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== | ==1.25 A Structure of Lysozyme Crystallized with (RS)-2-methyl-2,4- pentanediol== | ||
[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK | <StructureSection load='4b4j' size='340' side='right'caption='[[4b4j]], [[Resolution|resolution]] 1.25Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4b4j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B4J FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b4j OCA], [https://pdbe.org/4b4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b4j RCSB], [https://www.ebi.ac.uk/pdbsum/4b4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b4j ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization. | |||
Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol.,Stauber M, Jakoncic J, Berger J, Karp JM, Axelbaum A, Sastow D, Buldyrev SV, Hrnjez BJ, Asherie N Acta Crystallogr D Biol Crystallogr. 2015 Mar 1;71(Pt 3):427-41. doi:, 10.1107/S1399004714025061. Epub 2015 Feb 26. PMID:25760593<ref>PMID:25760593</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4b4j" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[ | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | ||
== References == | |||
== | <references/> | ||
<references | __TOC__ | ||
</StructureSection> | |||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Asherie | [[Category: Asherie N]] | ||
[[Category: Axelbaum | [[Category: Axelbaum A]] | ||
[[Category: Berger | [[Category: Berger J]] | ||
[[Category: Jakoncic | [[Category: Jakoncic J]] | ||
[[Category: Stauber | [[Category: Stauber M]] | ||
Latest revision as of 13:40, 6 November 2024
1.25 A Structure of Lysozyme Crystallized with (RS)-2-methyl-2,4- pentanediol1.25 A Structure of Lysozyme Crystallized with (RS)-2-methyl-2,4- pentanediol
Structural highlights
FunctionLYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedChiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization. Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol.,Stauber M, Jakoncic J, Berger J, Karp JM, Axelbaum A, Sastow D, Buldyrev SV, Hrnjez BJ, Asherie N Acta Crystallogr D Biol Crystallogr. 2015 Mar 1;71(Pt 3):427-41. doi:, 10.1107/S1399004714025061. Epub 2015 Feb 26. PMID:25760593[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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