4idm: Difference between revisions

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-{{STRUCTURE_4idm|  PDB=4idm  |  SCENE=  }}
-===Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis===
-{{ABSTRACT_PUBMED_24699642}}
-==About this Structure==
+==Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis==
-[[4idm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IDM OCA].
+<StructureSection load='4idm' size='340' side='right'caption='[[4idm]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4idm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IDM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IDM FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4idm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4idm OCA], [https://pdbe.org/4idm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4idm RCSB], [https://www.ebi.ac.uk/pdbsum/4idm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4idm ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/O50443_MYCTU O50443_MYCTU]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The proline-utilization pathway in Mycobacterium tuberculosis (Mtb) has recently been identified as an important factor in Mtb persistence in vivo, suggesting that this pathway could be a valuable therapeutic target against tuberculosis (TB). In Mtb, two distinct enzymes perform the conversion of proline into glutamate: the first step is the oxidation of proline into Delta(1)-pyrroline-5-carboxylic acid (P5C) by the flavoenzyme proline dehydrogenase (PruB), and the second reaction involves converting the tautomeric form of P5C (glutamate-gamma-semialdehyde) into glutamate using the NAD(+)-dependent Delta(1)-pyrroline-5-carboxylic dehydrogenase (PruA). Here, the three-dimensional structures of Mtb-PruA, determined by X-ray crystallography, in the apo state and in complex with NAD(+) are described at 2.5 and 2.1 A resolution, respectively. The structure reveals a conserved NAD(+)-binding mode, common to other related enzymes. Species-specific conformational differences in the active site, however, linked to changes in the dimer interface, suggest possibilities for selective inhibition of Mtb-PruA despite its reasonably high sequence identity to other PruA enzymes. Using recombinant PruA and PruB, the proline-utilization pathway in Mtb has also been reconstituted in vitro. Functional validation using a novel NMR approach has demonstrated that the PruA and PruB enzymes are together sufficient to convert proline to glutamate, the first such demonstration for monofunctional proline-utilization enzymes.
-==Reference==
+Characterization of the proline-utilization pathway in Mycobacterium tuberculosis through structural and functional studies.,Lagautriere T, Bashiri G, Paterson NG, Berney M, Cook GM, Baker EN Acta Crystallogr D Biol Crystallogr. 2014 Apr 1;70(Pt 4):968-80. doi:, 10.1107/S1399004713034391. Epub 2014 Mar 19. PMID:24699642<ref>PMID:24699642</ref>
-<ref group="xtra">PMID:024699642</ref><references group="xtra"/><references/>
-[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Myctu]]
+</div>
-[[Category: Baker, E N.]]
+<div class="pdbe-citations 4idm" style="background-color:#fffaf0;"></div>
-[[Category: Bashiri, G.]]
-[[Category: Lagautriere, T.]]
+==See Also==
-[[Category: Aldehyde dehydrogenase]]
+*[[Pyrroline-5-carboxylate dehydrogenase|Pyrroline-5-carboxylate dehydrogenase]]
-[[Category: Dehydrogenation]]
+== References ==
-[[Category: Oxidoreductase]]
+<references/>
-[[Category: Pyrroline dehydrogenase]]
+__TOC__
-[[Category: Pyrroline-5-carboxylate dehydrogeanse]]
+</StructureSection>
-[[Category: Pyrroline-5-carboxylic acid]]
+[[Category: Large Structures]]
+[[Category: Mycobacterium tuberculosis H37Rv]]
+[[Category: Baker EN]]
+[[Category: Bashiri G]]
+[[Category: Lagautriere T]]