4exn: Difference between revisions

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-[[Image:4exn.jpg|left|200px]]
-<!--
+==Crystal structure of mouse Interleukin-34==
-The line below this paragraph, containing "STRUCTURE_4exn", creates the "Structure Box" on the page.
+<StructureSection load='4exn' size='340' side='right'caption='[[4exn]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[4exn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EXN FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-{{STRUCTURE_4exn|  PDB=4exn  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4exn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4exn OCA], [https://pdbe.org/4exn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4exn RCSB], [https://www.ebi.ac.uk/pdbsum/4exn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4exn ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IL34_MOUSE IL34_MOUSE] Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1 (By similarity).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Interleukin-34 (IL-34) and colony stimulating factor-1 (CSF-1) both signal through the CSF-1R receptor tyrosine kinase, but they have no sequence homology, and their functions and signaling activities are not identical. We report the crystal structures of mouse IL-34 alone and in complex with the N-terminal three immunoglobulin-like domains (D1-D3) of mouse CSF-1R. IL-34 is structurally related to other helical hematopoietic cytokines, but contains two additional helices integrally associated with the four shared helices. The non-covalently linked IL-34 homodimer recruits two copies of CSF-1R on the sides of the helical bundles, with an overall shape similar to the CSF-1:CSF-1R complex, but the flexible linker between CSF-1R D2 and D3 allows these domains to clamp IL-34 and CSF-1 at different angles. Functional dissection of the IL-34:CSF-1R interface indicates that the hydrophobic interactions, rather than the salt bridge network, dominate the biological activity of IL-34. To degenerately recognize two ligands with completely different surfaces, CSF-1R apparently takes advantage of different subsets of a chemically inert surface that can be tuned to fit different ligand shapes. Differentiated signaling between IL-34 and CSF-1 is likely achieved by the relative thermodynamic independence of IL-34 vs. negative cooperativity of CSF-1 at the receptor-recognition sites, in combination with the difference in hydrophobicity which dictates a more stable IL-34:CSF-1R complex compared to the CSF-1:CSF-1R complex.
-===Crystal structure of mouse Interleukin-34===
+The mechanism of shared but distinct CSF-1R signaling by the non-homologous cytokines IL-34 and CSF-1.,Liu H, Leo C, Chen X, Wong BR, Williams LT, Lin H, He X Biochim Biophys Acta. 2012 May 8. PMID:22579672<ref>PMID:22579672</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4exn" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_22579672}}, adds the Publication Abstract to the page
+*[[Interleukin 3D structures|Interleukin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 22579672 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_22579672}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-[[4exn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXN OCA].
-==Reference==
-<ref group="xtra">PMID:022579672</ref><references group="xtra"/>
 [[Category: Mus musculus]]
-[[Category: Chen, X.]]
+[[Category: Chen X]]
-[[Category: He, X.]]
+[[Category: He X]]
-[[Category: Leo, C.]]
+[[Category: Leo C]]
-[[Category: Lin, H.]]
+[[Category: Lin H]]
-[[Category: Liu, H.]]
+[[Category: Liu H]]
-[[Category: Williams, L T.]]
+[[Category: Williams LT]]
-[[Category: Wong, B R.]]
+[[Category: Wong BR]]
-[[Category: C-fm]]
-[[Category: Csf-1r]]
-[[Category: Cytokine]]
-[[Category: Extended 4-helix bundle]]