4dcf: Difference between revisions
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<StructureSection load='4dcf' size='340' side='right'caption='[[4dcf]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='4dcf' size='340' side='right'caption='[[4dcf]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4dcf]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4dcf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_brazili Bothrops brazili]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DCF FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dcf OCA], [https://pdbe.org/4dcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dcf RCSB], [https://www.ebi.ac.uk/pdbsum/4dcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dcf ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/PA2H1_BOTBZ PA2H1_BOTBZ] Snake venom phospholipase A2 homolog that lacks enzymatic activity. Is myotoxic and displays edema-inducing activities in mouse paw (PubMed:18602430). Also displays cytotoxic activity against some cell lines, and antimicrobial activities against E.coli, C.albicans and Leishmania (PubMed:18602430). A model of myotoxic mechanism has been proposed: an apo Lys49-PLA2 is activated by the entrance of a hydrophobic molecule (e.g. fatty acid) at the hydrophobic channel of the protein leading to a reorientation of a monomer (PubMed:24145104). This reorientation causes a transition between 'inactive' to 'active' states, causing alignment of C-terminal and membrane-docking sites (MDoS) side-by-side and putting the membrane-disruption sites (MDiS) in the same plane, exposed to solvent and in a symmetric position for both monomers (PubMed:24145104). The MDoS region stabilizes the toxin on membrane by the interaction of charged residues with phospholipid head groups (PubMed:24145104). Subsequently, the MDiS region destabilizes the membrane with penetration of hydrophobic residues (PubMed:24145104). This insertion causes a disorganization of the membrane, allowing an uncontrolled influx of ions (i.e. calcium and sodium), and eventually triggering irreversible intracellular alterations and cell death (PubMed:24145104).<ref>PMID:18602430</ref> <ref>PMID:24145104</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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[[Category: Bothrops brazili]] | [[Category: Bothrops brazili]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Arni | [[Category: Arni RK]] | ||
[[Category: Betzel | [[Category: Betzel C]] | ||
[[Category: Murakami | [[Category: Murakami MT]] | ||
[[Category: Souza | [[Category: Souza TACB]] | ||
[[Category: Ullah | [[Category: Ullah A]] | ||