3spe: Difference between revisions
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The | ==Crystal structure of the tail sheath protein protease resistant fragment from bacteriophage phiKZ== | ||
<StructureSection load='3spe' size='340' side='right'caption='[[3spe]], [[Resolution|resolution]] 2.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3spe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phiKZ Pseudomonas virus phiKZ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SPE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SPE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3996Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3spe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3spe OCA], [https://pdbe.org/3spe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3spe RCSB], [https://www.ebi.ac.uk/pdbsum/3spe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3spe ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8SDD3_BPDPK Q8SDD3_BPDPK] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacteriophage phiKZ is a giant phage that infects Pseudomonas aeruginosa, a human pathogen. The phiKZ virion consists of a 1450 A diameter icosahedral head and a 2000 A-long contractile tail. The structure of the whole virus was previously reported, showing that its tail organization in the extended state is similar to the well-studied Myovirus bacteriophage T4 tail. The crystal structure of a tail sheath protein fragment of phiKZ was determined to 2.4 A resolution. Furthermore, crystal structures of two prophage tail sheath proteins were determined to 1.9 and 3.3 A resolution. Despite low sequence identity between these proteins, all of these structures have a similar fold. The crystal structure of the phiKZ tail sheath protein has been fitted into cryo-electron-microscopy reconstructions of the extended tail sheath and of a polysheath. The structural rearrangement of the phiKZ tail sheath contraction was found to be similar to that of phage T4. | |||
Structural conservation of the myoviridae phage tail sheath protein fold.,Aksyuk AA, Kurochkina LP, Fokine A, Forouhar F, Mesyanzhinov VV, Tong L, Rossmann MG Structure. 2011 Dec 7;19(12):1885-94. PMID:22153511<ref>PMID:22153511</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3spe" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas virus phiKZ]] | |||
[[Category: Aksyuk AA]] | |||
[[Category: Fokine A]] | |||
[[Category: Kurochkina LP]] | |||
[[Category: Mesyanzhinov VV]] | |||
[[Category: Rossmann MG]] | |||
Latest revision as of 12:41, 30 October 2024
Crystal structure of the tail sheath protein protease resistant fragment from bacteriophage phiKZCrystal structure of the tail sheath protein protease resistant fragment from bacteriophage phiKZ
Structural highlights
FunctionPublication Abstract from PubMedBacteriophage phiKZ is a giant phage that infects Pseudomonas aeruginosa, a human pathogen. The phiKZ virion consists of a 1450 A diameter icosahedral head and a 2000 A-long contractile tail. The structure of the whole virus was previously reported, showing that its tail organization in the extended state is similar to the well-studied Myovirus bacteriophage T4 tail. The crystal structure of a tail sheath protein fragment of phiKZ was determined to 2.4 A resolution. Furthermore, crystal structures of two prophage tail sheath proteins were determined to 1.9 and 3.3 A resolution. Despite low sequence identity between these proteins, all of these structures have a similar fold. The crystal structure of the phiKZ tail sheath protein has been fitted into cryo-electron-microscopy reconstructions of the extended tail sheath and of a polysheath. The structural rearrangement of the phiKZ tail sheath contraction was found to be similar to that of phage T4. Structural conservation of the myoviridae phage tail sheath protein fold.,Aksyuk AA, Kurochkina LP, Fokine A, Forouhar F, Mesyanzhinov VV, Tong L, Rossmann MG Structure. 2011 Dec 7;19(12):1885-94. PMID:22153511[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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