3q1s: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 3q1s is ON HOLD
+==HIV-1 neutralizing antibody Z13e1 in complex with epitope display protein==
+<StructureSection load='3q1s' size='340' side='right'caption='[[3q1s]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3q1s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q1S FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q1s OCA], [https://pdbe.org/3q1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q1s RCSB], [https://www.ebi.ac.uk/pdbsum/3q1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q1s ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IL22_HUMAN IL22_HUMAN] Cytokine that contributes to the inflammatory response in vivo.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Antibody Z13e1 is a relatively broadly neutralizing anti-human immunodeficiency virus type 1 antibody that recognizes the membrane-proximal external region (MPER) of the human immunodeficiency virus type 1 envelope glycoprotein gp41. Based on the crystal structure of an MPER epitope peptide in complex with Z13e1 Fab, we identified an unrelated protein, interleukin (IL)-22, with a surface-exposed region that is structurally homologous in its backbone to the gp41 Z13e1 epitope. By grafting the gp41 Z13e1 epitope sequence onto the structurally homologous region in IL-22, we engineered a novel protein (Z13-IL22-2) that contains the MPER epitope sequence for use as a potential immunogen and as a reagent for the detection of Z13e1-like antibodies. The Z13-IL22-2 protein binds Fab Z13e1 with a K(d) of 73 nM. The crystal structure of Z13-IL22-2 in complex with Fab Z13e1 shows that the epitope region is faithfully replicated in the Fab-bound scaffold protein; however, isothermal calorimetry studies indicate that Fab binding to Z13-IL22-2 is not a lock-and-key event, leaving open the question of whether conformational changes upon binding occur in the Fab, in Z13-IL-22, or in both.
-Authors: Stanfield, R.L., Pejchal, R., Gach, J.S., Zwick, M.B., Wilson, I.A.
+Structure-Based Design of a Protein Immunogen that Displays an HIV-1 gp41 Neutralizing Epitope.,Stanfield RL, Julien JP, Pejchal R, Gach JS, Zwick MB, Wilson IA J Mol Biol. 2011 Dec 2;414(3):460-76. Epub 2011 Oct 15. PMID:22033480<ref>PMID:22033480</ref>
-Description: HIV-1 neutralizing antibody Z13e1 in complex with epitope display protein
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3q1s" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Interleukin 3D structures|Interleukin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Gach JS]]
+[[Category: Julien J-P]]
+[[Category: Pejchal R]]
+[[Category: Stanfield RL]]
+[[Category: Wilson IA]]
+[[Category: Zwick MB]]