3r7b: Difference between revisions

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-[[Image:3r7b.png|left|200px]]
-{{STRUCTURE_3r7b|  PDB=3r7b  |  SCENE=  }}
+==Caspase-2 bound to one copy of Ac-DVAD-CHO==
+<StructureSection load='3r7b' size='340' side='right'caption='[[3r7b]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3r7b]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R7B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R7B FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ASA:ASPARTIC+ALDEHYDE'>ASA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r7b OCA], [https://pdbe.org/3r7b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r7b RCSB], [https://www.ebi.ac.uk/pdbsum/3r7b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r7b ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CASP2_HUMAN CASP2_HUMAN] Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Caspase-2, the most evolutionarily conserved member in the human caspase family, may play important roles in stress-induced apoptosis, cell cycle regulation, and tumor suppression. In biochemical assays, caspase-2 uniquely prefers a pentapeptide (such as VDVAD) rather than a tetrapeptide, as required for efficient cleavage by other caspases. We investigated the molecular basis for pentapeptide specificity using peptide analog inhibitors and substrates that vary at the P5 position. We determined the crystal structures of apo caspase-2, caspase-2 in complex with peptide inhibitors VDVAD-CHO, ADVAD-CHO, and DVAD-CHO, and a T380A mutant of caspase-2 in complex with VDVAD-CHO. Two residues, Thr-380 and Tyr-420, are identified to be critical for the P5 residue recognition; mutation of the two residues reduces the catalytic efficiency by about 4- and 40-fold, respectively. The structures also provide a series of snapshots of caspase-2 in different catalytic states, shedding light on the mechanism of capase-2 activation, substrate binding, and catalysis. By comparing the apo and inhibited caspase-2 structures, we propose that the disruption of a non-conserved salt bridge between Glu-217 and the invariant Arg-378 is important for the activation of caspase-2. These findings broaden our understanding of caspase-2 substrate specificity and catalysis.
-===Caspase-2 bound to one copy of Ac-DVAD-CHO===
+Structural and enzymatic insights into caspase-2 protein substrate recognition and catalysis.,Tang Y, Wells JA, Arkin MR J Biol Chem. 2011 Sep 30;286(39):34147-54. Epub 2011 Aug 2. PMID:21828056<ref>PMID:21828056</ref>
-{{ABSTRACT_PUBMED_21828056}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 3r7b" style="background-color:#fffaf0;"></div>
-[[3r7b]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R7B OCA].
 ==See Also==
-*[[Caspase|Caspase]]
+*[[Caspase 3D structures|Caspase 3D structures]]
+*[[Virus protease 3D structures|Virus protease 3D structures]]
-==Reference==
+== References ==
-<ref group="xtra">PMID:021828056</ref><references group="xtra"/>
+<references/>
-[[Category: Caspase-2]]
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Arkin, M.]]
+[[Category: Large Structures]]
-[[Category: Tang, Y.]]
+[[Category: Arkin M]]
-[[Category: Wells, J.]]
+[[Category: Tang Y]]
-[[Category: Apoptosis]]
+[[Category: Wells J]]
-[[Category: Hydrolase]]
-[[Category: Hydrolase-hydrolase inhibitor complex]]