RNA occurs sequentially. This structure provides a means for differential control of methylation levels at the two sites and at the same time offers an unexpected regulatory mechanism for rRNA folding.
-==About this Structure==
+The structure of the box C/D enzyme reveals regulation of RNA methylation.,Lapinaite A, Simon B, Skjaerven L, Rakwalska-Bange M, Gabel F, Carlomagno T Nature. 2013 Oct 13. doi: 10.1038/nature12581. PMID:24121435<ref>PMID:24121435</ref>
-[[4by9]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BY9 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:024121435</ref><references group="xtra"/><references/>
+</div>
+<div class="pdbe-citations 4by9" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Pyrococcus furiosus]]
-[[Category: Carlomagno, T.]]
+[[Category: Carlomagno T]]
-[[Category: Gabel, F.]]
+[[Category: Gabel F]]
-[[Category: Lapinaite, A.]]
+[[Category: Lapinaite A]]
-[[Category: Rakwalska-Bange, M.]]
+[[Category: Rakwalska-Bange M]]
-[[Category: Simon, B.]]
+[[Category: Simon B]]
-[[Category: Skjaerven, L.]]
+[[Category: Skjaerven L]]
-[[Category: Rrna modification]]
-[[Category: Transferase-rna complex]]