4jno: Difference between revisions
New page: {{STRUCTURE_4jno| PDB=4jno | SCENE= }} ===Crystal structure of Plasmodium falciparum Erythrocyte Binding Antigen 140 (PfEBA-140/BAEBL) Region II in complex with sialyllactose=== ==Ab... |
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== | ==Crystal structure of Plasmodium falciparum Erythrocyte Binding Antigen 140 (PfEBA-140/BAEBL) Region II in complex with sialyllactose== | ||
[[4jno]] is a 1 chain structure with sequence from [ | <StructureSection load='4jno' size='340' side='right'caption='[[4jno]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4jno]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JNO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jno OCA], [https://pdbe.org/4jno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jno RCSB], [https://www.ebi.ac.uk/pdbsum/4jno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jno ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8WS31_PLAFA Q8WS31_PLAFA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Plasmodium falciparum erythrocyte invasion is dependent on high-affinity recognition of sialic acid on cell-surface receptors. The Erythrocyte Binding-like (EBL) family of invasion ligands mediates recognition of sialic acid on erythrocyte glycoproteins. Erythrocyte Binding Antigen-140 (PfEBA-140/BAEBL) is a critical EBL ligand that binds sialic acid on its receptor Glycophorin C. We present here the crystal structure of the two domain receptor-binding region of PfEBA-140 in complex with a glycan containing sialic acid. The structure identifies two glycan-binding pockets unique to PfEBA-140 and not shared by other EBL ligands. Specific molecular interactions that enable receptor engagement are identified and reveal that the glycan-binding mode is distinct from that of Apicomplexan and viral cell surface recognition ligands as well as host immune factors that bind sialic acid. Erythrocyte binding experiments elucidated essential glycan contact residues and identified divergent functional roles for each receptor binding site. One of four polymorphisms proposed to affect receptor-binding was localized to a glycan binding site, providing a structural basis for altered erythrocyte engagement. The studies described here provide the first full description of sialic acid-dependent molecular interactions at the P. falciparum erythrocyte invasion interface and define a framework for development of PfEBA-140 based therapeutics, vaccines, and diagnostics assessing vaccine efficacy and natural immunity to infection. | |||
Molecular basis for sialic acid-dependent receptor recognition by Plasmodium falciparum erythrocyte binding antigen 140/BAEBL.,Malpede BM, Lin DH, Tolia NH J Biol Chem. 2013 Mar 18. PMID:23508963<ref>PMID:23508963</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4jno" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Erythrocyte binding antigen|Erythrocyte binding antigen]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Plasmodium falciparum]] | [[Category: Plasmodium falciparum]] | ||
[[Category: Tolia | [[Category: Tolia NH]] | ||