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== Function ==
== Function ==


'''Endonuclease''' (ENN) cleaves phosphodiester bond within polynucleotide chain.  ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome.  ENN is restriction site–specific.  Various types of ENN differ by their mechanism of action.  ENN is used in genetic engineering to make recombinant DNA.  ENN requires a restriction site and a cleavage pattern.  '''ENN-I''' operates on DNA with separate restriction site and cleavage pattern, while '''ENN-II''' operates on overlapping restriction site and cleavage pattern.  Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN<ref>PMID:12483517</ref>.<br />
'''Endonuclease''' (ENN) cleaves phosphodiester bond within polynucleotide chain.  ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome.  ENN is restriction site–specific.  Various types of ENN differ by their mechanism of action.  ENN is used in genetic engineering to make recombinant DNA.  ENN requires a restriction site and a cleavage pattern.   
*'''ENN-I''' operates on DNA with separate restriction site and cleavage pattern.
*'''ENN-II''' operates on overlapping restriction site and cleavage pattern.   
*'''ENN-III''' is a bifunctional DNA glycosylase that removes oxidized pyrimidines from DNA<ref>PMID:30959406</ref>.
*'''ENN-IV''' cleaves the DNA backbone 5' of AP sites<ref>PMID:10458614</ref>.
*'''ENN-V''' removes deaminated bases from DNA<ref>PMID:23263163</ref>.
*'''ENN-VII''' recognizes mixmatches in DNA recombinational heteroduplexes and performs their incision<ref>PMID:21237725</ref>.
*'''ENN-VIII''' removes radiolysis thymine products from phage DNA and cleaves the DNA at AP sites<ref>PMID:9405426</ref>.
*'''Structure-specific ENN''' specific for DNA secondary structure rather than for its sequence<ref>PMID:28327556</ref>.
*'''Intron-encoded ENN''' or '''homing ENN''' are encoded by genes with mobile, self-splicing introns. They promote the movement of DNA sequences from one chromosome location to another<ref>PMID:9665136</ref>. <br />
The '''Cas ENN''' proteins are part of '''CRISPR/Cas''' prokaryotic immune system which confers protection from foreign genetic elements like viruses.  The '''CRISPR''' ('''C'''lustered '''R'''egularly '''I'''nterspersed '''S'''hort '''P'''alindromic '''R'''epeats) are DNA loci which are found in ca. 40% of the bacteria.  The CRISPR/Cas system is being used lately as gene editing tool<ref>PMID:20056882</ref>.  For more details see<br />
