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<StructureSection load='3gpt' size='400' side='right' scene='' caption='Yeast proteasome 20S core complex with salinosporamide derivative, [[3gpt]]'>
<StructureSection load='' size='350' side='right' scene='41/417543/Cv/1' caption='Yeast proteasome 20S core complex with salinosporamide derivative, [[3gpt]]'>
 
== Function ==
== Function ==
[[Proteasome]] (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides<ref>PMID:22350895</ref>.  The '''26S PRTS''' is composed of a central '''20S core particle''' which contains 4 stacked rings each with several members and two 19S caps.  The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which bind polyubiquitin. <br />
[[Proteasome]] (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides<ref>PMID:22350895</ref>.  The '''26S PRTS''' is composed of a central '''20S core particle''' which contains 4 stacked rings each with several members and two 19S caps.  The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which bind polyubiquitin. <br />
*'''13S PRTS''' is a PRTS core intermediate containing α ring, partial β ring and 3 chaperones<ref>PMID:33846632</ref>.
*'''15S PRTS''' is half of an 20S PRTS and upon dimerisation forms the mature 20S PRTS<ref>PMID:32442437</ref>.
For more details see [[3unb]].
For more details see [[3unb]].


== Structural highlights ==
== Structural highlights ==
The core particle two outer rings contain 7 α subunits (Y7, Y13, PRE6, PRE5, PUP2, C1, C7-α) which form the PRTS gate.  The two inner rings contain 7 β subunits (PRE2, PRE4, PRE3, PUP1, PUP3, C5, C11) with protease activity<ref>PMID:8811196</ref>.
The core particle two outer rings contain 7 α subunits (Y7, Y13, PRE6, PRE5, PUP2, C1, C7-α) which form the PRTS gate.  The two inner rings contain 7 β subunits (PRE2, PRE4, PRE3, PUP1, PUP3, C5, C11) with protease activity<ref>PMID:8811196</ref>.
*<scene name='41/417543/Cv/2'>α subunits and β subunits</scene>.
*<scene name='41/417543/Cv/6'>Salinosporamide derivative binding site</scene> of chain H.
*<scene name='41/417543/Cv/7'>Covalent bond between Salinosporamide derivative and Thr1</scene>.
 
