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==MYCOBACTERIUM TUBERCULOSIS CHORISMATE SYNTHASE after exposure to 266nm UV laser== | |||
<StructureSection load='4baj' size='340' side='right'caption='[[4baj]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4baj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BAJ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4baj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4baj OCA], [https://pdbe.org/4baj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4baj RCSB], [https://www.ebi.ac.uk/pdbsum/4baj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4baj ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/AROC_MYCTU AROC_MYCTU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation- damage induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins. | |||
In-house UV radiation damage induced phasing of selenomethionine-labeled protein structures.,Pereira PJ, Royant A, Panjikar S, de Sanctis D J Struct Biol. 2012 Nov 20. pii: S1047-8477(12)00315-2. doi:, 10.1016/j.jsb.2012.11.003. PMID:23178456<ref>PMID:23178456</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4baj" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Chorismate synthase|Chorismate synthase]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mycobacterium tuberculosis]] | |||
[[Category: Panjikar S]] | |||
[[Category: Pereira PJB]] | |||
[[Category: Royant A]] | |||
[[Category: De Sanctis D]] | |||
Latest revision as of 09:22, 17 October 2024
MYCOBACTERIUM TUBERCULOSIS CHORISMATE SYNTHASE after exposure to 266nm UV laserMYCOBACTERIUM TUBERCULOSIS CHORISMATE SYNTHASE after exposure to 266nm UV laser
Structural highlights
FunctionPublication Abstract from PubMedSelenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation- damage induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins. In-house UV radiation damage induced phasing of selenomethionine-labeled protein structures.,Pereira PJ, Royant A, Panjikar S, de Sanctis D J Struct Biol. 2012 Nov 20. pii: S1047-8477(12)00315-2. doi:, 10.1016/j.jsb.2012.11.003. PMID:23178456[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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