The '''Cas ENN''' proteins are part of '''CRISPR/Cas''' prokaryotic immune system which confers protection from foreign genetic elements like viruses.  The '''CRISPR''' ('''C'''lustered '''R'''egularly '''I'''nterspersed '''S'''hort '''P'''alindromic '''R'''epeats) are DNA loci which are found in ca. 40% of the bacteria.  The CRISPR/Cas system is being used lately as gene editing tool<ref>PMID:20056882</ref>.  For more details see<br />
* [[CRISPR-Cas]]<br />
*[[CRISPR-Cas]]
* [[CRISPR-Cas Part II]]<br />
*CRISPR type I-A (Cascade) - see [[CRISPR subtype I-A]]
* [[Cas9]]<br />
*CRISPR type I-B (Cascade) - see [[CRISPR subtype I-B]]
* [[CRISPR-Cas9]]<br />
*CRISPR type I-C (Cascade) - see [[CRISPR subtype I-C]]
* [[CRISPR type I-E (Cascade)]]<br />
*CRISPR type I-E (Cascade) - see [[CRISPR type I-E (Cascade)|CRISPR subtype I-E]]
* [[CRISPR type V]]<br />
*(CRISPR type I-F (Csy1, Csy2, Csy3) - see [[CRISPR subtype I-F]]
* [[CRISPR subtype I-A]]<br />
*CRISPR type III-A (Csm complex) - see [[CRISPR subtype III-A (Csm complex)]]
* [[CRISPR subtype I-B]]<br />
*CRISPR type II-A - see [[CRISPR-Cas9]]
* [[CRISPR subtype I-C]]<br />
*CRISPR type V (Cpf1, C2c1, C2c3) - see [[CRISPR type V]]
* [[CRISPR subtype I-F]]<br />
*CRISPR type VI (Cas13a (previously known as C2c2), Cas13b, Cas13c, Cas13d) - see [[CRISPR type VI]]
* [[CRISPR subtype III-A (Csm complex)]]<br />
* [[CRISPR subtype III-B (Cmr complex)]]<br />
* [[CRISPR subtype V (Cpf1)]]<br />
* [[CRISPR type VI]]<br />
* [[CRISPR type V (Cpf1)]]<br />
 
'''Intron-encoded ENN''' or '''homing ENN''' are encoded by genes with mobile, self-splicing introns. They promote the movement of DNA sequences from one chromosome location to another<ref>PMID:9665136</ref>. <br /> 
See also<br />
See also<br />
* [[Apurinic-Apyrimidinic Endonuclease-1]]<br />
* [[Apurinic-Apyrimidinic Endonuclease-1]]<br />
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</StructureSection>
</StructureSection>
==3D structures of endonuclease==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
{{#tree:id=OrganizedByTopic|openlevels=0|
*ENN-I
**[[1bix]] - hENN – human<br />
**[[5um9]], [[5kse]], [[5k97]] – hENN (mutant) + DNA <br />
**[[1cfr]] – ENN – ''Citrobacter freundii''<br />
**[[1af5]] - CrENN – ''Chlamydomonas reinhardtii''<br />
**[[1fzr]], [[1m0d]], [[1m0i]], [[3cae]] – T7ENN - Enterobacteria phage T7<br />
**[[2g7e]], [[2g7f]] – VcENN – ''Vibrio cholera''<br />
**[[2vnd]] - VcENN (mutant) <br />
**[[2pu3]] - VsENN – ''Vibrio salmonicida''
*ENN-II
**[[1pvi]], [[1pvu]] - ENN – ''Proteus vulgaris''<br />
**[[2wsh]] - T4ENN (mutant) - Enterobacteria phage T4<br />
**[[3w52]], [[4cwm]], [[4cxo]], [[4cxp]], [[4cxv]] – ENN – ''Arabidopsis thaliana''<br />
*ENN-III
