4ede: Difference between revisions

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-[[Image:4ede.jpg|left|200px]]
-{{STRUCTURE_4ede|  PDB=4ede  |  SCENE=  }}
+==Crystal Structure of the Q108K:K40L:T51V:T53C:Y19W:R58W:T29L:A33W Mutant of Cellular Retinol Binding Protein Type II in Complex with All-trans-Retinal at 1.4 Angstrom Resolution==
+<StructureSection load='4ede' size='340' side='right'caption='[[4ede]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4ede]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EDE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EDE FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ede FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ede OCA], [https://pdbe.org/4ede PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ede RCSB], [https://www.ebi.ac.uk/pdbsum/4ede PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ede ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RET2_HUMAN RET2_HUMAN] Intracellular transport of retinol.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Protein-chromophore interactions are a central component of a wide variety of critical biological processes such as color vision and photosynthesis. To understand the fundamental elements that contribute to spectral tuning of a chromophore inside the protein cavity, we redesigned human cellular retinol binding protein II (hCRBPII) to fully encapsulate all-trans-retinal and form a covalent bond as a protonated Schiff base. This system, using rational mutagenesis designed to alter the electrostatic environment within the binding pocket of the host protein, enabled regulation of the absorption maximum of the pigment in the range of 425 to 644 nanometers. With only nine point mutations, the hCRBPII mutants induced a systematic shift in the absorption profile of all-trans-retinal of more than 200 nanometers across the visible spectrum.
-===Crystal Structure of the Q108K:K40L:T51V:T53C:Y19W:R58W:T29L:A33W Mutant of Cellular Retinol Binding Protein Type II in Complex with All-trans-Retinal at 1.4 Angstrom Resolution===
+Tuning the electronic absorption of protein-embedded all-trans-retinal.,Wang W, Nossoni Z, Berbasova T, Watson CT, Yapici I, Lee KS, Vasileiou C, Geiger JH, Borhan B Science. 2012 Dec 7;338(6112):1340-3. doi: 10.1126/science.1226135. PMID:23224553<ref>PMID:23224553</ref>
-{{ABSTRACT_PUBMED_23224553}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4ede" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[4ede]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EDE OCA].
+*[[Retinol-binding protein 3D structures|Retinol-binding protein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Geiger, J H.]]
+[[Category: Large Structures]]
-[[Category: Nossoni, Z.]]
+[[Category: Geiger JH]]
-[[Category: Beta barrel]]
+[[Category: Nossoni Z]]
-[[Category: Retinal complex]]
-[[Category: Transport protein]]