Protegrin: Difference between revisions

<StructureSection load="1pg1" size="400" color=""  spin="on" Scene=  caption="NMR structure of pig protegrin 1, [[1pg1]]" >

 

 

1PG1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PG1 OCA].  1PG1 is an arginine and cysteine rich protein, which forms a double stranded anti-parallel β-sheet structure.  The single chain forms a membrane-bound dimer, of structure [http://proteopedia.org/wiki/index.php/1zy6 1ZY6].  The structure of 1PG1 is similar to that of some other [http://en.wikipedia.org/wiki/Antimicrobial_peptide antimicrobial peptides] such as [http://en.wikipedia.org/wiki/Defensin defensins]<ref>Interaction of human defensins with Escherichia coli. Mechanism of bactericidal activity., Lehrer RI, Barton A, Daher KA, Harwig SS, Ganz T, Selsted ME., J Clin Invest. 1989 Aug;84(2):553-61. PMID: [http://www.ncbi.nlm.nih.gov/pubmed/2668334 2668334]</ref>.  In one study comparing the susceptibility of [http://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis] to 1PG1 and a similar defensin peptide, 1PG1 was shown to be significantly more effective at inactivating the bacteria<ref>Susceptibility of Chlamydia trachomatis to protegrins and defensins., Yasin B, Harwig SS, Lehrer RI, Wagar EA., Infect Immun. 1996 Mar;64(3):709-13. PMID: [http://www.ncbi.nlm.nih.gov/pubmed/8641770 8641770]</ref>. The antimicrobial action of this protein is believed to be due to its ability to create pores in bacterial membranes causing ion leakage <ref>Membrane channel formation by antimicrobial protegrins., Sokolov Y, Mirzabekov T, Martin DW, Lehrer RI, Kagan BL, Biochim Biophys Acta. 1999 Aug 20;1420(1-2):23-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10446287 10446287]</ref>.  This antimicrobial activity has given rise to the idea of using the peptide as a therapeutic for local or systemic infections<ref>Protegrin-1: a broad-spectrum, rapidly microbicidal peptide with in vivo activity, D A Steinberg, M A Hurst, C A Fujii, A H Kung, J F Ho, F C Cheng, D J Loury, and J C Fiddes, Antimicrob Agents Chemother. 1997 August; 41(8): 1738–1742. PMCID: [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC163996/ PMC163996]</ref>.  The protegrin is synthesized as a ca. 149 amino acid precursor with a cathelin-like domain.

 

</StructureSection>