**[[2abk]], [[1ako]] - EcENN  – ''Escherichia coli''<br />
**[[1orn]], [[1orp]], [[1p59]] - GsENN + DNA – ''Geobacillus stearothermophilus''<br />
**[[4unf]] – DrENN-1 – ''Deinococcus radiodurans''  <br />
**[[4uob]] – DrENN-3 <br />
*ENN-IV
**[[1qtw]] - EcENN  <br />
**[[2nqh]] - EcENN (mutant) <br />
**[[1qum]] – EcENN + DNA<br />
**[[2nqj]], [[2nq9]], [[4k1g]] - EcENN (mutant) + DNA<br />
**[[1xp3]] - ENN – ''Bacillus anthracis''<br />
**[[3aal]] - GkENN – ''Geobacillus kaustophilus''<br />
**[[3aam]] - TtENN – ''Thermus thermophilus''
*ENN-V
**[[4nsp]] – hENN catalytic domain<br />
**[[1eni]], [[1enj]], [[1enk]], [[2end]] - T4ENN  <br />
**[[1vas]] - T4ENN + DNA<br />
**[[2w35]], [[2w36]], [[4b20]] - TmENN + DNA – ''Thermotoga maritima''<br />
**[[3hd0]] - TmENN <br />
**[[3ga2]] - BsENN (mutant) – ''Bacillus subtilis''<br />
**[[3goc]] - ENN – ''Streptomyces avermitilis''<br />
**[[2fcc]] – T4ENN + DNA<br />
*ENN-VII
**[[1en7]], [[1e7d]] - T4ENN <br />
**[[1e7l]] - T4ENN (mutant) <br />
**[[2qnc]], [[2qnf]] - T4ENN (mutant) + DNA
*ENN-VIII
**[[1tdh]] – hENN <br />
**[[1bix]] – hENN + DNA<br />
**[[1q39]], [[1q39]] -  EcENN  <br />
**[[1q3b]], [[1q3c]] -  EcENN  (mutant) <br />
**[[1k3x]], [[2ea0]], [[1k3w]], [[1k3x]], [[2ea0]], [[2opf]], [[2oq4]] - EcENN + DNA <br />
**[[2opf]], [[2oq4]], [[6fbu]] - EcENN (mutant) + DNA
*AP ENN
**[[3ngf]] - ENN - ''Brucella melitensis''<br />
**[[2j63]] – ENN – ''Leishmania major''<br />
*Cas ENN
**[[1wj9]], [[4ej3]] – TtCas<br />
**[[4n06]] – AfCas1 - ''Archaeoglobus fulgidus'' <br />
**[[3god]] – PaCas1 – ''Pseudomonas aeruginosa''<br />
**[[3nkd]] – EcCas1<br />
**[[4wj0]] – PhCas1 - ''Pyrococcus horikoshii''<br />
**[[4xtk]] – TmCas1<br />
**[[5fcl]] – Cas1 - ''Pectobacterium atrosepticum''<br />
**[[4zkj]] -  SpCas1 – ''Streptococcus pyogenes''<br />
**[[3oq2]] – Cas2 – ''Desulfovibrio vulgaris''<br />
**[[4mak]] – EcCas2<br />
**[[4p6i]], [[4qdl]] – EcCas1 + EcCas2<br />
**[[6qy3]] – GsCas1 + EcCas2 + Csn2 – Cryo EM<br />
**[[3s4l]] – MjCas3 – ''Methanocaldococcus jannaschii''<br />
**[[4qqw]] – TfCas3 – ''Thermobifida fusca''<br />
**[[4qqx]] – TfCas3 + ATP <br />
**[[4qqy]] – TfCas3 + ADP <br />
**[[4qqz]] – TfCas3 + AMPPNP <br />
**[[4q2c]] - TteCas3 – ''Thermobaclum terrenum''<br />
**[[4q2d]] - TteCas3 + ATP<br />
**[[3i4h]] – Cas4 - ''Pyrobaculum calidifontis''<br /
**[[4qr0]], [[4qr1]] -  SpCas9 (Csn1) – ''Streptococcus pyogenes''<br />
**[[4ilr]], [[3zfv]] – SsCas6 <br />
**[[3i4h]] – PfCas6 - ''Pyrococcus furiosus''<br />
**[[4dzd]] – EcCas6e<br />
**[[4qr0]], [[4qr1]] -  SpCas9 (Csn1) – ''Streptococcus pyogenes''<br />
