3otg: Difference between revisions

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-{{Seed}}
-[[Image:3otg.jpg|left|200px]]
-<!--
+==Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP bound form==
-The line below this paragraph, containing "STRUCTURE_3otg", creates the "Structure Box" on the page.
+<StructureSection load='3otg' size='340' side='right'caption='[[3otg]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3otg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTG FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.08&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
-{{STRUCTURE_3otg|  PDB=3otg  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3otg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otg OCA], [https://pdbe.org/3otg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3otg RCSB], [https://www.ebi.ac.uk/pdbsum/3otg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3otg ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8KNF2_MICEC Q8KNF2_MICEC]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Glycosyltransferases are useful synthetic catalysts for generating natural products with sugar moieties. Although several natural product glycosyltransferase structures have been reported, design principles of glycosyltransferase engineering for the generation of glycodiversified natural products has fallen short of its promise, partly due to a lack of understanding of the relationship between structure and function. Here, we report structures of all four calicheamicin glycosyltransferases (CalG1, CalG2, CalG3, and CalG4), whose catalytic functions are clearly regiospecific. Comparison of these four structures reveals a conserved sugar donor binding motif and the principles of acceptor binding region reshaping. Among them, CalG2 possesses a unique catalytic motif for glycosylation of hydroxylamine. Multiple glycosyltransferase structures in a single natural product biosynthetic pathway are a valuable resource for understanding regiospecific reactions and substrate selectivities and will help future glycosyltransferase engineering.
-===Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP bound form===
+Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.,Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN Jr Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17649-54. Epub 2011 Oct 10. PMID:21987796<ref>PMID:21987796</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-OTG is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTG OCA].
+<div class="pdbe-citations 3otg" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Micromonospora echinospora]]
-[[Category: Bingman, C A.]]
+[[Category: Bingman CA]]
-[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
+[[Category: Chang A]]
-[[Category: Chang, A.]]
+[[Category: Phillips Jr GN]]
-[[Category: Phillips, G N.]]
+[[Category: Singh S]]
-[[Category: Singh, S.]]
+[[Category: Thorson JS]]
-[[Category: Thorson, J S.]]
-[[Category: Calicheamicin]]
-[[Category: Center for eukaryotic structural genomic]]
-[[Category: Cesg]]
-[[Category: Glycosyltransferase]]
-[[Category: Gt-b fold]]
-[[Category: Protein structure initiative]]
-[[Category: Psi-2]]
-[[Category: Structural genomic]]
-[[Category: Tdp]]
-[[Category: Transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 15 08:43:52 2010''