== 3D Structures of Proteasome ==
[[Proteasome 3D structures]]
== See Also ==
[[PROTAC]] (Proteolysis-Targeting Chimera), an alternative to conventional enzyme inhibitors, by enabling proteasome induced degradation of the target protein.
</StructureSection>
</StructureSection>
== 3D Structures of Proteasome ==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
{{#tree:id=OrganizedByTopic|openlevels=0|
* Proteasome 20S particle
**[[3mfe]], [[3mi0]], [[2fhg]] – Mt20S core – ''Mycobacterium tuberculosis''<br />
**[[3gpt]], [[3gpw]], [[1g0u]] – y20S core - yeast<br />
**[[1ryp]], [[3wxr]], [[4qux]], [[4quy]], [[4qv0]], [[4qv1]], [[4qv3]], [[4qv4]], [[4qv5]], [[4qv6]], [[4qv7]], [[4qv8]], [[4qv9]] - y20S core (mutant)<br />
**[[4r3o]] – h20S – human<br />
**[[3mka]], [[3hfa]] – Mt20S core (mutant)<br />
**[[3c91]], [[3c92]], [[1pma]], [[3j9i]] – Ta20S core - ''Themoplasma acidophilum''<br />
**[[3h4p]] – 20S core – ''Methanocaldococcus janaschii''<br />
**[[2h6j]], [[1q5r]] – Re20S core (mutant) – ''Rhodococcus erythropolis''<br />
**[[1q5q]] - Re20S core<br />
**[[1j2q]] – Af20S core - ''Archaeoglobus fulgidus''<br />
**[[1iru]] – 20S core - bovine<br />
**[[3une]] – m20S core – mouse<br />
**[[3unh]] – m20S core (immuno)
*Proteasome 20S+activator protein
**[[1vsy]], [[3l5q]] – y20S core+ Blm10 <br />
**[[1z7q]], [[1fnt]] – y20S core+PA26<br />
**[[3ipm]], [[3jrm]], [[3jse]], [[3jtl]], [[1ya7]], [[1yar]], [[1yau]] - Taa-PRTS chains A-G+ Tab-PRTS chains H-N+PA26 chains O-U <br />
*Proteasome 20S+inhibitor
**[[3hye]], [[3gpj]], [[3dy3]], [[3dy4]], [[3e47]], [[3d29]], [[3bdm]], [[2zcy]], [[2gpl]], [[2fak]], [[2f16]], [[1jd2]], [[1g65]], [[3mg0]], [[3mg4]], [[3mg6]], [[3mg7]], [[3mg8]], [[3oeu]], [[3oev]], [[3okj]], [[3shj]], [[3un4]], [[3un8]], [[3sdi]], [[3sdk]], [[4fzc]], [[4fzg]], [[4gk7]], [[4eu2]], [[4inr]], [[4int]], [[4inu]], [[4jsq]], [[4jsu]], [[4jt0]], [[4qwx]], [[4qzz]], [[4r17]], [[4r18]], [[4rur]], [[5ahj]] – y20S core+inhibitor<br />
**[[4qxj]], [[4qz0]], [[4qz1]], [[4qz2]], [[4qz3]], [[4qz4]], [[4qz5]], [[4qz6]], [[4qz7]], [[4qzw]], [[4qzx]], [[4r00]], [[4r02]]  – y20S (mutant) + inhibitor<br />
**[[3tdd]], [[4j70]] - y20S core + belactosin<br />
**[[4hnp]], [[4hrc]], [[4hrd]], [[4lqi]], [[4ltc]], [[4q1s]] - y20S core + antibiotics<br />
**[[4nnn]], [[4nnw]], [[4no1]], [[4no6]], [[4no8]], [[4no9]], [[4qby]], [[4qlq]], [[4qls]], [[4qlt]], [[4qlu]], [[4qlv]] - y20S core+ peptide inhibitor<br />
**[[3h6f]], [[3h6i]], [[3hf9]], [[2fhh]], [[3krd]] – Mt20S core+inhibitor<br />
**[[3nzj]], [[3nzw]], [[3nzx]] - y20S + TMC-95A mimic ligand<br />
**[[2zcy]] – y20S+syringolin<br />
**[[4qvl]], [[4qw4]] – y20S + lymphoma drug<br />
**[[4qvm]], [[4qvn]], [[4qvp]], [[4qvq]], [[4qvv]], [[4qvw]], [[4qvy]], [[4qw0]], [[4qw1]], [[4qw3]], [[4qw5]], [[4qw6]], [[4qw7]], [[4qwf]], [[4qwg]], [[4qwi]], [[4qwj]], [[4qwk]], [[4qwl]], [[4qwr]], [[4qws]], [[4qwu]] – y20S (mutant) + lymphoma drug<br />
**[[4r67]] – h20S + cancer drug<br />
**[[3unb]], [[3unf]] – m20S + inhibitor
* Proteasome partial 20S particle
**[[2ku1]], [[2ku2]] – Taa-PRTS chains A-G <br />
**[[3fp9]] – MtATPase chains A-L<br />
**[[1j2p]] – Af α subunit<br />
**[[2jay]] – Mt β subunit<br />
**[[2kqz]], [[2kr0]] – hPRTS ubiquitin receptor domain
*Proteasome partial 26S particle
**[[1p9c]] – hPRTS subunit 4 C-terminal<br />
**[[1p9d]] - hPRTS subunit 4 C-terminal + HHR23A ubiquitin-like domain<br />
**[[1uel]] - hPRTS subunit 4 C-terminal + HHR23A ubiquitin-like domain - NMR<br />
**[[1yx4]] - hPRTS subunit 4 – NMR<br />
**[[1yx5]], [[1yx6]], [[2kde]], [[2kdf]] - hPRTS subunit 4+ubiquitin – NMR<br />
**[[2o95]], [[2o96]] - hPRTS subunit 7<br />
**[[3kw6]] - hPRTS subunit 8<br />
**[[2krk]] - hPRTS subunit 8 – NMR<br />
**[[1qym]], [[1uoh]] - hPRTS subunit 10<br />
**[[1tr4]] - hPRTS subunit 10 – NMR<br />
**[[4nik]] - hPRTS subunit 10 + single-chain Fv<br />
**[[3t5x]] - hPRTS subunit Dss1 + PCI domain-containing protein 2<br />
**[[2dvw]], [[2dwz]] - mPRTS subunit 10 + hPRTS subunit 6B – mouse<br />
**[[3aji]] - mPRTS subunit 10 +  subunit 4<br />
**[[2x5n]] - PRTS subunit RPN10 VWA domain – fission yeast<br />
**[[4ady]] - yPRTS subunit RPN2<br />
**[[3vlf]] - yPRTS subunit 7 + DNA mismatch repair protein<br />
**[[2mr3]] - yPRTS subunit RPN9 – NMR<br />
**[[2mqw]] - yPRTS subunit RPN9 N terminal – NMR<br />
**[[2mri]] - yPRTS subunit RPN9 C terminal – NMR<br />
**[[4b0z]] - yPRTS subunit RPN12<br />
**[[2z4d]] - yPRTS subunit RPN13<br />
**[[3vl1]] - yPRTS subunit RPN14 (mutant)<br />
**[[4o8x]], [[4o8y]] - yPRTS subunit RPN8 + RPN11<br />
**[[4ocl]], [[4ocm]], [[4ocn]] - yPRTS subunit RPN8 + RPN11 + nanobody<br />
**[[3t5v]] - yPRTS subunit SEM1 + nuclear mRNA export proteins SAC3 and THP1<br />
**[[2z59]] - mPRTS subunit RPN13+ubiquitin<br />
**[[3txm]], [[3txn]] – PRTS subunit P42B – ''Drosophila melanogaster''
*Proteasome 26S particle


**[[4b4t]], [[3j47]], [[4cr2]], [[4cr3]], [[4cr4]] – yPRTS – Cryo EM<br />
**[[4g4s]] – yPRTS + proteasome assembly chaperon 1 & 2<br />
}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Michal Harel, Joel L. Sussman, Jaime Prilusky
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