Pepsin: Difference between revisions
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<StructureSection load='5pep' size='350' side='right' scene='Sandbox_179/Default/1' caption='Pig pepsin (PDB code [[5pep]])'> | <StructureSection load='5pep' size='350' side='right' scene='Sandbox_179/Default/1' caption='Pig pepsin (PDB code [[5pep]])'> | ||
==Introduction== | |||
Theodor Schwann discovered [[Pepsin]] in 1836. He named the enzyme pepsis from the Greek word peptein which means to digest. Pepsin was the first animal enzyme to be discovered, and, in 1929, it became one of the first enzymes to be crystallized, by John H. Northrop. | Theodor Schwann discovered [[Pepsin]] in 1836. He named the enzyme pepsis from the Greek word peptein which means to digest. Pepsin was the first animal enzyme to be discovered, and, in 1929, it became one of the first enzymes to be crystallized, by John H. Northrop. | ||
Pepsin was also the first crystallized protein to be studied by X-ray diffraction using the method of capillary mounting to prevent water loss <ref name="Xray">PMID: 2115088</ref>. | Pepsin was also the first crystallized protein to be studied by X-ray diffraction using the method of capillary mounting to prevent water loss <ref name="Xray">PMID: 2115088</ref>. | ||
*'''Pepsinogen''' is the inactive precursor of pepsin. It is converted to the active pepsin by the hydrochloric acid in the stomach<ref>PMID:8260073</ref>. | |||
See also [[Pepsin (Hebrew)]] | *'''Endothiapepsin''' is pepsin from ''Endothia parasitica'' which is used for fragment screening<ref>PMID:27400756</ref>. | ||
See also: | |||
*[[Pepsin (Hebrew)]] | |||
*[[Proteinase]] | |||
==Protein Function== | ==Protein Function== | ||
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*'''Pepsin''' | *'''Pepsin''' | ||
**[[1flh]], [[1psn]], [[3utl]] – hPep – human<br /> | |||
**[[5pep]], [[3pep]], [[4pep]] – pPep – pig<br /> | |||
**[[1am5]] – Pep – Atlantic cod<br /> | |||
**[[1fmu]], [[1fmx]], [[2jxr]] – yPep – yeast<br /> | |||
**[[2h6s]], [[2qzw]] – CaPep residues 59-398 – ''Candida albicans''<br /> | **[[2h6s]], [[2qzw]] – CaPep residues 59-398 – ''Candida albicans''<br /> | ||
**[[1s2b]] – SlPep - ''Scytalidium lignicola''<br /> | **[[1s2b]] – SlPep - ''Scytalidium lignicola''<br /> | ||
**[[1y43]] – Pep – ''Aspergillus niger''<br /> | **[[1y43]] – Pep – ''Aspergillus niger''<br /> | ||
**[[1ibq]] - Pep – ''Aspergillus phoenicis''<br /> | **[[1ibq]] - Pep – ''Aspergillus phoenicis''<br /> | ||
