4ayb: Difference between revisions

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-[[Image:4ayb.jpg|left|200px]]
-{{STRUCTURE_4ayb|  PDB=4ayb  |  SCENE=  }}
+==RNAP at 3.2Ang==
+<StructureSection load='4ayb' size='340' side='right'caption='[[4ayb]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4ayb]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_shibatae Saccharolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AYB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AYB FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.202&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ayb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ayb OCA], [https://pdbe.org/4ayb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ayb RCSB], [https://www.ebi.ac.uk/pdbsum/4ayb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ayb ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RPO1N_SACSH RPO1N_SACSH] DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.[HAMAP-Rule:MF_00863]<ref>PMID:19419240</ref> <ref>PMID:21265742</ref> <ref>PMID:22848102</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Multi-subunit RNA polymerases (RNAPs) in all three domains of life share a common ancestry. The composition of the archaeal RNAP (aRNAP) is not identical between phyla and species, with subunits Rpo8 and Rpo13 found in restricted subsets of archaea. While Rpo8 has an ortholog, Rpb8, in the nuclear eukaryal RNAPs, Rpo13 lacks clear eukaryal orthologs. Here, we report crystal structures of the DNA-bound and free form of the aRNAP from Sulfolobus shibatae. Together with biochemical and biophysical analyses, these data show that Rpo13 C-terminus binds non-specifically to double-stranded DNA. These interactions map on our RNAP-DNA binary complex on the downstream DNA at the far end of the DNA entry channel. Our findings thus support Rpo13 as a RNAP-DNA stabilization factor, a role reminiscent of eukaryotic general transcriptional factors. The data further yield insight into the mechanisms and evolution of RNAP-DNA interaction.
-===RNAP at 3.2Ang===
+Structural and functional analyses of the interaction of archaeal RNA polymerase with DNA.,Wojtas MN, Mogni M, Millet O, Bell SD, Abrescia NG Nucleic Acids Res. 2012 Jul 30. PMID:22848102<ref>PMID:22848102</ref>
-{{ABSTRACT_PUBMED_22848102}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4ayb" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[4ayb]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_shibatae Sulfolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AYB OCA].
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:022848102</ref><ref group="xtra">PMID:021265742</ref><ref group="xtra">PMID:019419240</ref><references group="xtra"/>
+__TOC__
-[[Category: DNA-directed RNA polymerase]]
+</StructureSection>
-[[Category: Sulfolobus shibatae]]
+[[Category: Large Structures]]
-[[Category: Abrescia, N G.A.]]
+[[Category: Saccharolobus shibatae]]
-[[Category: Bell, S D.]]
+[[Category: Abrescia NGA]]
-[[Category: Millet, O.]]
+[[Category: Bell SD]]
-[[Category: Mogni, M.]]
+[[Category: Millet O]]
-[[Category: Wojtas, M N.]]
+[[Category: Mogni M]]
-[[Category: Multi-subunit]]
+[[Category: Wojtas MN]]
-[[Category: Transcription]]
-[[Category: Transferase]]