3upa: Difference between revisions
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==A general strategy for the generation of human antibody variable domains with increased aggregation resistance== | ==A general strategy for the generation of human antibody variable domains with increased aggregation resistance== | ||
<StructureSection load='3upa' size='340' side='right' caption='[[3upa]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='3upa' size='340' side='right'caption='[[3upa]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3upa]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3upa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UPA FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3upa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3upa OCA], [https://pdbe.org/3upa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3upa RCSB], [https://www.ebi.ac.uk/pdbsum/3upa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3upa ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/KV139_HUMAN KV139_HUMAN] V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).<ref>PMID:17576170</ref> <ref>PMID:20176268</ref> <ref>PMID:22158414</ref> <ref>PMID:24600447</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Christ | [[Category: Large Structures]] | ||
[[Category: Dudgeon | [[Category: Christ D]] | ||
[[Category: Kokmeijer | [[Category: Dudgeon K]] | ||
[[Category: Langley | [[Category: Kokmeijer I]] | ||
[[Category: Rouet | [[Category: Langley DB]] | ||
[[Category: Rouet R]] | |||