**[[1uh7]], [[1uh8]], [[1uh9]], [[2apr]] – RcPep – ''Rhizopus chinensis''<br /> | **[[1uh7]], [[1uh8]], [[1uh9]], [[2apr]] – RcPep – ''Rhizopus chinensis''<br /> | ||
**[[1mpp]] – Pep – ''Rhizomucor pusillus''<br /> | **[[1mpp]] – Pep – ''Rhizomucor pusillus''<br /> | ||
**[[3app]] – PjPep – ''Penicillium janthinellum''<br /> | **[[3app]] – PjPep – ''Penicillium janthinellum''<br /> | ||
**[[1er8]], [[3er3]], [[4er1]], [[4ape]], [[1ent]] – CpPep – ''Cryphonectria parasitica''<br /> | **[[1er8]], [[3er3]], [[4er1]], [[4ape]], [[1ent]] – CpPep – ''Cryphonectria parasitica''<br /> | ||
**[[3lzy]], [[1oew]] – CpPep residues 90-419 | **[[3lzy]], [[1oew]] – CpPep residues 90-419 | ||
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*Pepsin complex with inhibitor | *Pepsin complex with inhibitor | ||
**[[1qrp]] – hPep + peptide<br /> | |||
**[[1pso]] – hPep + pepstatin<br /> | |||
**[[1yx9]] – pPep + DMSO<br /> | |||
**[[1psa]], [[1f34]] – pPep + inhibitor<br /> | |||
**[[6xct]], [[6xcy]], [[6xcz]], [[6xd2]] – pPep + drug<br /> | |||
**[[1g0v]] – yPep + protease A inhibitor<br /> | |||
**[[1fq4]], [[1fq5]], [[1fq6]], [[1fq7]], [[1fq8]] - yPep + inhibitor<br /> | |||
**[[2h6t]] - CaPep residues 59-398 + pepstatin<br /> | **[[2h6t]] - CaPep residues 59-398 + pepstatin<br /> | ||
**[[1eag]] - CaPep residues 59-398 + inhibitor<br /> | **[[1eag]] - CaPep residues 59-398 + inhibitor<br /> | ||
**[[2qzx]] - CaPep residues 77-418 + pepstatin<br /> | **[[2qzx]] - CaPep residues 77-418 + pepstatin<br /> | ||
**[[1j71]] - Pep + peptide – ''Candida tropicalis | **[[1j71]] - Pep + peptide – ''Candida tropicalis''<br /> | ||
**[[2ifr]], [[2ifw]], [[1s2k]] – SlPep + peptide<br /> | **[[2ifr]], [[2ifw]], [[1s2k]] – SlPep + peptide<br /> | ||
**[[1wkr]] - Pep + pepstatin – ''Irpex lacteus''<br /> | **[[1wkr]] - Pep + pepstatin – ''Irpex lacteus''<br /> | ||
**[[4apr]], [[5apr]], [[6apr]] - RcPep + pepstatin<br /> | **[[4apr]], [[5apr]], [[6apr]] - RcPep + pepstatin<br /> | ||
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**[[2rmp]] - Pep + pepstatin – ''Rhizomucor miehei''<br /> | **[[2rmp]] - Pep + pepstatin – ''Rhizomucor miehei''<br /> | ||