**[[4qr0]], [[4qr1]], [[4cmp]], [[4cmq]] -  SpCas9 <br />
**[[4ogc]], [[4oge]] – Cas9 II-C – ''Actinomyces naeslundii''<br />
**[[3ung]], [[3ur3]], [[4doz]], [[4w8y]] – PfCas10 CMR2 subunit <br />
**[[4h4k]] – PfCas10 CMR2+CMR3 subunits <br />
**[[3w2v]] – PfCas10 CMR2+CMR3 subunits + AMP<br />
**[[3w2w]] – PfCas10 CMR2+CMR3 subunits + ATP<br />
**[[4gkf]] – PfCas10 CMR5 subunit<br />
**[[4an8]], [[4f3e]] – TtCasA (Cse1)<br />
**[[4h3t]] - CasA (Cse1) – ''Acidimicrobium ferrooxidans''<br />
**[[4h79]] - TfCasB (Cse2)<br />
**[[3wa8]] - CasB (Cse2) – ''Meiothermus ruber''<br />
**[[4h7a]] - TtCasB (Cse2)<br />
**[[4tvx]] – EcCasA (Cse1) + CasE<br />
**[[3qhq]] -  Csn2 – ''Streptococcus agalactiae''<br />
**[[3toc]], [[3v7f]] – SpCsn2<br />
**[[3ps0]] – SsCsa2<br />
*Cas ENN complex with RNA and DNA
**[[5dlj]], [[5dqt]], [[5dqu]], [[5dqz]], [[5ds4]], [[5ds5]], [[5ds6]], [[5vvl]], [[5vvk]], [[5vvj]] – EcCas1 + EcCas2 + DNA<br />
**[[5wfe]] – EcCas1 + EcCas2 + IHF-a + IHF-b + DNA<br />
**[[5xvp]], [[5xvo]], [[5xvn]] – Cas1 + Cas2 + DNA – ''Enterococcus faecalis''<br />
**[[6qxt]], [[6qxf]] – GsCas1 (mutant) + EcCas2 + Csn2 + DNA – Cryo EM<br />
**[[4ill]], [[4ilm]] – SsCas6 + RNA - ''Sulfolobus solfataricus''<br />
**[[5uz9]] – PaCsa5f + Cas7f + Cas8f + anti-crispr protein + RNA - Cryo EM<br />
**[[6b48]], [[6b47]], [[6b46]], [[6b45]] – PaCsa6 + Csy1 + Csy2 + Csy3 + anti-crispr protein + RNA - Cryo EM<br />
**[[6b44]] – PaCsa6 + Csy1 + Csy2 + Csy3 + anti-crispr protein + DNA - Cryo EM<br />
**[[6ne0]] – PaCsa6/Csy4 + Csy1 + Csy2 + Csy3 + DNA + RNA – Cryo EM<br />
**[[3pkm]] – PfCas6 + RNA<br />
**[[2y8w]], [[2y8y]], [[3qrp]], [[3qrq]], [[2qrr]] – TtCas6e (Cse3) + RNA<br />
**[[2xli]], [[2xlj]], [[2xlk]], [[4al7]] – PaCas6f (Csy4) + RNA <br />
**[[4al5]], [[4al6]] - PaCas6f (Csy4) (mutant) + RNA <br />
**[[4zt0]] - SpCas9 + RNA <br />
**[[4zt9]] - SpCas9 (mutant) + RNA <br />
**[[5f9r]] - SpCas9 + RNA + DNA<br />
**[[4oo8]], [[4un3]], [[4un4]], [[4un5]], [[5fw1]], [[5fw2]], [[5fw3]], [[5fq5]], [[5b2r]], [[5b2s]], [[5b2t]], [[5y36]], [[6ia6]] - SpCas9 (Csn1) (mutant) + RNA + DNA<br />
**[[5xbl]] - SpCas9 + associated protein + RNA <br />
**[[6ifo]] - SpCas9 + AcriiA2 + RNA <br />
**[[5vw1]] - SpCas9 + AcriiA4 + RNA <br />
**[[6mcc]], [[5vzl]], [[6mcb]] - SpCas9 + AcriiA4 + RNA – Cryo EM<br />
**[[5vgb]] - NmCas9 + AcriiC1 – Neisseria meningitidis <br />
**[[6jdx]], [[6jdj], [[6j9m]]] - NmCas9 + AcriiC2 <br />
**[[6j9n]] - NmCas9 + AcriiC3 <br />
**[[5x2h]], [[5x2g]] – Cas9 + RNA + DNA – ''Campylobacter jejuni''<br />
**[[5czz]] - SaCas9 (Csn1) + RNA + DNA - ''Staphylococcus aureus''<br />
**[[5axw]] - SaCas9 (Csn1) (mutant) + RNA + DNA <br />
**[[6joo]] - Cas9 + RNA + DNA – Corynebacterium diphtheriae<br />
**[[5b2o]], [[5b2p]], [[5b2q]] - FtCas9 (mutant) + RNA + DNA – ''Francisella tularensis''<br />
**[[6i1l]], [[6i1k]], [[6gtg]], [[6gtf]], [[6gte]], [[6gtd]], [[6gtc]] – FtCas12a (mutant) + RNA + DNA <br />
**[[4u7u]] – EcCasA (Cse1) + CasB + CasC + CasD + CasE + RNA<br />
**[[4qyz]], [[5h9f]], [[5h9e]] – EcCasA (Cse1) + CasB + CasC + CasD + CasE + RNA + DNA<br />
**[[5wqe]] – AaCas C2C1 + RNA – ''Alicyclobacillus acidoterrestris''<br />
**[[5u34]] – AaCas C2C1 (mutant) + RNA <br />
**[[5u30]], [[5u31]], [[5u33]] – AaCas C2C1 + RNA + DNA<br />
**[[5kk5]], [[5b43]] – AcCas Cpf1 + RNA + DNA – ''Acidaminococcus''<br />
**[[5xh7]], [[5xh6]] – AcCas Cpf1 (mutant) + RNA + DNA <br />
**[[5ng6]] – FtCas Cpf1 + RNA  <br />
**[[5nfv]], [[5mga]] – FtCas Cpf1 + RNA + DNA <br />
*EcoKI ENN
**[[2ar0]] – EcENN (mutant)<br />
*Ecoo109I ENN
**[[1wtd]] - EcENN<br />
**[[1wte]] – EcENN + DNA<br />
*EcoRI ENN
**[[1qc9]] - EcENN<br />
**[[1eri]], [[1ckq]], [[1cl8]], [[1qps]] – EcENN + DNA<br />
**[[1qrh]], [[1qri]], [[2oxv]] - EcENN (mutant) + DNA
*EcoRII ENN
**[[1na6]] – EcENN<br />
**[[3hqf]], [[3hqg]] - EnENN + DNA
*EcoRV ENN
**[[1rve]], [[1az3]] – EcENN<br />
**[[1az4]] - EcENN (mutant) <br />
**[[2rve]], [[4rve]], [[1rva]], [[1rvb]], [[1rvc]], [[1az0]], [[1bsu]], [[1bua]], [[1bgb]], [[1rv5]], [[1b94]], [[1b95]], [[1eo3]], [[1eo4]], [[1eon]], [[1eoo]], [[1eop]], [[2b0d]], [[2b0e]], [[5hlk]] - EcENN + DNA<br />
**[[1bss]], [[1b96]], [[1b97]], [[1stx]], [[1suz]], [[1sx5]], [[1sx8]], [[2ge5]] - EcENN (mutant) + DNA<br />
**[[5f8a]] - EcENN + DNA + Lu<br />
*BamHI ENN
**[[1bam]] – BaENN – ''Bacillus amyloliquefaciens''<br />
**[[1bhm]], [[3bam]], [[2bam]], [[1esg]] - BaENN + DNA
*Bbvci ENN
**[[6mag]], [[6m9g]], [[6eg7]] – BbENN subunit 2 – ''Brevibacillus brevis''<br />
**[[6maf]] – BbENN subunits 1+2 <br />
*FokI ENN
**[[1fok]] – PoENN + DNA – ''Planomicrobium okeanokoites''<br />
**[[2fok]] – PoENN
*LAGLIDAD ENN
**[[4z1x]] – fyENN + DNA – fission yeast<br />
**[[5e5o]] – ENN + DNA – ''Sordaria macropora''<br />
*PvuII ENN
**[[3pvi]] - PvENN (mutant) + DNA – ''Proteus vulgaris''<br />
**[[1k0z]], [[1h56]], [[3ksk]] - PvENN<br />
**[[1ni0]] - PvENN (mutant) <br />
**[[2pvi]], [[1eyu]], [[1f0o]] - PvENN + DNA
*tRNA-splicing ENN
