3p27: Difference between revisions

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-{{Seed}}
-[[Image:3p27.png|left|200px]]
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+==Crystal structure of S. cerevisiae Hbs1 protein (GDP-bound form), a translational GTPase involved in RNA quality control pathways and interacting with Dom34/Pelota==
-The line below this paragraph, containing "STRUCTURE_3p27", creates the "Structure Box" on the page.
+<StructureSection load='3p27' size='340' side='right'caption='[[3p27]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3p27]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P27 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-{{STRUCTURE_3p27|  PDB=3p27  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p27 OCA], [https://pdbe.org/3p27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p27 RCSB], [https://www.ebi.ac.uk/pdbsum/3p27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p27 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/HBS1_YEAST HBS1_YEAST] Involved in protein translation. Together with DOM34, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs.<ref>PMID:16554824</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Eukaryotic cells have several quality control pathways that rely on translation to detect and degrade defective RNAs. Dom34 and Hbs1 are two proteins that are related to translation termination factors and are involved in no-go decay (NGD) and nonfunctional 18S ribosomal RNA (rRNA) decay (18S NRD) pathways that eliminate RNAs that cause strong ribosomal stalls. Here we present the structure of Hbs1 with and without GDP and a low-resolution model of the Dom34-Hbs1 complex. This complex mimics complexes of the elongation factor and transfer RNA or of the translation termination factors eRF1 and eRF3, supporting the idea that it binds to the ribosomal A-site. We show that nucleotide binding by Hbs1 is essential for NGD and 18S NRD. Mutations in Hbs1 that disrupted the interaction between Dom34 and Hbs1 strongly impaired NGD but had almost no effect on 18S NRD. Hence, NGD and 18S NRD could be genetically uncoupled, suggesting that mRNA and rRNA in a stalled translation complex may not always be degraded simultaneously.
-===Crystal structure of a translational GTPase (GDP form)===
+Dissection of Dom34-Hbs1 reveals independent functions in two RNA quality control pathways.,van den Elzen AM, Henri J, Lazar N, Gas ME, Durand D, Lacroute F, Nicaise M, van Tilbeurgh H, Seraphin B, Graille M Nat Struct Mol Biol. 2010 Dec;17(12):1446-52. Epub 2010 Nov 21. PMID:21102444<ref>PMID:21102444</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_21102444}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3p27" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 21102444 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21102444}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-P27 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P27 OCA].
-==Reference==
-<ref group="xtra">PMID:21102444</ref><references group="xtra"/>
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Durand, D.]]
+[[Category: Durand D]]
-[[Category: Elzen, A van den.]]
+[[Category: Gas ME]]
-[[Category: Gas, M E.]]
+[[Category: Graille M]]
-[[Category: Graille, M.]]
+[[Category: Henri J]]
-[[Category: Henri, J.]]
+[[Category: Lacroute F]]
-[[Category: Lacroute, F.]]
+[[Category: Lazar N]]
-[[Category: Lazar, N.]]
+[[Category: Nicaise M]]
-[[Category: Nicaise, M.]]
+[[Category: Sraphin B]]
-[[Category: Sraphin, B.]]
+[[Category: Van Tilbeurgh H]]
-[[Category: Tilbeurgh, H van.]]
+[[Category: Van den Elzen A]]
-[[Category: YSG, Paris-Sud Yeast Structural Genomics.]]
-[[Category: Beta-barrel]]
-[[Category: Gdp/gtp binding domain]]
-[[Category: Paris-sud yeast structural genomic]]
-[[Category: Signaling protein]]
-[[Category: Structural genomic]]
-[[Category: Translational gtpase]]
-[[Category: Ysg]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 15 08:11:41 2010''