2lag: Difference between revisions

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-[[Image:2lag.jpg|left|200px]]
-<!--
+==Structure of the 44 kDa complex of interferon-alpha2 with the extracellular part of IFNAR2 obtained by 2D-double difference NOESY==
-The line below this paragraph, containing "STRUCTURE_2lag", creates the "Structure Box" on the page.
+<StructureSection load='2lag' size='340' side='right'caption='[[2lag]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2lag]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LAG FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lag OCA], [https://pdbe.org/2lag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lag RCSB], [https://www.ebi.ac.uk/pdbsum/2lag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lag ProSAT]</span></td></tr>
-{{STRUCTURE_2lag|  PDB=2lag  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/INAR2_HUMAN INAR2_HUMAN] Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity.<ref>PMID:8181059</ref> <ref>PMID:7665574</ref> <ref>PMID:7759950</ref> <ref>PMID:11682488</ref> <ref>PMID:12105218</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+NMR detection of intermolecular interactions between protons in large protein complexes is very challenging since it is difficult to distinguish between weak NOEs from intermolecular interactions and the much larger number of strong intramolecular NOEs. This challenging task is exacerbated by the decrease in signal-to-noise ratio in the often used isotope-edited and isotope-filtered experiments as a result of enhanced T2 relaxation. Here we calculate a double difference spectrum that shows exclusively intermolecular NOEs and manifests the good signal-to-noise ratio in 2D homonuclear NOESY spectra even for large proteins. The method is straightforward and results in a complete picture of all intermolecular interactions involving non exchangeable protons. Ninety seven such 1H-1H NOEs were assigned for the 44 KDa interferon-alpha2/IFNAR2 complex and used for docking these two proteins. The symmetry of the difference spectrum, its superb resolution and unprecedented signal-to-noise ratio in this large protein/receptor complex suggest that this method is generally applicable to study large biopolymeric complexes.
-===Structure of the 44 kDa complex of interferon-alpha2 with the extracellular part of IFNAR2 obtained by 2D-double difference NOESY===
+Observation of intermolecular interactions in large protein complexes by 2D-double difference NOESY: application to the 44 kDa interferon-receptor complex.,Nudelman I, Akabayov SR, Scherf T, Anglister J J Am Chem Soc. 2011 Aug 8. PMID:21819146<ref>PMID:21819146</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2lag" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21819146}}, adds the Publication Abstract to the page
+*[[Interferon 3D structures|Interferon 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21819146 is the PubMed ID number.
+*[[Interferon receptor 3D structures|Interferon receptor 3D structures]]
--->
+*[[Multiple sclerosis|Multiple sclerosis]]
-{{ABSTRACT_PUBMED_21819146}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[2lag]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LAG OCA].
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:021819146</ref><references group="xtra"/>
 [[Category: Homo sapiens]]
-[[Category: Akabayov, S R.]]
+[[Category: Large Structures]]
-[[Category: Anglister, J.]]
+[[Category: Akabayov SR]]
-[[Category: Nudelman, I.]]
+[[Category: Anglister J]]
-[[Category: Scherf, T.]]
+[[Category: Nudelman I]]
-[[Category: Immune system]]
+[[Category: Scherf T]]
-[[Category: Interferon]]
-[[Category: Receptor]]