**[[2gw6]] – hENN subunit sen15 – NMR<br />
**[[1a79]], [[1a76]], [[1a77]] - MjENN  – ''Methanocaldococcus jannaschii''<br />
**[[2cv8]] - ENN  – ''Sulfolobus tokodaii''<br />
**[[2gjw]] - AfENN + RNA –'' Archaeoglobus fulgidus''<br />
**[[1r0v]], [[1r11]] – AfENN
**[[2ohc]], [[2ohe]] - TaENN – ''Thermoplasma acidophilum''<br />
**[[2zyz]] – ENN – ''Pyrobaculum aerophilum''<br />
**[[3iey]] - ENN + NEQ261 – ''Nanoarchaeum equitans''<br />
**[[3ajv]] - ApENN  (mutant) – ''Aeropyrum pernix''<br />
**[[3p1y]] - ApENN /AfENN<br />
**[[3p1z]] - ApENN  + protein
*VSR ENN
**[[1vsr]] – EcENN<br />
**[[1cw0]] - EcENN + DNA
*BglI ENN
**[[1dmu]], [[1d2i]] – BsENN + DNA <br />
**[[1dfm]] - BsENN (mutant) + DNA
*BglII ENN
**[[1es8]] - BsENN
*MunI ENN
**[[1d02]] - ENN (mutant) + DNA - ''Mycoplasma''
*Sm ENN
**[[1smn]], [[1qae]], [[1ql0]], [[1g8t]] – ENN – ''Serratia marcescens''
*NaeI ENN
**[[1ev7]] - LaENN – ''Lechevalieria aerocolonigenes''<br />
**[[1iaw]] - LaENN + DNA
*NgoMIV ENN
**[[1fiu]], [[4abt]] - ENN + DNA – ''Neisseria gonorrheae''
*NEI ENN
**[[1tdh]] - hENN
*BsoBI ENN
**[[1dc1]] - GsENN + DNA
*Bse634I ENN
**[[1knv]] – GsENN<br />
**[[3v1z]] – GsENN + DNA<br />
**[[3v20]], [[3v21]] - GsENN (mutant) + DNA
*HincII ENN
**[[1kc6]], [[1tw8]], [[1xhu]], [[1xhv]], [[1tx3]], [[2gie]] - HiENN + DNA – ''Haemophilus influenza''<br />
**[[2gig]], [[2gih]], [[2gii]], [[2gij]], [[3e3y]], [[3e40]], [[3e41]], [[3e42]], [[3e43]], [[3e44]], [[3e45]], [[3ebc]] - HiENN (mutant) + DNA<br />
**[[2aud]] – HiENN
*HinDIII ENN
**[[2e52]], [[3a4k]] - HiENN + DNA
*HinP1I ENN
**[[1ynm]] – HiENN<br />
**[[2fkc]], [[2fkh]], [[2fl3]], [[2flc]] - HiENN + DNA
*Flap ENN
**[[5zod]] - hENN <br />
**[[3q8k]], [[3q8l]], [[3q8m]] - hENN + DNA<br />
**[[5zog]], [[5zof]], [[5zoe]] - hENN (mutant) + DNA<br />
**[[5fv7]] - hENN + inhibitor<br />
**[[1ul1]] - hENN + PCNA <br />
**[[1mc8]] - PhENN (mutant) <br />
**[[3ory]]– ENN – ''Desulfurococcus amylolyticus''<br />
**[[1rxv]], [[1rxw]] - AfENN + DNA<br />
**[[2izo]] - SsENN + PCNA – ''Sulfolobus solfataricus''<br />
**[[1a76]], [[1a77]] - MjENN  <br />
**[[1b43]] – PfENN – ''Pyrococcus furiosus''<br />
**[[1rxz]] - AfENN + PCNA <br />
**[[4wa8]] – ENN – ''Methanopyrus kandleri''<br />
*DNA mismatch ENN
**[[1h7u]] – hENN<br />
**[[6mfq]] – hENN Pms2<br />
**[[5u5r]] – hENN + importin<br />
**[[1odg]] - EcENN + DNA<br />
*Vvn ENN
**[[1ouo]] – VvENN – ''Vibrio vulnificus''<br />
**[[1oup]], [[2ivk]] - VvENN + DNA
*Structure-specific ENN
**[[4zou]] – hENN SLX4 subunit BTB domain<br />
**[[4uyi]] – hENN SLX4 subunit BTB domain (mutant)<br />
**[[4xm5]] – CgENN SLX1 subunit – ''Candida glabrata''<br />
**[[4xlg]] – CgENN SLX1 (mutant)+SLX4 subunits<br />
**[[4zdt]] – fyENN SLX1+SLX4 subunits<br />
*BfiI ENN
**[[2c1l]] – ENN – ''Bacillus firmus''
*SfiI ENN
**[[2ezv]], [[2f03]] – ENN + DNA – synthetic
*Ecl18kI ENN
**[[2fqz]], [[2gb7]] – ENN + DNA – ''Enterobacter cloacae''
*SdaI ENN
**[[2ixs]] – ENN – ''Streptomyces diastaticus''
*PabI ENN
**[[2dvy]] – ENN – ''Pyrococcus abyssi''
*PspGI ENN
**[[3bm3]] – ENN – ''Pyrococcus''
*HNH ENN
**[[4h6d]] – ENN – ''Geobacter metallireducens''<br />
**[[5h0o]], [[5h0m]] – ENN – Geobacillus virus E2<br />
**[[5cxr]] – ENN + pyrazole derivative – Influenza virus <br />
**[[6j9l]]] - NmENN + AcriiC2 <br />
*UV damage ENN
**[[2j6v]], [[3bzg]], [[3c0s]] – TtENN <br />
**[[3bzj]], [[3c0l]], [[3c0q]] - TtENN (mutant)<br />
**[[3tc3]] - SaENN <br />
**[[4gle]] - SaENN + DNA<br />
*NotI ENN
**[[3bvq]] - NoENN – ''Nocardia otitidiscaviarum''<br />
**[[3c25]] - NoENN + DNA
*BpujI ENN
**[[2vla]], [[3s1s]] – BpENN – ''Bacillus pumilus''<br />
**[[5hnf]], [[5hnh]], [[5hlt]] – BpENN + DNA <br />
*Sau3AI ENN
**[[2reu]] – SaENN C terminal – ''Staphylococcus aureus''<br />
**[[4pxg]] – SaENN (mutant)  <br />
*HpyI ENN
**[[3fc3]], [[3gox]] – HpENN99 + DNA – ''Helicobacter pylori''<br />
**[[3or3]] – HpENN88 + DNA
*PacI ENN
**[[3ldy]], [[3m7k]] - PaENN + DNA – ''Psdeudomonas alcaligenes''
*Crossover junction ENN
**[[2ziw]], [[2zix]], [[2ziu]] – hENN EME1 + Mus81<br />
**[[2ziv]] – hENN EME1 (mutant) + Mus81<br />
**[[4p0p]], [[4p0q]], [[4p0r]], [[4p0s]] – hENN EME1 + Mus81 + DNA<br />
**[[2kpf]] - mENN – mouse<br />
*OkrAI ENN
**[[3odh]] - ENN + DNA – ''Oceanobacter kriegii''
*BspD6I ENN
**[[2ewf]] - ENN – ''Bacillus''
*ThaI ENN
**[[3ndh]] - TaENN + DNA
*Xpf ENN
**[[1z00]], [[2a1j]] - hENN C terminal + DNA excision repair protein<br />
**[[2aq0]] – hENN residues 823-905 – NMR<br />
**[[2kn7]] – hENN residues 823-905 + DNA – NMR<br />
**[[2bgw]] - ApENN + DNA <br />
**[[2bhn]] - ApENN <br />
*Mazf3 ENN
**[[5uct]], [[5cca]] - MtENN – ''Mycobacterium tuberculosis''<br />
*Vapc ENN
**[[5wzf]], [[5wz4]] – MtENN-20 <br />
**[[5ed0]], [[5ecy]] – SfENN – ''Shigella flexneri''<br />
**[[5ecy]], [[5ecw]], [[5ecd]] – SfENN (mutant)<br />
*Muts2 ENN
**[[4och]], [[4od6]] - DrENN SMR domain<br />
*Endoms ENN
**[[5gkj]] – TkENN (mutant) – ''Thermococcus kodakarensis''<br />
**[[5gke]], [[5gkf]], [[5gkg]], [[5gkh]], [[5gki]] – TkENN (mutant) + DNA<br />
*ENN G
**[[5gkc]], [[4qn0]] – CeENN (mutant)  – ''Caenorhabdfitis elegans''<br />
**[[5gkp]] – CeENN (mutant) + DNA  <br />
*ENN S
**[[5ybb]] – ENN + R-M system + DNA – ''Caldanaerobacter subterraneus''  <br />
*ENN V
**[[5aoy]] – mENN (mutant)  <br />
**[[4xpu]] – EcENN  <br />
*Apurinic-apyrimidinic ENN
