3soj: Difference between revisions

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-[[Image:3soj.jpg|left|200px]]
-<!--
+==Francisella tularensis pilin PilE==
-The line below this paragraph, containing "STRUCTURE_3soj", creates the "Structure Box" on the page.
+<StructureSection load='3soj' size='340' side='right'caption='[[3soj]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3soj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._tularensis_SCHU_S4 Francisella tularensis subsp. tularensis SCHU S4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SOJ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_3soj|  PDB=3soj  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3soj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3soj OCA], [https://pdbe.org/3soj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3soj RCSB], [https://www.ebi.ac.uk/pdbsum/3soj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3soj ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q5NGF6_FRATT Q5NGF6_FRATT]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Pilin proteins assemble into Type IV pili (T4P), surface-displayed bacterial filaments with virulence functions including motility, attachment, transformation, immune escape, and colony formation. However, challenges in crystallizing full-length fiber-forming and membrane protein pilins leave unanswered questions regarding pilin structures, assembly, functions, and vaccine potential. Here we report pilin structures of full-length DnFimA from the sheep pathogen Dichelobacter nodosus and FtPilE from the human pathogen Francisella tularensis at 2.3 and 1 A resolution, respectively. The DnFimA structure reveals an extended kinked N-terminal alpha-helix, an unusual centrally located disulfide, conserved subdomains, and assembled epitopes informing serogroup vaccines. An interaction between the conserved Glu-5 carboxyl oxygen and the N-terminal amine of an adjacent subunit in the crystallographic dimer is consistent with the hypothesis of a salt bridge between these groups driving T4P assembly. The FtPilE structure identifies an authentic Type IV pilin and provides a framework for understanding the role of T4P in F. tularensis virulence. Combined results define a unified pilin architecture, specialized subdomain roles in pilus assembly and function, and potential therapeutic targets.
-===Francisella tularensis pilin PilE===
+Ultrahigh Resolution and Full-length Pilin Structures with Insights for Filament Assembly, Pathogenic Functions, and Vaccine Potential.,Hartung S, Arvai AS, Wood T, Kolappan S, Shin DS, Craig L, Tainer JA J Biol Chem. 2011 Dec 23;286(51):44254-65. Epub 2011 Oct 24. PMID:22027840<ref>PMID:22027840</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3soj" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[3soj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._tularensis Francisella tularensis subsp. tularensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SOJ OCA].
+*[[Pilin 3D structures|Pilin 3D structures]]
-[[Category: Francisella tularensis subsp. tularensis]]
+== References ==
-[[Category: Arvai, A S.]]
+<references/>
-[[Category: Craig, L.]]
+__TOC__
-[[Category: Hartung, S.]]
+</StructureSection>
-[[Category: Kolappan, S.]]
+[[Category: Francisella tularensis subsp. tularensis SCHU S4]]
-[[Category: Shin, D S.]]
+[[Category: Large Structures]]
-[[Category: Tainer, J A.]]
+[[Category: Arvai AS]]
-[[Category: Wood, T.]]
+[[Category: Craig L]]
-[[Category: Cell adhesion]]
+[[Category: Hartung S]]
-[[Category: Extracellular]]
+[[Category: Kolappan S]]
-[[Category: Pilus subunit]]
+[[Category: Shin DS]]
+[[Category: Tainer JA]]
+[[Category: Wood T]]