4eue: Difference between revisions
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==Crystal structure of Clostridium acetobutulicum trans-2-enoyl-CoA reductase in complex with NADH== | |||
<StructureSection load='4eue' size='340' side='right'caption='[[4eue]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4eue]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_acetobutylicum_ATCC_824 Clostridium acetobutylicum ATCC 824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EUE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eue OCA], [https://pdbe.org/4eue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eue RCSB], [https://www.ebi.ac.uk/pdbsum/4eue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eue ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/FABV_CLOAB FABV_CLOAB] Involved in the fatty acid synthesis (FAS II). Catalyzes the reduction of the carbon-carbon double bond of crotonyl-CoA to yield butyryl-CoA.<ref>PMID:23050861</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Trans-2-enoyl-CoA reductases (TERs, EC 1.3.1.44), which specifically catalyze the reduction of crotonyl-CoA to butyryl-CoA using NADH as cofactor, have recently been applied in the design of robust synthetic pathways to produce 1-butanol as a biofuel. We report here the characterization of a TER homologue in Clostridium acetobutylicum (CaTER), the structures of CaTER in apo form and in complexes with NADH and NAD+, and the structure of Treponema denticola TER (TdTER) in complex with NAD+. Structural and sequence comparisons show that CaTER and TdTER share about 45% overall sequence identity and high structural similarities with the FabV class enoyl-acyl carrier protein reductases in the bacterial fatty acid synthesis pathway, suggesting that both types of enzymes belong to the same family. CaTER and TdTER function as monomers and consist of a cofactor-binding domain and a substrate-binding domain with the catalytic active site located at the interface of the two domains. Structural analyses of CaTER together with mutagenesis and biochemical data indicate that the conserved Glu75 determines the cofactor specificity, and the conserved Tyr225, Tyr235, and Lys244 play critical roles in catalysis. Upon cofactor binding, the substrate-binding loop changes from an open conformation to a closed conformation, narrowing a hydrophobic channel to the catalytic site. A modeling study shows that the hydrophobic channel is optimal in both width and length for the binding of crotonyl-CoA. These results provide molecular bases for the high substrate specificity and the catalytic mechanism of TERs. | |||
Structures of trans-2-enoyl-CoA reductases from Clostridium acetobutulicum and Treponema denticola: insights into the substrate specificity and the catalytic mechanism.,Hu K, Zhao M, Zhang T, Zha M, Zhong C, Jiang Y, Ding J Biochem J. 2012 Oct 11. PMID:23050861<ref>PMID:23050861</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4eue" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Clostridium acetobutylicum ATCC 824]] | |||
[[Category: Large Structures]] | |||
[[Category: Ding J]] | |||
[[Category: Hu K]] | |||
[[Category: Yang S]] | |||
[[Category: Zhang T]] | |||
[[Category: Zhao M]] | |||
Latest revision as of 12:58, 30 October 2024
Crystal structure of Clostridium acetobutulicum trans-2-enoyl-CoA reductase in complex with NADHCrystal structure of Clostridium acetobutulicum trans-2-enoyl-CoA reductase in complex with NADH
Structural highlights
FunctionFABV_CLOAB Involved in the fatty acid synthesis (FAS II). Catalyzes the reduction of the carbon-carbon double bond of crotonyl-CoA to yield butyryl-CoA.[1] Publication Abstract from PubMedTrans-2-enoyl-CoA reductases (TERs, EC 1.3.1.44), which specifically catalyze the reduction of crotonyl-CoA to butyryl-CoA using NADH as cofactor, have recently been applied in the design of robust synthetic pathways to produce 1-butanol as a biofuel. We report here the characterization of a TER homologue in Clostridium acetobutylicum (CaTER), the structures of CaTER in apo form and in complexes with NADH and NAD+, and the structure of Treponema denticola TER (TdTER) in complex with NAD+. Structural and sequence comparisons show that CaTER and TdTER share about 45% overall sequence identity and high structural similarities with the FabV class enoyl-acyl carrier protein reductases in the bacterial fatty acid synthesis pathway, suggesting that both types of enzymes belong to the same family. CaTER and TdTER function as monomers and consist of a cofactor-binding domain and a substrate-binding domain with the catalytic active site located at the interface of the two domains. Structural analyses of CaTER together with mutagenesis and biochemical data indicate that the conserved Glu75 determines the cofactor specificity, and the conserved Tyr225, Tyr235, and Lys244 play critical roles in catalysis. Upon cofactor binding, the substrate-binding loop changes from an open conformation to a closed conformation, narrowing a hydrophobic channel to the catalytic site. A modeling study shows that the hydrophobic channel is optimal in both width and length for the binding of crotonyl-CoA. These results provide molecular bases for the high substrate specificity and the catalytic mechanism of TERs. Structures of trans-2-enoyl-CoA reductases from Clostridium acetobutulicum and Treponema denticola: insights into the substrate specificity and the catalytic mechanism.,Hu K, Zhao M, Zhang T, Zha M, Zhong C, Jiang Y, Ding J Biochem J. 2012 Oct 11. PMID:23050861[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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