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| <StructureSection load='' size='350' side='right' caption='Subtilisin (deepskyblue) complex with streptomycin inhibitor (green) and Ca+2 ions (green) (PDB entry [[2sic]])' scene='43/430882/Cv/2'> | | <StructureSection load='' size='350' side='right' caption='Subtilisin (deepskyblue) complex with streptomycin inhibitor (green) and Ca+2 ions (green) (PDB entry [[2sic]])' scene='43/430882/Cv/2'> |
| == Function == | | == Function == |
| [[Subtilisin]] is a serine protease. A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzym<ref>PMID:4967581</ref>. See detalis in [[User:Tommie Hata/Introduction to Protein Engineering-Subtilisin]]. | | [[Subtilisin]] is a serine protease. A 106 amino acid propeptide is cleaved from the N-terminus of '''pro-subtilisin''' to create the '''mature''' active enzym<ref>PMID:4967581</ref>. See detalis in [[User:Tommie Hata/Introduction to Protein Engineering-Subtilisin]]. |
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| *'''Selenosubtilisin''' is a semisynthetic selenoenzyme produced by chemical modification of subtilisin<ref>PMID:8385489</ref>.<br /> | | *'''Selenosubtilisin''' is a semisynthetic selenoenzyme produced by chemical modification of subtilisin<ref>PMID:8385489</ref>.<br /> |
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| </StructureSection> | | </StructureSection> |
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| == 3D Structures of Subtilisin ==
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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| {{#tree:id=OrganizedByTopic|openlevels=0|
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| * Native mature subtilisin
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| **[[2z2x]] – TkSub - ''Thermococcus kodakarensis''<br />
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| **[[1sca]], [[1scb]], [[1sbc]], [[3unx]] – BsSub - ''Bacillus subtilis'' (Subtilisin Carlsberg)<br />
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| **[[6pak]] - BsSub E (mutant)<br />
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| **[[1pxq]] – BsSub - NMR<br />
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| **[[1gns]], [[1sup]], [[1st2]], [[1s01]], [[1sbt]], [[2sbt]], [[2st1]] – BaSub - ''Bacillus amyloliquefaciens''<br />
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| **[[1ea7]], [[3d43]], [[2ixt]] – spehricase – Sub from ''Bacillus sphaericus''<br />
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| **[[2gko]], [[3vyv]], [[4dww]] – BsSub – ''Bacillus subtilis''
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| * Mutant mature subtilisin
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| **[[3f49]], [[3co0]], [[2zrq]], [[3vhq]] – TkSub (mutant) <br />
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| **[[1gnv]], [[1dui]], [[1sue]], [[1a2q]], [[1aqn]], [[1sua]], [[1au9]], [[1ak9]], [[1yja]], [[1yjb]], [[1yjc]], [[1sbh]], [[1sbi]], [[1sub]], [[1suc]], [[1sud]], [[1s02]] – BaSub (mutant)<br />
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| **[[2xrm]] - BcSub residues 19-321 - ''Bacillus clausii''<br />
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| * Unautoprocessed subtilisin (pro-Sub)
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| **[[2z2z]], [[2e1p]] – pro-TkSub <br />
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| **[[2zwo]], [[2zwp]], [[4jp8]], [[3wiu]], [[3wiv]] – pro-TKSub (mutant)<br />
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| **[[2wv7]] – pro-BcSub <br />
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| **[[2wwt]], [[2x8j]] - pro-BcSub (mutant)<br />
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| **[[3whi]] - pro-BcSub (mutant)<br />
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| * Mature subtilisin complexed with its propeptide
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| **[[3cnq]], [[5ox2]] – BaSub + prodomain<br />
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| **[[1spb]] – BaSub (mutant) + prodomain<br />
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| **[[3a3n]], [[3a3o]], [[3a3p]], [[2z56]], [[2z57]], [[2z58]], [[2z2y]], [[2z30]] – TkSub + prodomain<br />
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| **[[3vv2]] – TkSub (mutant) + prodomain<br />
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| **[[1scj]] – BsSub + prodomain<br />
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| * Subtilisin complexed with polypeptide inhibitor
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| **[[1y1k]], [[1y33]], [[1y34]], [[1y3b]], [[1y3c]], [[1y3d]], [[1y3f]], [[1y48]], [[1y4a]], [[1y4d]], [[1tm3]], [[1tm4]],
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| **[[1tm1]], [[1lw6]] – BaSub+ chymotripsin inhibitor 2<br />
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| **[[1tm5]], [[1tm7]], [[1tmg]], [[1to1]], [[1to2]] - BaSub+ chymotrypsin inhibitor 2 (mutant)<br />
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| **[[3sic]], [[5sic]], [[2sic]] – BaSub+Streptomyces inhibitor SSI<br />
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| **[[1yu6]] – BsSub+ovomucoid <br />
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| **[[1v5i]] – BaSubb+POIA1 peptide <br />
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| **[[1r0r]] – BsSub+ovomucoid<br />
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| **[[1oyv]] – BsSub+tomato inhibitor II<br />
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| **[[1sbn]], [[1sib]], [[2sec]], [[1cse]] – BsSub+Elgin C<br />
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| **[[2sni]] - BaSub+Elgin C2 <br />
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| **[[1mee]] - BpSub+Elgin C – ''Bacillus pumilus''
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| * Subtilisin complexed with inhibitor
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| **[[3bgo]] – BaSub+azide <br />
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| **[[1c3l]] – BsSub+Xe<br />
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| **[[1be8]] – BsSub+cinnamoyl<br />
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| **[[1be6]] - BsSub+cinnamoyl+acetonitrile<br />
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| **[[1bfk]], [[1scd]] – BsSub+acetonitrile<br />
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| **[[1bfu]], [[1af4]] – BsSub+dioxane<br />
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| **[[1av7]], [[1avt]], [[3vsb]], [[1vsb]] – BsSub+boronic acid derivatives <br />
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| **[[1scn]] – BsSub+carbamate derivative <br />
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| **[[1bh6]] – BliSub DY+benzyloxycarbonyl-ala-pro-phe-chloromethyl ketone – ''Bacillus licheniformis''<br />
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| **[[2wuv]], [[2wuw]] – BliSub fragment+acetonitrile<br />
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| **[[4c3u]] – BliSub + Ca + Cs<br />
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| **[[4c3v]] – BliSub + Ca <br />
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| * Selenosubtilisin
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| **[[1ubn]] – BaSel-Sub<br />
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| **[[1sel]] – BsSel-Sub
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| * Savinase
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| **[[3bx1]], [[1svn]], [[4cfy]], [[4cfz]], [[4cg0]], [[1iav]], [[1gci]], [[1jea]], [[1st3]], [[5aqe]], [[5arb]], [[5arc]], [[5ard]] – BlSav – ''Bacillus lentus''<br />
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| **[[1ndu]], [[1q5p]], [[1c9j]], [[1c9m]], [[1c9n]] – BlSav (mutant)<br />
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| **[[1tk2]], [[1ndq]] – BlSav+gramicidin S <br />
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| **[[4hx2]] – BlSav + Ca + Zn + sermetstatin<br />
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |