Exonuclease: Difference between revisions
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'''Exonucleases''' (ExN) cleave nucleotides at the end of a polynucleotide chain<ref>PMID:14770007</ref>. In ''E. coli'' there are ExN I to VIII. ExN were found in human and yeast as well. | '''Exonucleases''' (ExN) cleave nucleotides at the end of a polynucleotide chain<ref>PMID:14770007</ref>. In ''E. coli'' there are ExN I to VIII. ExN were found in human and yeast as well. | ||
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* '''ExN-I''' is a 5'-3'ExN cleaves double-stranded DNA<ref>PMID:27494243</ref>.<br /> | * '''ExN-I''' is a 5'-3' ExN cleaves double-stranded DNA<ref>PMID:27494243</ref>.<br /> | ||
* '''ExN-II''' is associated with DNA polymerase.<br /> | * '''ExN-II''' is associated with DNA polymerase.<br /> | ||
* '''ExN-III''' cleaves double-stranded DNA.<br /> | * '''ExN-III''' cleaves double-stranded DNA.<br /> | ||
* '''ExN-IV''' cleavage results in nucleoside monophosphate.<br /> | * '''ExN-IV''' cleavage results in nucleoside monophosphate.<br /> | ||
* '''ExN-V''' cleaves linear double-stranded DNA.<br /> | * '''ExN-V''' cleaves linear double-stranded DNA.<br /> | ||
* '''ExN-VIII''' requires a free 5’ OH group for reacting.<br /> | * '''ExN-VIII''' degrades linear duplex DNA<ref>PMID:6309804</ref> and requires a free 5’ OH group for reacting.<br /> | ||
* '''ExN-X''' can degrade both single-stranded DNA and duplex DNA with 3'-5' polarity<ref>PMID:10514496</ref> | * '''ExN-X''' can degrade both single-stranded DNA and duplex DNA with 3'-5' polarity<ref>PMID:10514496</ref> | ||
* '''Trex ExN''' degrades single-stranded DNA generated from processing of aberrant replication intermediates<ref>PMID:18045533</ref> | * '''Trex ExN''' degrades single-stranded DNA generated from processing of aberrant replication intermediates<ref>PMID:18045533</ref> | ||