**[[1nlu]], [[1ga1]], [[1ga4]] - PsPep + pseudo-iodotyrostatin – ''Pseudomonas''<br /> | **[[1nlu]], [[1ga1]], [[1ga4]] - PsPep + pseudo-iodotyrostatin – ''Pseudomonas''<br /> | ||
**[[ | **[[6m8w]], [[6m8y]] – PsPep + peptide<br /> | ||
**[[ | **[[6m8f]], [[6m9c]], [[6m9d]], [[1ga6]] - PsPep + statin inhibitor<br /> | ||
**[[1bxo]], [[1bxq]], [[2wea]], [[2web]], [[2wec]], [[2wed]] - PjPep + inhibitor <br /> | **[[1bxo]], [[1bxq]], [[2wea]], [[2web]], [[2wec]], [[2wed]] - PjPep + inhibitor <br /> | ||
**[[1ppk]], [[1apv]], [[1apw]], [[1ppl]], [[1ppm]] – PjPep + peptide analog | **[[1ppk]], [[1apv]], [[1apw]], [[1ppl]], [[1ppm]] – PjPep + peptide analog | ||
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*'''Pepsinogen''' | *'''Pepsinogen''' | ||
**[[3psg]], [[2psg]] - pPepn<br /> | **[[1avf]] - hPepn<br /> | ||
**[[ | **[[1yx9]], [[3psg]], [[2psg]] - pPepn<br /> | ||
**[[6row]] – Pepn + cysteine protease + zinc metallopeptidase – worm – Cryo EM<br /> | |||
*'''Endothiapepsin''' | *'''Endothiapepsin or aspartic proteinase''' | ||
**[[1er8]], [[3er3]], [[4er1]], [[4ape]], [[1ent]] – CpPep – ''Cryphonectria parasitica''<br /> | **[[1er8]], [[3er3]], [[4er1]], [[4ape]], [[1ent]], [[5qbk]], [[5qbq]], [[5qbr]], [[5qbs]], [[5qbt]] – CpPep – ''Cryphonectria parasitica''<br /> | ||
**[[5rci]], [[5rcj]], [[5rck]], [[5rcn]], [[5rco]], [[5rcp]], [[5rcr]], [[5rct]], [[5rcu]], [[5rcw]], [[5rcy]], [[5rd0]], [[5rd1]], [[5rd3]], [[5rd5]], [[5rd6]], [[5rd8]], [[5rdb]], [[5rdc]], [[5rdd]], [[5rdf]], [[5rdj]], [[5rdk]], [[5rdl]], [[5rdn]], [[5re3]] – CpETPep ground state<br /> | |||
**[[3lzy]], [[1oew]] – CpPep residues 90-419<br /> | **[[3lzy]], [[1oew]] – CpPep residues 90-419<br /> | ||
**[[3pb5]], [[3pbd]], [[3pbz]], [[3pcw]], [[3pgi]], [[3pi0]], [[3pld]], [[3pll]], [[3pm4]], [[3pmu]], [[3pmy]] – CpETPep + fragment chemotype<br /> | **[[3pb5]], [[3pbd]], [[3pbz]], [[3pcw]], [[3pgi]], [[3pi0]], [[3pld]], [[3pll]], [[3pm4]], [[3pmu]], [[3pmy]] – CpETPep + fragment chemotype<br /> | ||
**[[3pcz]] – CpETPep + benzamidine<br /> | **[[3pcz]] – CpETPep + benzamidine<br /> | ||
**[[3prs]], [[3pww]] – CpETPep + antiviral drug<br /> | **[[3prs]], [[3pww]] – CpETPep + antiviral drug<br /> | ||
**[[3q6y]], [[1gvx]], [[1gvu]], [[1gvv]], [[1gvw]], [[1gvt]], [[2jji]], [[2jjj]] - CpETPep + pyrrolidine derivative<br /> | **[[3q6y]], [[1gvx]], [[1gvu]], [[1gvv]], [[1gvw]], [[1gvt]], [[2jji]], [[2jjj]] - CpETPep + pyrrolidine derivative<br /> | ||
**[[3t6i]] - CpETPep + azepine derivative<br /> | **[[5qbj]], [[5qbn]], [[5qbo]], [[5qbp]] - CpETPep + piperidine derivative<br /> | ||
**[[3t7p]], [[4lhh]], [[4lbt]], [[4kup]] - CpETPep + hydrazine derivative<br /> | **[[5qbh]], [[5qbi]], [[5qbm]] - CpETPep + pyridine derivative<br /> | ||
**[[4l6b]], [[3t7x]], [[3t7q]], [[3psy]] – CpETPwo + thiophen inhibitor<br /> | **[[5qbl]] - CpETPep + morpholine derivative<br /> | ||
**[[3t6i]], [[5qbg]] - CpETPep + azepine derivative<br /> | |||
**[[3t7p]], [[4lhh]], [[4lbt]], [[4kup]], [[5hco]], [[5hct]], [[5og7]] - CpETPep + hydrazine derivative<br /> | |||
**[[4l6b]], [[3t7x]], [[3t7q]], [[3psy]], [[4lap]] – CpETPwo + thiophen inhibitor<br /> | |||
**[[3er5]], [[4er4]], [[5er2]], [[2er0]], [[2er6]], [[2er7]], [[2er9]], [[5er1]], [[1epl]], [[1epm]], [[1epn]], [[1epo]], [[1epp]], [[1epq]], [[1epr]], [[3uri]], [[3url]] – CpETPep + peptide<br /> | **[[3er5]], [[4er4]], [[5er2]], [[2er0]], [[2er6]], [[2er7]], [[2er9]], [[5er1]], [[1epl]], [[1epm]], [[1epn]], [[1epo]], [[1epp]], [[1epq]], [[1epr]], [[3uri]], [[3url]] – CpETPep + peptide<br /> | ||
**[[4er2]] - CpETPep + pepstatin<br /> | **[[4er2]] - CpETPep + pepstatin<br /> | ||
**[[2v00]], [[1od1]], [[1oex]], [[1eed]], [[1e81]], [[1e82]], [[1e80]], [[1e5o]] – CpETPep + inhibitor<br /> | **[[2v00]], [[1od1]], [[1oex]], [[1eed]], [[1e81]], [[1e82]], [[1e80]], [[1e5o]], [[3wz6]], [[3wz7]], [[3wz8]], [[4y35]], [[4y36]], [[4y37]], [[4y38]], [[4y39]], [[4y3a]], [[4y3d]], [[4y3e]], [[4y3f]], [[4y3g]], [[4y3h]], [[4y3j]], [[4y3l]], [[4y3m]], [[4y3n]], [[4y3p]], [[4y3q]], [[4y3r]], [[4y3s]], [[4y3t]], [[4y3w]], [[4y3x]], [[4y3y]], [[4y3z]], [[4y41]], [[4y43]], [[4y44]], [[4y45]], [[4y47]], [[4y48]], [[4y4a]], [[4y4b]], [[4y4d]], [[4y4e]], [[4y4g]], [[4y4j]], [[4y4t]], [[4y4u]], [[4y4w]], [[4y4x]], [[4y4z]], [[4y50]], [[4y51]], [[4y53]], [[4y54]], [[4y56]], [[4y57]], [[4y58]], [[4y5a]], [[4y5b]], [[4y5c]], [[4y5e]], [[4y5g]], [[4y5k]], [[4y5l]], [[4y5m]], [[4y5n]], [[4y5p]], [[4yck]], [[4yct]], [[4ycy]], [[4yd3]], [[4yd4]], [[4yd5]], [[4yd6]], [[4yd7]], [[4ze6]], [[4zea]], [[5dpz]], [[5dq1]], [[5dq2]], [[5dq4]], [[5dq5]], [[5dr0]], [[5dr1]], [[5dr3]], [[5dr4]], [[5dr7]], [[5dr8]], [[5is4]], [[5isj]], [[5isk]], [[5j25]], [[5lwr]], [[5lws]], [[5lwt]], [[5lwu]], [[5mb0]], [[5mb3]], [[5mb5]], [[5mb6]], [[5mb7]], [[5oje]], [[5oyq]], [[5oyr]], [[5oys]], [[5oyt]], [[5oyu]], [[5oyv]], [[5oyw]], [[5oyx]], [[5oyy]], [[5oyz]], [[5oz0]], [[5oz1]], [[5oz2]], [[5oz3]], [[5oz4]], [[5oz5]], [[5oz6]], [[5oz7]], [[5oz8]], [[5oz9]], [[5oza]], [[5ozb]], [[5ozc]], [[5ozd]], [[5oze]], [[5ozf]], [[5ozg]], [[5ozh]], [[5ozi]], [[5ozj]], [[5ozk]], [[5ozl]], [[5ozm]], [[5ozn]], [[5ozo]], [[5ozp]], [[5ozq]], [[5ozr]], [[5ozs]], [[5ozt]], [[5ozu]], [[5ozv]], [[5ozw]], [[5ozx]], [[5ozy]], [[5ozz]], [[5p00]], [[5p01]], [[5p02]], [[5p03]], [[5p04]], [[5p05]], [[5p06]], [[5p07]], [[5p08]], [