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New page: '''Unreleased structure''' The entry 3s1s is ON HOLD Authors: Shen, B.W., Xu, D., Chan, S.-H., Zheng, Y., Zhu, Y., Xu, S.-Y., Stoddard, B.L. Description: Characterization and crystal s... |
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The | ==Characterization and crystal structure of the type IIG restriction endonuclease BpuSI== | ||
<StructureSection load='3s1s' size='340' side='right'caption='[[3s1s]], [[Resolution|resolution]] 2.35Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3s1s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_pumilus Bacillus pumilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S1S FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1s OCA], [https://pdbe.org/3s1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s1s RCSB], [https://www.ebi.ac.uk/pdbsum/3s1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s1s ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G1K3S1_BACPU G1K3S1_BACPU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A type IIG restriction endonuclease, RM.BpuSI from Bacillus pumilus, has been characterized and its X-ray crystal structure determined at 2.35A resolution. The enzyme is comprised of an array of 5-folded domains that couple the enzyme's N-terminal endonuclease domain to its C-terminal target recognition and methylation activities. The REase domain contains a PD-x(15)-ExK motif, is closely superimposable against the FokI endonuclease domain, and coordinates a single metal ion. A helical bundle domain connects the endonuclease and methyltransferase (MTase) domains. The MTase domain is similar to the N6-adenine MTase M.TaqI, while the target recognition domain (TRD or specificity domain) resembles a truncated S subunit of Type I R-M system. A final structural domain, that may form additional DNA contacts, interrupts the TRD. DNA binding and cleavage must involve large movements of the endonuclease and TRD domains, that are probably tightly coordinated and coupled to target site methylation status. | |||
Characterization and crystal structure of the type IIG restriction endonuclease RM.BpuSI.,Shen BW, Xu D, Chan SH, Zheng Y, Zhu Z, Xu SY, Stoddard BL Nucleic Acids Res. 2011 Jun 30. PMID:21724614<ref>PMID:21724614</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3s1s" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Endonuclease 3D structures|Endonuclease 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bacillus pumilus]] | |||
[[Category: Large Structures]] | |||
[[Category: Chan S-H]] | |||
[[Category: Shen BW]] | |||
[[Category: Stoddard BL]] | |||
[[Category: Xu D]] | |||
[[Category: Xu S-Y]] | |||
[[Category: Zheng Y]] | |||
[[Category: Zhu Y]] | |||