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==Crystal Structure of Phosphorylated C.elegans Thymidylate Synthase in Complex with dUMP== | ==Crystal Structure of Phosphorylated C.elegans Thymidylate Synthase in Complex with dUMP== | ||
<StructureSection load='4isw' size='340' side='right' caption='[[4isw]], [[Resolution|resolution]] 3.14Å' scene=''> | <StructureSection load='4isw' size='340' side='right'caption='[[4isw]], [[Resolution|resolution]] 3.14Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4isw]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4isw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ISW FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.14Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4isw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4isw OCA], [https://pdbe.org/4isw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4isw RCSB], [https://www.ebi.ac.uk/pdbsum/4isw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4isw ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9Y052_CAEEL Q9Y052_CAEEL] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 17: | Line 18: | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4isw" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Thymidylate synthase|Thymidylate synthase]] | *[[Thymidylate synthase 3D structures|Thymidylate synthase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Caenorhabditis elegans]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Banaszak | [[Category: Banaszak K]] | ||
[[Category: Dowiercial | [[Category: Dowiercial A]] | ||
[[Category: Jarmula | [[Category: Jarmula A]] | ||
[[Category: Rode | [[Category: Rode W]] | ||
[[Category: Rypniewski | [[Category: Rypniewski W]] | ||
[[Category: Wilk | [[Category: Wilk P]] | ||
Latest revision as of 13:59, 6 November 2024
Crystal Structure of Phosphorylated C.elegans Thymidylate Synthase in Complex with dUMPCrystal Structure of Phosphorylated C.elegans Thymidylate Synthase in Complex with dUMP
Structural highlights
FunctionPublication Abstract from PubMedCrystal structure is presented of the binary complex between potassium phosphoramidate-phosphorylated recombinant C. elegans thymidylate synthase and dUMP. On each monomer a single phosphoserine residue (Ser127) was identified, instead of expected phosphohistidine. As 31P NMR studies of both the phosphorylated protein and of potassium phosphoramidate potential to phosphorylate different amino acids point to histidine as the only possible site of the modification, thermodynamically favored intermolecular phosphotransfer from histidine to serine is suggested. Crystal structure of phosphoramide-phosphorylated thymidylate synthase reveals pSer127, reflecting probably pHis to pSer phosphotransfer.,Wilk P, Jarmula A, Ruman T, Banaszak K, Rypniewski W, Ciesla J, Dowiercial A, Rode W Bioorg Chem. 2013 Nov 21;52C:44-49. doi: 10.1016/j.bioorg.2013.11.003. PMID:24321279[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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