4ay1: Difference between revisions

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-[[Image:4ay1.jpg|left|200px]]
-{{STRUCTURE_4ay1|  PDB=4ay1  |  SCENE=  }}
+==Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide binding properties==
+<StructureSection load='4ay1' size='340' side='right'caption='[[4ay1]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4ay1]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AY1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AY1 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ay1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ay1 OCA], [https://pdbe.org/4ay1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ay1 RCSB], [https://www.ebi.ac.uk/pdbsum/4ay1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ay1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CH3L2_HUMAN CH3L2_HUMAN] Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.<ref>PMID:22742450</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The chitinase-like proteins YKL-39 (chitinase 3-like-2) and YKL-40 (chitinase 3-like-1) are highly expressed in a number of human cells independent of their origin (mesenchymal, epithelial or haemapoietic). Elevated serum levels of YKL-40 have been associated with a negative outcome in a number of diseases ranging from cancer to inflammation and asthma. YKL-39 expression has been associated with osteoarthritis. However, despite the reported association with disease, the physiological or pathological role of these proteins is still very poorly understood. Although YKL-39 is homologous to the two family 18 chitinases in the human genome, it has been reported to lack any chitinase activity. In the present study, we show that human YKL-39 possesses a chitinase-like fold, but lacks key active-site residues required for catalysis. A glycan screen identified oligomers of N-acetylglucosamine as preferred binding partners. YKL-39 binds chitooligosaccharides and a newly synthesized derivative of the bisdionin chitinase-inhibitor class with micromolar affinity, through a number of conserved tryptophan residues. Strikingly, the chitinase activity of YKL-39 was recovered by reverting two non-conservative substitutions in the active site to those found in the active enzymes, suggesting that YKL-39 is a pseudo-chitinase with retention of chitinase-like ligand-binding properties.
-===Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide binding properties===
+Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide-binding properties.,Schimpl M, Rush CL, Betou M, Eggleston IM, Recklies AD, van Aalten DM Biochem J. 2012 Aug 15;446(1):149-57. PMID:22742450<ref>PMID:22742450</ref>
-{{ABSTRACT_PUBMED_22742450}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4ay1" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[4ay1]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AY1 OCA].
+*[[Chitinase-3-like protein 3D structures|Chitinase-3-like protein 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:022742450</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Aalten, D M.F Van.]]
+[[Category: Large Structures]]
-[[Category: Betou, M.]]
+[[Category: Betou M]]
-[[Category: Eggleston, I M.]]
+[[Category: Eggleston IM]]
-[[Category: Penman, G A.]]
+[[Category: Penman GA]]
-[[Category: Recklies, A D.]]
+[[Category: Recklies AD]]
-[[Category: Rush, C L.]]
+[[Category: Rush CL]]
-[[Category: Schimpl, M.]]
+[[Category: Schimpl M]]
-[[Category: Chilectin]]
+[[Category: Van Aalten DMF]]
-[[Category: Chitooligosaccharide]]
-[[Category: Hydrolase]]
-[[Category: Lectin]]
-[[Category: Pseudochitinase]]