3u30: Difference between revisions
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==Crystal structure of a linear-specific Ubiquitin fab bound to linear ubiquitin== | ==Crystal structure of a linear-specific Ubiquitin fab bound to linear ubiquitin== | ||
<StructureSection load='3u30' size='340' side='right' caption='[[3u30]], [[Resolution|resolution]] 2.43Å' scene=''> | <StructureSection load='3u30' size='340' side='right'caption='[[3u30]], [[Resolution|resolution]] 2.43Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3u30]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3u30]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U30 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U30 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.428Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u30 OCA], [https://pdbe.org/3u30 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u30 RCSB], [https://www.ebi.ac.uk/pdbsum/3u30 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u30 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/UBC_HUMAN UBC_HUMAN] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.<ref>PMID:16543144</ref> <ref>PMID:19754430</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3u30" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[ | *[[3D structures of ubiquitin|3D structures of ubiquitin]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Dixit | [[Category: Large Structures]] | ||
[[Category: Dong | [[Category: Dixit VM]] | ||
[[Category: Gao | [[Category: Dong KC]] | ||
[[Category: Hannoush | [[Category: Gao X]] | ||
[[Category: Hymowitz | [[Category: Hannoush RN]] | ||
[[Category: Kelley | [[Category: Hymowitz SG]] | ||
[[Category: Kirkpatrick | [[Category: Kelley RF]] | ||
[[Category: Matsumoto | [[Category: Kirkpatrick DS]] | ||
[[Category: Phu | [[Category: Matsumoto ML]] | ||
[[Category: Yu | [[Category: Phu L]] | ||
[[Category: Yu C]] | |||