**[[5cfg]], [[6mk3]], [[6mkk]], [[6mkm]], [[6mko]] – hENN <br />
**[[5dff]], [[5dfh]], [[5dfi]], [[5dfj]], [[5dg0]], [[5wn5]], [[5wn4]], [[5wn3]], [[5wn2]], [[5wn1]], [[5wn0]], [[6bos]]  – hENN + DNA<br />
**[[6bot]], [[6boq]], [[6bor]], [[6bou]], [[6bov]], [[6bow]] – hENN (mutant) + DNA<br />
*'''Intron-encoded endonuclease'''
*I-PpoI ENN
**[[1a74]], [[1ipp]], [[1a73]], [[1cz0]] - PpENN + DNA – ''Physarum polycephalum''<br />
**[[1cyq]], [[1evw]], [[2o6m]] - PpENN (mutant) + DNA<br />
**[[1evx]] - PpENN
*I-CreI ENN
**[[1bp7]], [[1g9y]], [[1g9z]], [[1n3e]], [[1n3f]], [[2vbj]], [[2vbl]], [[2vbn]], [[2vbo]], [[4aqu]], [[4aqx]] - CrENN + DNA <br />
**[[1u0c]], [[1u0d]], [[1t9i]], [[1t9j]], [[2i3p]], [[2i3q]], [[4aab]], [[4aad]], [[4aae]], [[4aaf]], [[4aag]], [[6fb9]], [[6fb8]], [[6fb7]], [[6fb6]], [[6fb5]], [[6fb2]], [[6fb1]], [[6fb0]] - CrENN (mutant) + DNA<br />
**[[2o7m]] - CrENN
*I-CeuI ENN
**[[2ex5]] - ENN (mutant) + DNA – ''Chlamydomonas eugametos''
*I-DmoI ENN
**[[1b24]], [[2vs7]], [[2vs8]], [[4un7]], [[4un8]], [[4un9]], [[4una]], [[4unb]], [[4unc]], [[4ut0]], [[4d6n]], [[4d6o]], [[5ak9]], [[5a0w]], [[5o6g]], [[5o6i]] - DmENN + DNA – ''Desulfurococcus mobilis''<br />
**[[5akf]], [[5akm]], [[5akn]] - DmENN (mutant) + DNA <br />
**[[1mow]] – DmENN/CrENN + DNA
*I-AniI ENN
**[[1p8k]], [[2qoj]], [[3uvf]] - EnENN + DNA – ''Emericella nidulans''<br />
**[[3eh8]] - EnENN (mutant) + DNA
*I-HmuI ENN
**[[1u3e]] - ENN + DNA – Bacillus phage spo1
*I-TevI ENN
**[[1i3j]], [[1t2t]] - T4ENN + DNA<br />
**[[1ln0]], [[1mk0]] – T4ENN (mutant)
*I-SceI ENN
**[[1dfa]], [[1ef0]] – yENN – yeast<br />
**[[1gpp]] – yENN (mutant) <br />
**[[1lws]], [[1lwt]], [[1r7m]], [[3c0w]], [[3c0x]], [[3ool]], [[5a0m]] - yENN + DNA<br />
**[[3oor]] - yENN (mutant) + DNA
*I-PkoII ENN
**[[2cw7]], [[2cw8]] – TkENN
*I-MspI ENN
**[[1sa3]], [[1yfi]], [[2fld]] – ENN + DNA – ''Moraxella''
*I-PfuI ENN
**[[1dq3]] – PfENN
*I-Tsp061I ENN
**[[2dch]] – ENN – ''Thermoproteus''
*I-Vdi141I ENN
**[[3e54]] – ENN – ''Vulcanisaeta distribute''
*I-MtuI ENN
**[[3ifj]], [[3igd]] - MtENN (mutant) <br />
**[[2l8l]] - MtENN
*I-MsoI ENN
**[[1m5x]], [[3fd2]] – MoENN + DNA – ''Monomastix''<br />
**[[3ko2]] – MoENN (mutant) + DNA
*I-GzeMII ENN
**[[4efj]] – ENN + DNA – ''Gibberella zeae''
*I-tRNA ENN
**[[4fz2]] – ENN – ''Candidatus micrarchaeum''<br />
*I-Cvui ENN
**[[5a78]], [[5a72]], [[5a74]], [[5a77]], [[5a78]] – ENN + DNA – ''Chlorella vulgaris''<br />


}}
== References ==
== References ==
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<references/>
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[[Category:Topic Page]]
[[Category:Topic Page]]
[[Category:Endonuclease]]

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman
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