4aoe: Difference between revisions
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==Biomphalaria glabrata Acetylcholine-binding protein type 2 (BgAChBP2)== | |||
<SX load='4aoe' size='340' side='right' viewer='molstar' caption='[[4aoe]], [[Resolution|resolution]] 6.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4aoe]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Biomphalaria_glabrata Biomphalaria glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AOE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aoe OCA], [https://pdbe.org/4aoe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aoe RCSB], [https://www.ebi.ac.uk/pdbsum/4aoe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aoe ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/J7JGR6_BIOGL J7JGR6_BIOGL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nicotinic acetylcholine receptors (nAChR) play important neurophysiological roles and are of considerable medical relevance. They have been studied extensively, greatly facilitated by the gastropod acetylcholine-binding proteins (AChBP) which represent soluble structural and functional homologues of the ligand-binding domain of nAChR. All these proteins are ring-like pentamers. Here we report that AChBP exists in the hemolymph of the planorbid snail Biomphalaria glabrata (vector of the schistosomiasis parasite) as a regular pentagonal dodecahedron, 22 nm in diameter (12 pentamers, 60 active sites). We sequenced and recombinantly expressed two approximately 25 kDa polypeptides (BgAChBP1 and BgAChBP2) with a specific active site, N-glycan site and disulfide bridge variation. We also provide the exon/intron structures. Recombinant BgAChBP1 formed pentamers and dodecahedra, recombinant BgAChBP2 formed pentamers and probably disulfide-bridged di-pentamers, but not dodecahedra. Three-dimensional electron cryo-microscopy (3D-EM) yielded a 3D reconstruction of the dodecahedron with a resolution of 6 A. Homology models of the pentamers docked to the 6 A structure revealed opportunities for chemical bonding at the inter-pentamer interfaces. Definition of the ligand-binding pocket and the gating C-loop in the 6 A structure suggests that 3D-EM might lead to the identification of functional states in the BgAChBP dodecahedron. | |||
Acetylcholine-Binding Protein in the Hemolymph of the Planorbid Snail Biomphalaria glabrata Is a Pentagonal Dodecahedron (60 Subunits).,Saur M, Moeller V, Kapetanopoulos K, Braukmann S, Gebauer W, Tenzer S, Markl J PLoS One. 2012;7(8):e43685. Epub 2012 Aug 20. PMID:22916297<ref>PMID:22916297</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4aoe" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
[[ | *[[Acetylcholine binding protein 3D structures|Acetylcholine binding protein 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Biomphalaria glabrata]] | [[Category: Biomphalaria glabrata]] | ||
[[Category: Braukmann | [[Category: Large Structures]] | ||
[[Category: Gebauer | [[Category: Braukmann S]] | ||
[[Category: Kapetanopoulos | [[Category: Gebauer W]] | ||
[[Category: Markl | [[Category: Kapetanopoulos K]] | ||
[[Category: Moeller | [[Category: Markl J]] | ||
[[Category: Saur | [[Category: Moeller V]] | ||
[[Category: Tenzer | [[Category: Saur M]] | ||
[[Category: Tenzer S]] | |||