3quj: Difference between revisions

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-[[Image:3quj.png|left|200px]]
-<!--
+==Crystal structure of the phosphonate binding protein, PhnD, from Escherichia coli==
-The line below this paragraph, containing "STRUCTURE_3quj", creates the "Structure Box" on the page.
+<StructureSection load='3quj' size='340' side='right'caption='[[3quj]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3quj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_UTI89 Escherichia coli UTI89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QUJ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-{{STRUCTURE_3quj|  PDB=3quj  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3quj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3quj OCA], [https://pdbe.org/3quj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3quj RCSB], [https://www.ebi.ac.uk/pdbsum/3quj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3quj ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q1R3F7_ECOUT Q1R3F7_ECOUT]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The phnD gene of Escherichia coli encodes the periplasmic binding protein of the phosphonate (Pn) uptake and utilization pathway. We have crystallized and determined structures of E. coli PhnD (EcPhnD) in the absence of ligand and in complex with the environmentally abundant 2-aminoethylphosphonate (2AEP). Similar to other bacterial periplasmic binding proteins, 2AEP binds near the center of mass of EcPhnD in a cleft formed between two lobes. Comparison of the open, unliganded structure with the closed 2AEP-bound structure shows that the two lobes pivot around a hinge by approximately 70 degrees between the two states. Extensive hydrogen bonding and electrostatic interactions stabilize 2AEP, which binds to EcPhnD with low nanomolar affinity. These structures provide insight into Pn uptake by bacteria and facilitated the rational design of high signal-to-noise Pn biosensors based on both coupled small-molecule dyes and autocatalytic fluorescent proteins.
-===Crystal structure of the phosphonate binding protein, PhnD, from Escherichia coli===
+Structure of the Escherichia coli Phosphonate Binding Protein PhnD and Rationally Optimized Phosphonate Biosensors.,Alicea I, Marvin JS, Miklos AE, Ellington AD, Looger LL, Schreiter ER J Mol Biol. 2011 Dec 2;414(3):356-69. Epub 2011 Oct 12. PMID:22019591<ref>PMID:22019591</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3quj" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[3quj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_uti89 Escherichia coli uti89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUJ OCA].
+*[[ABC transporter 3D structures|ABC transporter 3D structures]]
-[[Category: Escherichia coli uti89]]
+== References ==
-[[Category: Alicea, I.]]
+<references/>
-[[Category: Schreiter, E R.]]
+__TOC__
-[[Category: Bind and transport of phosphonate]]
+</StructureSection>
-[[Category: Transport protein]]
+[[Category: Escherichia coli UTI89]]
+[[Category: Large Structures]]
+[[Category: Alicea I]]
+[[Category: Schreiter ER]]