9ins: Difference between revisions

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-[[Image:9ins.png|left|200px]]
-<!--
+==MONOVALENT CATION BINDING IN CUBIC INSULIN CRYSTALS==
-The line below this paragraph, containing "STRUCTURE_9ins", creates the "Structure Box" on the page.
+<StructureSection load='9ins' size='340' side='right'caption='[[9ins]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[9ins]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9INS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9INS FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ins FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ins OCA], [https://pdbe.org/9ins PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ins RCSB], [https://www.ebi.ac.uk/pdbsum/9ins PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ins ProSAT]</span></td></tr>
-{{STRUCTURE_9ins|  PDB=9ins  |  SCENE=  }}
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/in/9ins_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=9ins ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Two localized monovalent cation binding sites have been identified in cubic insulin from 2.8 A-resolution difference electron density maps comparing crystals in which the Na+ ions have been replaced by Tl+. One cation is buried in a closed cavity between insulin dimers and is stabilized by interaction with protein carbonyl dipoles in two juxtaposed alternate positions related by the crystal dyad. The second cation binding site, which also involves ligation with carbonyl dipoles, is competitively occupied by one position of two alternate His B10 side chain conformations. The cation occupancy in both sites depends on the net charge on the protein which was varied by equilibrating crystals in the pH range 7-10. Detailed structures of the cation binding sites were inferred from the refined 2-A resolution map of the sodium-insulin crystal at pH 9. At pH 9, the localized monovalent cations account for less than one of the three to four positive counterion charges necessary to neutralize the negative charge on each protein molecule. The majority of the monovalent counterions are too mobile to show up in the electron density maps calculated using data only at resolution higher than 10 A. Monovalent cations of ionic radius less than 1.5 A are required for crystal stability. Replacing Na+ with Cs+, Mg++, Ca++ or La+++ disrupts the lattice order, but crystals at pH 9 with 0.1 M Li+, K+, NH4+, Rb+ or Tl+ diffract to at least 2.8 A resolution.
-===MONOVALENT CATION BINDING IN CUBIC INSULIN CRYSTALS===
+Monovalent cation binding to cubic insulin crystals.,Gursky O, Li Y, Badger J, Caspar DL Biophys J. 1992 Mar;61(3):604-11. PMID:1504238<ref>PMID:1504238</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_1504238}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 9ins" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 1504238 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_1504238}}
-==About this Structure==
-[[9ins]] is a 2 chain structure of [[Molecular Playground/Insulin]] with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9INS OCA].
 ==See Also==
-*[[Molecular Playground/Insulin|Molecular Playground/Insulin]]
+*[[Insulin 3D Structures|Insulin 3D Structures]]
-*[[PDB identification code|PDB identification code]]
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:001504238</ref><references group="xtra"/>
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Sus scrofa]]
-[[Category: Badger, J.]]
+[[Category: Badger J]]
-[[Category: Dodson, G G.]]
+[[Category: Dodson GG]]
-[[Category: Hormone]]