2y1s: Difference between revisions

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-[[Image:2y1s.jpg|left|200px]]
-<!--
+==Microvirin lectin==
-The line below this paragraph, containing "STRUCTURE_2y1s", creates the "Structure Box" on the page.
+<StructureSection load='2y1s' size='340' side='right'caption='[[2y1s]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2y1s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Microcystis_aeruginosa Microcystis aeruginosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y1S FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 40 models</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y1s OCA], [https://pdbe.org/2y1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y1s RCSB], [https://www.ebi.ac.uk/pdbsum/2y1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y1s ProSAT]</span></td></tr>
-{{STRUCTURE_2y1s|  PDB=2y1s  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q2MDE2_MICA7 Q2MDE2_MICA7]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Lectins that bind surface envelope glycoprotein gp120 of HIV with high avidity can potently inhibit viral entry. Yet properties such as multivalency that facilitate strong interactions can also cause nonspecific binding and toxicity. The cyanobacterial lectin microvirin (MVN) is unusual as it potently inhibits HIV-1 with negligible toxicity compared with cyanovirin-N (CVN), its well studied antiviral homolog. To understand the structural and mechanistic basis for these differences, we solved the solution structure of MVN free and in complex with its ligand Manalpha(1-2)Man, and we compared specificity and time windows of inhibition with CVN and Manalpha(1-2)Man-specific mAb 2G12. We show by NMR and analytical ultracentrifugation that MVN is monomeric in solution, and we demonstrate by NMR that Manalpha(1-2)Man-terminating carbohydrates interact with a single carbohydrate-binding site. Synchronized infectivity assays show that 2G12, MVN, and CVN inhibit entry with distinct kinetics. Despite shared specificity for Manalpha(1-2)Man termini, combinations of the inhibitors are synergistic suggesting they recognize discrete glycans and/or dynamic glycan conformations on gp120. Entry assays employing amphotropic viruses show that MVN is inactive, whereas CVN potently inhibits both. In addition to demonstrating that HIV-1 can be inhibited through monovalent interactions, given the similarity of the carbohydrate-binding site common to MVN and CVN, these data suggest that gp120 behaves as a clustered glycan epitope and that multivalent-protein interactions achievable with CVN but not MVN are required for inhibition of some viruses.
-===MICROVIRIN LECTIN===
+Solution structure of the monovalent lectin microvirin in complex with Man(alpha)(1-2)Man provides a basis for anti-HIV activity with low toxicity.,Shahzad-ul-Hussan S, Gustchina E, Ghirlando R, Clore GM, Bewley CA J Biol Chem. 2011 Jun 10;286(23):20788-96. Epub 2011 Apr 6. PMID:21471192<ref>PMID:21471192</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2y1s" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_20507987}}, adds the Publication Abstract to the page
+*[[Mannose-binding protein|Mannose-binding protein]]
-(as it appears on PubMed at http://www.pubmed.gov), where 20507987 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_20507987}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-[[2y1s]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y1S OCA].
+[[Category: Microcystis aeruginosa]]
+[[Category: Bewley CA]]
-==Reference==
+[[Category: Hussan S]]
-<ref group="xtra">PMID:20507987</ref><references group="xtra"/>
-[[Category: Bewley, C.]]
-[[Category: Hussan, S.]]