[5p09]], [[5p0a]], [[5p0b]], [[5p0c]], [[5p0d]], [[5p0e]], [[5p0f]], [[5p0g]], [[5p0h]], [[5p0i]], [[5p0j]], [[5p0k]], [[5p0l]], [[5p0m]], [[5p0n]], [[5p0o]], [[5p0p]], [[5p0q]], [[5p0r]], [[5p0s]], [[5p0t]], [[5p0u]], [[5p0v]], [[5p0w]], [[5p0x]], [[5p0y]], [[5p0z]], [[5p10]], [[5p11]], [[5p12]], [[5p13]], [[5p14]], [[5p15]], [[5p16]], [[5p17]], [[5p18]], [[5p19]], [[5p1a]], [[5p1b]], [[5p1c]], [[5p1d]], [[5p1e]], [[5p1f]], [[5p1g]], [[5p1h]], [[5p1i]], [[5p1j]], [[5p1k]], [[5p1l]], [[5p1m]], [[5p1n]], [[5p1o]], [[5p1p]], [[5p1q]], [[5p1r]], [[5p1s]], [[5p1t]], [[5p1u]], [[5p1v]], [[5p1w]], [[5p1x]], [[5p1y]], [[5p1z]], [[5p20]], [[5p22]], [[5p23]], [[5p24]], [[5p25]], [[5p26]], [[5p27]], [[5p28]], [[5p29]], [[5p2a]], [[5p2b]], [[5p2c]], [[5p2d]], [[5p2e]], [[5p2f]], [[5p2g]], [[5p2h]], [[5p2i]], [[5p2j]], [[5p2k]], [[5p2l]], [[5p2m]], [[5p2n]], [[5p2o]], [[5p2q]], [[5p2r]], [[5p2s]], [[5p2t]], [[5p2u]], [[5p2v]], [[5p2w]], [[5p2x]], [[5p2y]], [[5p2z]], [[5p30]], [[5p31]], [[5p32]], [[5p33]], [[5p34]], [[5p35]], [[5p36]], [[5p37]], [[5p38]], [[5p39]], [[5p3a]], [[5p3b]], [[5p3c]], [[5p3d]], [[5p3e]], [[5p3f]], [[5p3g]], [[5p3h]], [[5p3i]], [[5p3j]], [[5p3k]], [[5p3l]], [[5p3m]], [[5p3n]], [[5p3o]], [[5p3p]], [[5p3q]], [[5p3r]], [[5p3s]], [[5p3t]], [[5p3u]], [[5p3v]], [[5p3w]], [[5p3x]], [[5p3y]], [[5p3z]], [[5p40]], [[5p41]], [[5p42]], [[5p43]], [[5p44]], [[5p45]], [[5p46]], [[5p47]], [[5p48]], [[5p49]], [[5p4a]], [[5p4b]], [[5p4c]], [[5p4d]], [[5p4e]], [[5p4f]], [[5p4g]], [[5p4h]], [[5p4i]], [[5p4j]], [[5p4k]], [[5p4l]], [[5p4m]], [[5p4n]], [[5p4o]], [[5p4p]], [[5p4q]], [[5p4r]], [[5p4s]], [[5p4t]], [[5p4u]], [[5p4v]], [[5p4w]], [[5p4x]], [[5p4y]], [[5p4z]], [[5p50]], [[5p51]], [[5p52]], [[5p53]], [[5p54]], [[5p55]], [[5p56]], [[5p57]], [[5p58]], [[5p59]], [[5p5a]], [[5p5b]], [[5p5c]], [[5p5d]], [[5p5e]], [[5p5f]], [[5p5g]], [[5p5h]], [[5p5i]], [[5p5j]], [[5p5k]], [[5p5l]], [[5p5m]], [[5p5n]], [[5p5o]], [[5p5p]], [[5p5q]], [[5p5r]], [[5p5s]], [[5p5t]], [[5p5u]], [[5p5v]], [[5p5w]], [[5p5x]], [[5p5y]], [[5p5z]], [[5p60]], [[5p61]], [[5p62]], [[5p63]], [[5p64]], [[5p65]], [[5p66]], [[5p67]], [[5p68]], [[5p69]], [[5p6a]], [[5p6b]], [[5p6c]], [[5p6d]], [[5p6e]], [[5p6f]], [[5p6g]], [[5p6h]], [[5p6i]], [[5p6j]], [[5p6k]], [[5p6l]], [[5p6m]], [[5p6n]], [[5p6o]], [[5p6p]], [[5p6q]], [[5p6r]], [[5p6s]], [[5p6t]], [[5p6u]], [[5p6v]], [[5p6w]], [[5p6x]], [[5p6y]], [[5p6z]], [[5p70]], [[5p71]], [[5p72]], [[5p73]], [[5p74]], [[5p75]], [[5p76]], [[5p77]], [[5p78]], [[5p79]], [[5p7a]], [[5p7b]], [[5p7c]], [[5p7d]], [[5p7e]], [[5p7f]], [[5p7g]], [[5p7h]], [[5p7i]], [[5p7j]], [[5p7k]], [[5p7l]], [[5p7m]], [[5p7n]], [[5p7o]], [[5p7p]], [[5p7q]], [[5p7r]], [[5p7s]], [[5p7t]], [[5p7u]], [[5p7v]], [[5p7w]], [[5p7x]], [[5p7y]], [[5p7z]], [[5p80]], [[5p81]], [[5p82]], [[5p83]], [[5p84]], [[5p85]], [[5p86]], [[5p87]]. [[5p88]], [[5p89]], [[5p8a]]. [[5p8b]], [[5p8c]], [[5p8d]]. [[5p8e]], [[5p8f]], [[5p8g]]. [[5p8h]], [[5p8i]], [[5p8j]]. [[5p8k]], [[5p8l]], [[5p8m]], [[5p8n]], [[5p8o]], [[5p8p]]. [[5p8q]], [[5p8r]], [[5p8s]]. [[5p8t]], [[5p8u]], [[5p8v]], [[5qb5]], [[5qb6]], [[5qb7]], [[5qb8]], [[5qb9]], [[5qba]], [[5qbb]], [[5qbc]], [[5qbd]], [[5qbe]], [[5qbf]], [[5r1t]], [[5r1u]], [[5r1v]], [[5r1w]], [[5r1x]], [[5r1y]], [[5r1z]], [[5r20]], [[5r21]], [[5r22]], [[5r23]], [[5r24]], [[5r25]], [[5r26]], [[5r27]], [[5r28]], [[5r29]], [[5r2a]], [[5r2b]], [[5r2c]], [[5r2d]], [[5r2e]], [[5r2f]], [[5r2g]], [[5r2h]], [[5r2i]], [[5r2j]], [[5r2k]], [[5r2l]], [[5r2m]], [[5r2n]], [[5r2o]], [[5r2p]], [[5r2q]], [[5r2r]], [[5r2s]], [[5r2t]], [[5r2u]], [[5r2v]], [[5r2w]], [[5r2x]], [[5r2y]], [[5r2z]], [[5r30]], [[5r31]], [[5r32]], [[5r33]], [[5r34]], [[5r35]], [[5r36]], [[5r37]], [[5r38]], [[5r39]], [[5r3a]], [[5r3b]], [[5r3c]], [[5r3d]], [[5r3e]], [[5r3f]], [[5r3g]], [[5r3h]], [[5r3i]], [[5r3j]], [[5r3k]], [[5r3l]], [[5r3m]], [[5r3n]], [[5r3o]], [[5r3p]], [[5r3q]], [[5r3r]], [[5r3s]], [[5r3t]], [[5r3u]], [[5r3v]], [[5r3w]], [[5r3x]], [[5r3y]], [[5r3z]], [[5r40]], [[5r41]], [[5rbo]], [[5rbp]], [[5rbq]], [[5rbr]], [[5rbs]], [[5rbt]], [[5rbu]], [[5rbv]], [[5rbw]], [[5rbx]], [[5rby]], [[5rbz]], [[5rcl]], [[5rcm]], [[5rc4]], [[5rc3]], [[5rc2]], [[5rc5]], [[5rc6]], [[5rc7]], [[5rc0]], [[5rc1]], [[5rca]], [[5rcb]], [[5rcc]], [[5rcd]], [[5rch]], [[5rcl]], [[5rcm]], [[5rcq]], [[5rcs]], [[5rce]], [[5rcf]], [[5rcg]], [[5rcx]], [[5rcy]], [[5rcz]], [[5rd2]], [[5rd4]], [[5rd7]], [[5rd9]], [[5rda]], [[5rde]], [[5rdg]], [[5rdh]], [[5rdi]], [[5rdm]], [[5rdo]], [[5rdp]], [[5rdq]], [[5rdr]], [[5rds]], [[5rdt]], [[5rdu]], [[5rdv]], [[5rdw]], [[5rdx]], [[5rdy]], [[5rdz]], [[5re0]], [[5re1]], [[5re2]], [[5sak]], [[5sam]], [[5san]], [[5sao]], [[5sap]], [[5saq]], [[5sas]], [[5sat]], [[6ron]], [[6rsv]], [[6scv]], [[6zz2]], [[7adg]] – CpETPep + inhibitor<br /> | ||
**[[2vs2]], [[1gkt]] - CpETPep + inhibitor - neutron | **[[2vs2]], [[1gkt]] - CpETPep + inhibitor - neutron<br /> | ||
**[[3urj]] - ETPep – ''Endothia parasitica''<br /> | |||
}} | }} | ||
=References= | =References= | ||